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Polyploidy induction in the crandon clone of Populus alba × P. grandidentata by colchicine treatment
전영우(Young Woo Chun),Richard B. Hall 한국육종학회 1992 한국육종학회지 Vol.24 No.2
Crandon clone of Populus alba×P. grandidentata was treated with various colchicine concentrations and treatment durations to determine the best method for inducing polyploidy through in vitro treatment of axillary buds. Colchiploid shoots were only produced from in vivo, but not from in vitro colchicine treatments. Most colchiploid shoots originated from buds that were treated 24 h. duration with 1% colchicine solution and 48 h. duration with 2% colchicine solution. Twelve colchiploid shoots produced from in vivo colchicine treatment continued to exhibit the chimera leaf shape after cuttings and have mixed chromosome numbers, 2n (38) and 4n (76). The adventitious shoots produced from chimeric leaf discs through in vitro culture appeared either normal or colchiploid leaf shape with thicker stem diameter. The colchiploid adventitious shoots had the mixed 2n-4n chromosome numbers.
Agrobacterium Binary Vector 에 의한 포플러 형질전환을 위한 기초연구
전영우 ( Young Woo Chun,Ned B . Klopfenstein,Harold S . McNabb,Jr .,Richard B . Hall ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.2
Three clones of Populus alba × P. grandidemtata have been tested for susceptibility to Agrobacterium tumefaciens strains A281 and A348. We determined the optimum concentration of kanamycin sulfate for effective selection of leaf disc-derived, transgenic tissues transformed using Agrobacterium binary vector pGA472 containing a neomycin phosphotransferase gene (NPT-II) which confers kanamycin resistance. Of the wild type Ti plasmids contained by the two Agrobacterium strains, pTiBo542 of strain A281 appears Co be best suited to serve as a helper plasmid for binary vector systems. A relatively low concentration (10㎎/ℓ) of kanamycin sulfate inhibited adventitious shoot initiation from leaf discs on regeneration medium. Transformed kanamycin-resistant calli were obtained by culturing Agrobacterium inoculated leaf discs on selective regeneration medium. The transformed kanamycin-resistant calli continued to grow on regeneration media supplemented with kanamycin sulfate to levels of 50 and 204㎎/ℓ. The growth of non-cultivated control calli was severely inhibited on regeneration medium containing 50㎎/ℓ kanamycin sulfate.
Populus alba × P . grandidentata 조직배양묘의 (組織培養苗) 토양에서의 활착과 생장
전영우,Richard B . Hall ( Young Woo Chun,Richard B . Hall ) 한국산림과학회 1984 한국산림과학회지 Vol.66 No.1
This study was undertaken to find out the effects of three kinds of potting media and two sources of explants on the survival and early growth of new plantlets of Poputus alba × P. grandidentata in the greenhouse. The results obtained can be summarized as follows; 1) Among three potting media, Terralite was best for early growth and survival of plantlets. 2) Like humidifier, an intermittent misting system can be effective in keeping relative humidity high for the plantlets. 3) Survival rates over 80% could be obtained if humidity was kept high during the hardening period. 4) During hardening period, the plantlets showed the juvenile characteristics such as smaller leaves, thinner stems, and shorter internodes. 5) There were no differences on morphological characteristics between the plantlets originating from axillary buds and the plantlets originating from multiple shoots while they were growing at the greenhouse. 6) The plantlets originating from bud culture grew normally comparing to regular cuttings.
전영우 ( Young Woo Chun,Ned B . Klopfenstein,Harold S . McNabb Jr .,Richard B . Hall ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.4
The development of tissue culture systems for Populaes species, and the utilization of tissue culture biotechnology will be reviewed and discussed. Special emphasis will be placed on prospects for genetic transformation by Agrobacterium-mediated gene transfer methods.