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RISS 인기검색어
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- 저자 : Morad Jafari (검색결과 2 건)
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Faraz Arash Rad,Morad Jafari,Nabi Khezrinejad,Mahmoud Pouryousef Miandoab 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.6
An efficient and rapid plant regeneration system via direct organogenesis was established for felty germander(Teucrium polium L.), an endangered and valuable medicinal plant. Induction of shoot organogenesis was obtainedusing nodal explants and preconditioned intact seedlings on the MS medium supplemented with various concentrationsand combinations of plant growth regulators. The highest number of shoots (7.14/explants) was obtained from nodalexplants cultured on MS containing 2.0 mg·L-1 BAP and 0.5 mg·L-1 NAA. For preconditioning, Best response in termsof number of adventitious shoots per explant (6.85) and greatest shoot length (5.43 cm) was obtained with seedlingsperpetrated in 0.5 mg·L-1 TDZ. The rooted plantlets were acclimatized successfully in the greenhouse with a survivalrate of 100%. Significant differences (p < 0.05) were found between the greenhouse-grown tissue culture raised plantsin total flavonoid contents. Methanolic extracts of plants raised on TDZ (0.5 mg·L-1) containing medium showed thehighest amount of flavonoid contents (1.67 mg Ru·g-1 DW) and a higher antioxidant activity (52.31%) in comparisonwith the field-grown mother plant. Randomly amplified polymorphic DNA (RAPD) analysis of plants raised preconditioningprocedure revealed there were no somaclonal variations among them. Our results indicated that thecytokinin-preconditioning mediated micropropagation protocol could be used for the production of a large number oftrue-to-type plants of T. polium, which may contain higher quantities of valuable phytochemicals than those of wildplants.
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Shahabzadeh Zeynab,Darvishzadeh Reza,Mohammadi Reza,Jafari Morad,Alipour Hadi 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2
Tall fescue (Festuca arundinacea) from Poaceae family is an allohexaploid (2n=6x=42) and cross pollinated plant that is used throughout the world as a forage plant. This research was conducted to identify the genomic regions associated with 10 agro-morphological traits using 15,881 SNP markers in a diverse tall fescue germplasm. UPGMA, Structure, and PCA analyses illustrated high genetic differentiation between forage, turf, and hybrid tall fescue samples. Correcting r2 for population structure and kinship effects revealed the fact that the r2v (corrected for kinship) is much smaller than r2 (uncorrected) illustrating the necessity of removing the effect of coancestry to reduce the inflation of r2 estimates. Analysis of linkage disequilibrium revealed that the amount of SNP pairs in LD differs between sub-genomes and chromosomes and any increase in the distance among SNPs reduces the amount of SNP pairs in LD. A total of 111 and 17 marker-trait associations were identified via GLM and MLM methods for the investigated traits, respectively. Most of the significant MTAs belonged to plant height and days to anthesis. Gene ontology results revealed that 34% of MTAs were located in protein-coding regions. Most of the MTAs were located on chromosomes 1B and 1D. The MTAs for plant height and days to anthesis located within the coding regions can be utilized in genome-based breeding in tall fescue breeding programs.
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