http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Muthuramalingam, Karthika,Kim, Seo-Young,Kim, Youngmee,Kim, Hyun-Soo,Jeon, You-Jin,Cho, Moonjae ELSEVIER SCIENCE B.V.; AMSTERDAM 2019 JOURNAL OF FUNCTIONAL FOODS Vol.52 No.-
<P><B>Abstract</B></P> <P>Big belly seahorse (<I>Hippocampus abdominalis</I>) is a well-known marine organism recognized for its pharmacological value. In this study, peptides derived from natively (Jeju-do, Republic of Korea) farmed seahorses were investigated for their potency on myoblast differentiation and endurance capacitance. Cell viability studies on C<SUB>2</SUB>C<SUB>12</SUB> mouse myoblast cells showed that more than 80% of the cells treated with peptides were metabolically active. Morphological observation illustrated the loss of characteristic radial branching towards elongated and well-developed muscle fibers in the peptide-treated C<SUB>2</SUB>C<SUB>12</SUB> cells. Immunoblot study on the <I>in vitro</I> translational expression level of key myogenic regulatory proteins (MyoD, MyoG, MyHC) and an <I>in vivo</I> endurance study (using zebrafish as a working model) demonstrated the significance of peptides on the myogenesis process and endurance swimming performance against water flow and gradient, respectively. Collectively, our findings suggest that seahorse-derived peptides can be used as a therapeutic nutrient supplement for improved endurance.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Hippocampus abdominalis</I> derived peptides enhances C<SUB>2</SUB>C<SUB>12</SUB> myoblast differentiation. </LI> <LI> These seahorse derived peptides modulated myogenic regulatory proteins. </LI> <LI> Translational analysis revealed concerted modulation at the P38MAPK/AKT axis. </LI> <LI> In zebrafish working model, these peptides improve endurance swimming performance. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Synthesis and optimization of immunomodulating hydrogel for biomedical application
( Karthika Muthuramalingam ),( Sanggyu Park ),( Moonjae Cho ) 한국응용생명화학회(구 한국농화학회) 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.4
Treatment towards wound healing, a complex and dynamic process, has been given a great deal of efforts in the last few decades. Focus has been imposed on developing wound dressings that meet the requirements for proper wound healing. In this study, hydrogel made from blends of poly (vinyl alcohol) and β-1,6-branched-β-1,3-glucan (beta-glucan) were synthesized by modified solvent casting method for wound dressing application. Optimization of hydrogel composition and analysis of wound dressing parameters such as stability and fluid uptake capacity (in the presence of water, saline and different pH solutions) has been studied. The result indicated that the PVA/beta-glucan hydrogel hold its structural integrity even at alkaline pH (pH~9) and upholds fluids four times of its original weight. Thus, the developed hydrogel is expected to be a promising candidate as wound dressing.
Effect of mushroom (Schizophyllum spp.) derived β-glucan on low-fiber diet induced gut dysbiosis
( Karthika Muthuramalingam ),( Vineet Singh ),( Changmin Choi ),( Seung In Choi ),( Sanggyu Park ),( Young Mee Kim ),( Tatsuya Unno ),( Moonjae Cho ) 한국응용생명화학회(구 한국농화학회) 2019 Journal of Applied Biological Chemistry (J. Appl. Vol.62 No.2
Dietary pattern has paramount importance in shaping the gut microbiota and its associated host health. Herein this study, long term (12 weeks) impact of mushroom derived dietary fiber, β-glucan, is investigated for its effect on low fiber diet consumption. Inclusion of dietary fiber into the low fiber diet (LFD) increased the abundance of genera Lactobacillus and Anaerostipes, the microbes responsible for butyrate (major ‘fuel source’ of colonocytes) production. Mice fed LFD with β-glucan showed significant increase in the length of small intestine compared to that of the LFD group without β-glucan. Further, dietary fiber consumption enhanced goblet cell density along with mucosal layer thickness. These results indicate promising effects of β-glucan towards maintenance of healthy gut and gut microbiota.
Karthika Muthuramalingam,조문제,김영미 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.1
Epithelial to mesenchymal transition (EMT) is a complex biological event, wherein polarized epithelial cells lose their integrity resulting in a mesenchymal phenotype with enhanced motility, a phenomenon known as metastasis. However, the underlying mechanisms of EMT are still poorly understood in cervical carcinomas. In this study, we investigated the molecular signalling events responsible for the effect of TGF-β, a potent inducer of EMT, on HeLa cervical cancer cells. We observed that TGF-β treatment (5 ng/mL) upregulates the expression of EMT-associated transcription factors such as Snail and Slug and downregulates the expression of epithelial markers such as ZO-1 and E-cadherin. Furthermore, treatment with TGF-β activates both Smad-dependent and Smad-independent signaling pathways, which subsides upon addition of Diphenyleneiodonium (DPI), a potent ROS inhibitor that inhibits NAPDH oxidase (NOX). TGF-β treatment enhanced cellular migration and invasion ability was diminished in the presence of ROS inhibitors. In addition, we also observed that ROS-mediated, TGF-β-induced EMT progression was inhibited using therapeutic candidates that target the key signal transduction mediators, including PI3K/AKT, ERK, and P38/MAPK. Accordingly, we demonstrated the involvement of redox biology (NOX2 and NOX4 mediate migration and invasion) in TGF-β-mediated EMT advancement and explored suitable therapeutic interventions.
( Karthika Muthuramalingam ),( Jun Ho Kim ),( You Jin Jeon ),( Sum Rho ),( Young Mee Kim ),( Moonjae Cho ) 한국응용생명화학회(구 한국농화학회) 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.4
Hippocampus abdominalis, the big belly sea horse, is widely known for its medicinal value in Chinese folk medicine. In this study, extract obtained by proteolytic degradation of this species was investigated for its effects on skeletal muscle development, both in vitro and in vivo. Muscle cell lines (C<sub>2</sub>C<sub>12</sub> and L<sub>6</sub>) treated with the bioactive peptide did not have any detrimental effects on the cell viability, which was above 80%. Optical microscopy analysis on the morphology of the sea horse extract (SHE)-treated cells showed enhanced differentiating ability with myotube formation. Moreover, cells incubated with the hydrolysate displayed decreased proliferation rate, as recorded by the electric cell substrate impedance sensing system, thereby supporting enhanced differentiation. For a period of 12 weeks, mice models were fed with SHE and simultaneously subjected to treadmill exercise, which increased the expression of Myogenin, a key myogenic regulatory factor. In addition, there was an increase in the expression of AMPK- and Cytochrome C, both of which are important in mitochondrial biogenesis. Thus, the SHE from Hippocampus abdominalis can be a promising candidate as protein supplement aiding muscle development.
Enhancement of filtration efficacy for particulate matters using β-glucan coated commercial masks
( Karthika Muthuramalingam ),( Young Mee Kim ),( Moonjae Cho ) 한국응용생명화학회(구 한국농화학회) 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.1
Ambient air pollution, in particular, particulate matter (PM) pollution imposes serious health concerns such as hospitalization and premature deaths, worldwide. While commercial breathing masks are in use for protection against this hazardous issue, yet their efficiency in filtering PM was not up to the par, besides several other discomforts such as poor breathability due to reduced air flow, sweat production etc. In this study, commercial face mask coated with β-glucan, a high molecular weight polymer is tested for its efficacy in filtering PM. Quantification of PM before and after filtration and microscopic observation (using scanning electron microscopy (SEM)) of the fabric used in filtering the dust pollutants (generated from wood chips and cigarette) showed that β-glucan coated fabric were significantly efficient in capturing PM (size of 10 and 2.5 μm in diameter) than that of the untreated control fabric, wherein the former had filtration efficacy with fold increase of 11.6 and 2.6 towards capturing PM2.5 and PM10 respectively than the latter. Thus, β- glucan coated fabric was found to be effective in filtering PM.
Emerging Research Advancements to Overcome the Peach Spring Frost
Pandiyan Muthuramalingam,Rajendran Jeyasri,Yeonju Park,Seongho Lee,Jae Hoon Jeong,Yunji Shin,Jinwook Kim,Sangmin Jung,Hyunsuk Shin 한국식물병리학회 2023 식물병연구 Vol.29 No.3
The phenomena of global warming has led to an increase in the average air temperature in temperate climates. Springtime frost damage is becoming more common, and after a period of dormancy, damage to buds, blooms, and developing fruits is greater significant than damage from low winter temperatures. Peaches are a crucial crop among moderate fruits. Spring frost damage in peaches can have a negative effect on crop growth, yield, and quality. It is noteworthy that these plants have evolved defenses against spring frost damage while being exposed to a variety of low temperatures in the early spring. In this current review, recent research advancements on spring frost damage avoidance in peaches were deliberated. Additionally, adaptive mechanisms of peach, such as deacclimation and reacclimation, were emphasized. Moreover, the emerging advancements using various omics approaches revealed the peach physiology and molecular mechanisms comprehensively. Furthermore, the use of chemical products and understanding the spring frost mechanisms through the use of environmental chamber temperature stimulation and infrared thermography studies were also discussed. This review is essential groundwork and paves the way to derive and design future research for agronomists and horticulturalists to overcome the challenges of spring frost damage avoidance and crop management in these circumstances.
Singh Vineet,Muthuramalingam Karthika,Kim Young Mee,Park Sanggyu,Kim Sung Hong,Lee Jongdae,Hyun Changlim,Unno Tatsuya,Moonjae Cho 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.1
Synbiotics synergistically favors beneficial effects of prebiotics and probiotics towards host metabolic health by modulating gut ecosystem. In this study, we sought to examine the effects of prebiotics (Schizophyllum commune derived β-(1,3/1,6)-glucan), probiotics (concoction made of eight different bacterial strains) and synbiotics (prebiotics + probiotics) on gut microbiota and its associated metabolic functions through 16S rRNA gene sequences analysis. Results showed that probiotics strains used in this study were detected more in the synbiotic and probiotic treatments, while prebiotic dietary intervention increased the total bacterial abundance and metabolisms related to host immune strengthening. Probiotics and synbiotics dietary interventions enhanced similar metabolisms relating to butanediol and s-adenosyl-l-methionine biosynthesis. Probiotics treatment also showed depleted metabolic activities related to SCFA productions, that were not depleted in prebiotics treatment. With varying differential abundance patterns and metabolic activities across the treatments, our results suggest that synbiotic treatment provide more beneficial effects over probiotics and prebiotics.
Vu Vuong,Muthuramalingam Karthika,Singh Vineet,Choi Changmin,김영미,Unno Tatsuya,Cho Moonjae 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.1
It has been proven that β-glucan produced by Schizophyllum commune has beneficial effects on obesity, obesity-associated constipation, and colitis conditions; however, the protective effect of the compound on host at basal state is yet to be investigated. C57BL/6 J mice were fed with a normal diet (ND), normal diet supplemented with 3 g/kg (BG_low), and 5 g/kg (BG_high) of β-glucan (BG) for 12 weeks. Body weight, food and water intake and fecal status were monitored weekly. Intestine was stained by Periodic acid–Schiff (PAS) and Alcian Blue to evaluate the mucin layer thickness and goblet cell population. Morphological changes in internal organs and intestinal motility were also assessed, while serum biomarkers for liver injury and glucose level were analyzed. On the other hand, fecal microbiota and associated metabolic activities were also investigated. β-glucan bulked feces, decreased fecal moisture, and proliferated goblet cells resulted in a thickened lubricating mucin layer; however, the intestinal transit rate did not increase. The abundance of beneficial bacteria was increased while the harmful strains was decreased in a dose-dependent manner by the effect of β-glucan. Specific short chain fatty acid (SCFA)-producing strains, such as Roseburia , Ruminococcus , and Bifidobacteria , were selectively increased by β-glucan. In addition, consumption of β-glucan lowered level of obesity-associated biomarkers. Schizophyllum commune β-glucan showed an insignificant change in transit rate in healthy conditions when compared with obesity, despite similar effects on increasing mucus production and bulked feces. Nonetheless, the outcomes proposed protective effects against obesity, diabetes, inflammatory bowel diseases (IBD), and constipation, in which the modification of the gut microbiota by β-glucan is the largest contributor.