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Rao Ravella Sreenivas,Jyothi Cherukuri Pavanna,Prakasham Reddy Shetty,Rao Chaganti Subba,Sarma Ponnupalli Nageshwara,Rao Linga Venkateswar The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.1
Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamvdospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.
Linga Venkateswar Rao,Cherukuri Pavana Jyothi,Reddy Shetty Prakasham,Chaganti Subba Rao,Ponnupalli Nageshwara Sarma,Ravella Sreenivas Rao 한국미생물학회 2006 The journal of microbiology Vol.44 No.1
Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamydospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.
Elucidating acetogenic H<sub>2</sub> consumption in dark fermentation using flux balance analysis
Lalman, Jerald A.,Chaganti, Subba Rao,Moon, Chungman,Kim, Dong-Hoon Elsevier 2013 Bioresource technology Vol.146 No.-
<P><B>Abstract</B></P> <P>In this study, a flux balance analysis (FBA) was adopted to estimate the activity of acetogenic H<SUB>2</SUB>-consuming reaction. Experimental data at different substrate concentrations of 10, 20, and 30g COD/L showing the lowest, medium, and highest H<SUB>2</SUB> yields, respectively, were used in the FBA to calculate the fluxes. It was interesting to note that the hydrogenase activity based on R12 (2Fd<SUP>+</SUP> +2H<SUP>+</SUP> →2Fd<SUP>2+</SUP> +H<SUB>2</SUB>, ferredoxin (Fd)) flux was most active at 10g COD/L. The flux of R17 (4H<SUB>2</SUB> +2CO<SUB>2</SUB> →CH<SUB>3</SUB>COOH), a mechanism for reutilizing produced H<SUB>2</SUB>, increased in steps of 0.030, 0.119, and 0.467 as the substrate concentration decreased. Contradictory to our general understanding, acetate production found to have a negligible or even negative effect on the final H<SUB>2</SUB> yield in dark fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Revealing the acetogenic H<SUB>2</SUB>-consuming reaction using FBA in dark fermentation. </LI> <LI> Reutilization of produced H<SUB>2</SUB> by acetogenic H<SUB>2</SUB>-consuming reaction was crucial. </LI> <LI> Acetate production has a negligible or even negative effect on the final H<SUB>2</SUB> yield. </LI> </UL> </P>