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Identification of Volatile Components in Phyllanthus emblica L. and Their Antimicrobial Activity
Liu, Xiaoli,Zhao, Mouming,Luo, Wei,Yang, Bao,Jiang, Yueming The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.2
The volatile components and in vitro antimicrobial activities of Emblica (Phyllanthus emblica L.) essential oils (EOs) obtained by hydrodistillation (HD-EO) and supercritical fluid extraction (SFE-EO) were investigated. The compositions of volatile compounds in these oils were tentatively determined by gas chromatography-mass spectrometry. The antimicrobial activites of these two extracts were investigated with microbiological tests against Gram-positive and Gram-negative bacteria and three pathogenic fungi. The main components of both oils were $\beta$-caryophyllene, $\beta$-bourbonene, 1-octen-3-ol, thymol, and methyleugenol. Both essential oils showed a broad spectrum of antimicrobial activity against all the tested microorganisms. Gram-positive bacteria were more sensitive to the investigated oils than Gram-negative bacteria. SFE-EO exhibited a higher antifungal activity compared to HD-EO.
Identification of Volatile Components in Phyllanthus emblica L. and Their Antimicrobial Activity
Xiaoli Liu,Mouming Zhao,Wei Luo,Bao Yang,Yueming Jiang 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.2
The volatile components and in vitro antimicrobial activities of Emblica (Phyllanthus emblica L.) essential oils (EOs) obtained by hydrodistillation (HD-EO) and supercritical fluid extraction (SFE-EO) were investigated. The compositions of volatile compounds in these oils were tentatively determined by gas chromatography-mass spectrometry. The antimicrobial activites of these two extracts were investigated with microbiological tests against Gram-positive and Gram-negative bacteria and three pathogenic fungi. The main components of both oils were β-caryophyllene, β-bourbonene, 1-octen-3-ol, thymol, and methyleugenol. Both essential oils showed a broad spectrum of antimicrobial activity against all the tested microorganisms. Gram-positive bacteria were more sensitive to the investigated oils than Gram-negative bacteria. SFE-EO exhibited a higher antifungal activity compared to HD-EO.
Ning Liu,Yong Wang,Qiangzhong Zhao,Mouming Zhao 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.2
Diacylglycerol (DAG) was prepared viaglycerolysis of palm oil catalyzed by Lecitase Ultra (LU),a novel phospholipase from the fusion of lipase genes fromThermomyces lanuginose and phospholipase genes fromFusarium oxysporum. Glycerolysis was performed in asolvent-free system. The optimized reaction conditionswere: a glycerol/palm oil mole ratio of 7.5:1, initialsubstrate water content of 5%, substrate enzyme load of2%, reaction temperature of 40oC, and reaction time of 8 h. In a scale-up reaction, a DAG content of 59.5% in the lipidlayer was achieved. Through a two-step molecular distillation,the composition of the target product was 88.1% DAG,2.8% TAG, 9.0% MAG, and 0.1% FFA. The fatty acidcomposition of the DAG oil, determined using GC-MS,was enriched compared with the original palm oil.