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RISS 인기검색어
- 검색키워드
- 저자 : Piao Xuanjing (검색결과 2 건)
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Yao, Xuerui,Jiang, Hao,NanXu, Yong,Piao, Xuanjing,Gao, Qingshan,Kim, Nam-Hyung Butterworths, etc 2019 Theriogenology Vol. No.
<P><B>Abstract</B></P> <P>Kaempferol (3,4′,5,7-tetrahydroxyflavone, KAE) is one of the most commonly occurring dietary flavonols. The biological and pharmacological effects of kaempferol depend upon whether it acts as an antioxidant, anti-inflammatory, or anticancer agent. The present study explored the influence of KAE supplementation on <I>in vitro</I> damage to porcine oocytes and its underlying mechanisms. Different concentrations of KAE (0.05, 0.1, 0.5, 1 μM) were added to porcine zygote medium 5 during <I>in vitro</I> culture. The results showed that supplementation with 0.1 μM KAE significantly increased the blastocyst formation rate. Blastocyst formation and quality were significantly increased in the 200 μM H<SUB>2</SUB>O<SUB>2</SUB> treatment group following addition of 0.1 μM KAE. KAE prevented the H<SUB>2</SUB>O<SUB>2</SUB>-induced compromise of mitochondrial membrane potential and reactive oxygen species generation. Furthermore, the extent of autophagy and DNA damage in the blastocysts was attenuated by supplementation with KAE in the H<SUB>2</SUB>O<SUB>2</SUB> induced oxidative injury group compared to that observed in controls. These results suggest that KAE has beneficial effects on the development of porcine parthenotes by attenuating oxidative stress and increasing mitochondrial function.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Kaempferol has beneficial effects on the development of porcine early stage embryos. </LI> <LI> Kaempferol prevented the H<SUB>2</SUB>O<SUB>2</SUB>-induced production of ROS. </LI> <LI> Kaempferol prevented mitochondrial dysfunction induced by H<SUB>2</SUB>O<SUB>2</SUB> in porcine embryos. </LI> <LI> Kaempferol prevented H<SUB>2</SUB>O<SUB>2</SUB>-induced autophagy and DNA damage. </LI> </UL> </P>
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Kwon Jeongwoo,Seong Min-Jung,Piao Xuanjing,Jo Yu-Jin,김남형 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10
Objective: This study was conducted to investigate the roles of LIM kinases (LIMK1 and LIMK2) during porcine early embryo development. We checked the mRNA expression patterns and localization of LIMK1/2 to evaluate their characterization. We further explored the function of LIMK1/2 in developmental competence and their relationship between actin assembly and cell junction integrity, specifically during the first cleavage and compaction. Methods: Pig ovaries were transferred from a local slaughterhouse within 1 h and cumulus oocyte complexes (COCs) were collected. COCs were matured in in vitro maturation medium in a CO2 incubator. Metaphase II oocytes were activated using an Electro Cell Manipulator 2001 and microinjected to insert LIMK1/2 dsRNA into the cytoplasm. To confirm the roles of LIMK1/2 during compaction and subsequent blastocyst formation, we employed a LIMK inhibitor (LIMKi3). Results: LIMK1/2 was localized in cytoplasm in embryos and co-localized with actin in cell-to-cell boundaries after the morula stage. LIMK1/2 knockdown using LIMK1/2 dsRNA significantly decreased the cleavage rate, compared to the control group. Protein levels of E-cadherin and β-catenin, present in adherens junctions, were reduced at the cell-to-cell boundaries in the LIMK1/2 knockdown embryos. Embryos treated with LIMKi3 at the morula stage failed to undergo compaction and could not develop into blastocysts. Actin intensity at the cortical region was considerably reduced in LIMKi3-treated embryos. LIMKi3-induced decrease in cortical actin levels was attributed to the disruption of adherens junction and tight junction assembly. Phosphorylation of cofilin was also reduced in LIMKi3-treated embryos. Conclusion: The above results suggest that LIMK1/2 is crucial for cleavage and compaction through regulation of actin organization and cell junction assembly.
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