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Populus alba × P . grandidentata 조직배양묘의 (組織培養苗) 토양에서의 활착과 생장
전영우,Richard B . Hall ( Young Woo Chun,Richard B . Hall ) 한국산림과학회 1984 한국산림과학회지 Vol.66 No.1
This study was undertaken to find out the effects of three kinds of potting media and two sources of explants on the survival and early growth of new plantlets of Poputus alba × P. grandidentata in the greenhouse. The results obtained can be summarized as follows; 1) Among three potting media, Terralite was best for early growth and survival of plantlets. 2) Like humidifier, an intermittent misting system can be effective in keeping relative humidity high for the plantlets. 3) Survival rates over 80% could be obtained if humidity was kept high during the hardening period. 4) During hardening period, the plantlets showed the juvenile characteristics such as smaller leaves, thinner stems, and shorter internodes. 5) There were no differences on morphological characteristics between the plantlets originating from axillary buds and the plantlets originating from multiple shoots while they were growing at the greenhouse. 6) The plantlets originating from bud culture grew normally comparing to regular cuttings.
姜鎬德,朴敎秀,Richard B. Hall 동국대학교 생명자원과학대학 연습림 1995 연습림논문집 Vol.- No.4
교잡종 포플러 (Canada blanc, Eugenei, Ⅰ-45/51, Wisconsin #5)의 기내증식에 미치는 요인들이 조사되어졌다. 식물성장 조절물질로서 싸이토키닌류와 오옥신류와의 조합은 뿌리, 줄기, 잎의 시료로부터 기내증식의 효율 증대에 영향을 미치었다. 일반적으로 benzylaminopurine (BA)과 오옥신과의 조합은 Canada blanc 클론의 잎 시료에 매우 효과적 이었고, zeation과 오옥신류와의 조합은 Wisconsin #5 클론의 줄기 시료에 매우 중요하게 작용했다. 각 시료에서 최대의 증식되어진 줄기의 수는 BA 2.0 ㎎/l를 처리 했을때 Canada blanc의 뿌리 시료로 부터 17.60±8.96개가 생산되어졌다. 반면에, Wisconsin #5의 줄기와 Canada blanc의 잎은 zeation/ 2.0㎎/l와 IAA 0.2㎎/l의 조합과/ BA0.5㎎/l와 2.4-D 0.05㎎/l의 조합에서 각각 18.20±3.03개와 17.80±1.92개의 줄기들을 증식시켰다. 특히, 싸이토키닌의 들어있는 배지에 2.4-D의 첨가는 줄기증식을 촉진시켰다.
Polyploidy induction in the crandon clone of Populus alba × P. grandidentata by colchicine treatment
전영우(Young Woo Chun),Richard B. Hall 한국육종학회 1992 한국육종학회지 Vol.24 No.2
Crandon clone of Populus alba×P. grandidentata was treated with various colchicine concentrations and treatment durations to determine the best method for inducing polyploidy through in vitro treatment of axillary buds. Colchiploid shoots were only produced from in vivo, but not from in vitro colchicine treatments. Most colchiploid shoots originated from buds that were treated 24 h. duration with 1% colchicine solution and 48 h. duration with 2% colchicine solution. Twelve colchiploid shoots produced from in vivo colchicine treatment continued to exhibit the chimera leaf shape after cuttings and have mixed chromosome numbers, 2n (38) and 4n (76). The adventitious shoots produced from chimeric leaf discs through in vitro culture appeared either normal or colchiploid leaf shape with thicker stem diameter. The colchiploid adventitious shoots had the mixed 2n-4n chromosome numbers.
손성호(Sung Ho Son),Richard B. Hall 한국육종학회 1993 한국육종학회지 Vol.25 No.3
Successful acclimatization from in vitro shoot cultures of hybrid aspen were obtained using Polyterra Peat Plug System(PPPS) and Artificial Soil Mix(ASM) under two types of humidity control system such as Intermittent Misting System(IMS) and Gas Exchangeable Humidity Tent(GEHT). Best results(more than 98% survival) were observed by transplanting well-expanded(8 ㎝ in height) in vitro shoot cultures onto PPPS and maintained in the greenhouse where IMS and anti-radiation screen were installed. When acclimated plants were re-poted in round standard plastic pots containing ASM, each shoots started rapid growth with no more death. Reliable two step methods for hardening of large-scale micropropagules using this hybrid aspen was successfully demonstrated.
Kang, Hoduck,Cheong, Yong-Moon,Hall, Richard B. 公州大學校 基礎科學硏究所 1995 自然科學硏究 Vol.4 No.-
포플러의 기내 줄기 증식과 줄기, 뿌리 성장에 영향을 주는 유기, 무기 질소원의 효과가 조사 되어졌다. 질소원의 형태와 농도는 줄기의 증식과 성장에 매우 중요한 요인들로 작용했다. 대량의 줄기들이 BAP 0.2 mg/L를 함유하고 무기물의 다양한 농도를 포함하고 있는 MS 배지에서 axillary bud와 shoot tip으로 부터 유기 되어졌다. Axillary bud는 기내배양에서 평균 12.67개의 줄기가 유기 되어졌고, shoot tip은 이보다 적은 평균 7.50개의 줄기를 생산 시켰다. MS 배지에서 암모니아태 질소의 반감은 줄기 증식과 줄기 성장을 촉진 시켰으며, 암모니움태와 질산태 조성의 반감은 보다 많은 줄기의 생산에 효과적 이었다. MS 배지에 아미노산의 첨가는 또한 기내배양에서 줄기의 수를 증가 시켰으며, 아미노산중 arginine은 axillary bud와 glycine은 shoot tip에 중요한 인자로 작용했다. MS 배지에서 arginine과 glycine의 최적 농도는 20 mg/L였고 이들은 각각 평균 13.33과 5.17개의 줄기를 생산 시켰다. The effects of organic and inorganic nitrogen on shoot multiplication and shoot and root development of Populus alba X P. grandidentata were investigated. The types of nitrogen sources and their concentrations significantly affected shoot proliferation and shoot growth. Multiple shoots were obtained from axillary buds and shoot tips as explants on MS medium containing various concentrations of inorganic nitrogen (NH4NO3 and KNO3) and 0.2 mg/l BA. Axillary buds (12.67) produced more shoots than shoot tip explants (7.50) on the medium. A reduction of NH4NO3 (ammonium nitrate) from full-strength to half-strength promoted shoot multiplication and shoot growth. A half strength combination of NH4NO3 and KNO3 produced more shoots than the full strength combination of these inorganic nitrogen sources. The best amino acid supplement was arginine with respect to the production of multiple shoots in axillary bud explants and was glycine in shoot tip explants. The greatest numbers of shoots were 13.33 on the medium containing arginine at 20 mg/l and 5.17 on the medium containing glycine at 20 mg/l.
전영우 ( Young Woo Chun,Ned B . Klopfenstein,Harold S . McNabb Jr .,Richard B . Hall ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.4
The development of tissue culture systems for Populaes species, and the utilization of tissue culture biotechnology will be reviewed and discussed. Special emphasis will be placed on prospects for genetic transformation by Agrobacterium-mediated gene transfer methods.