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인위적인 원형질막과 세포내 저밀도막의 접합에 미치는 제4형 당운반체 단백질의 전이 및 조절인자에 대한 연구
조용욱,김창세,Paul F. Pilch 순천향대학교 1994 논문집 Vol.17 No.2
We tried to induce the fusion of plasma membrane and intracellular low density microsomal membrane, and evaluated about the regulatory factors(insulin, cytosol, ATP, GTP, incubation time and temperature) which may affect the fusion and movement of the glucose transporter 4(GLUT 4). We obtained the results as follows; 1) Membrane-associated fusion might be fairely well established, and we could confiremd that fact by western analysis. 2) Insulin stimulated the fusion and movement of the GLUT 4. 3) Cytosol showed the tendency to increase the fusion and movement of the GLUT 4, but its effect was not apparent. 4) Other regulatory factor such as ATP, GTP, incubation time and temperature did not affect to the fusion and movement of the GLUT 4. 5) There might be somewhat non-specific reaction when separate the plasma mem-brane and intracellular low density microsomal membrane after fusion. In conclusion, insulin apparently stimulated the fusion and movement of the GLUT 4, but ATP, GTP, incubtion time and temperature did not. Further study to reseparate more specifically the PM and LDM after fusion must be required to clarify the exact effects of various regulatory factors. Acknowledgments : The author thanks Prof. Paul F. Pilch for advice about manu-script and supply the monoclonal antobody of GLUT 4(1F8).