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RISS 인기검색어
- 검색키워드
- 저자 : Monalisa Sahoo (검색결과 2 건)
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Sahoo, Nihar Ranjan,Kumar, Pushpendra,Bhushan, Bharat,Bhattacharya, T.K.,Sharma, Arjava,Dayal, Sanker,Pankaj, Prabhat Kumar,Sahoo, Monalisa Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.8
Serum lysozyme gene is one of the important genes influencing the immune system as its product can cause lysis of bacterial cell wall by cleaving the peptidoglycan layer. The present investigation on the serum lysozyme gene of Indian riverine buffalo was undertaken with the objectives to identify and characterize single nucleotide polymorphic patterns by PCR-SSCP method as well as to study the effect of different genotypes on serum lysozyme activity and somatic cell count. A total of 280 animals comprising four different famous bubaline breeds (Murrah, Mehsana, Surti and Bhadawari), spread over six different farms across the country were used for this study. A 276 bp (partial intron 2, complete exon 3 and partial intron 3) fragment of lysozyme gene was screened for polymorphism using the SSCP technique. Four genotypes namely AA, AB, BC and AC were observed, out of which BC genotype was found to be the most frequent. Among these three alleles, C allele (0.38) was most prevalent in these populations. Various SSCP allelic variants were cloned for sequencing and sequences were submitted to NCBI Genbank. From the alignment of the nucleotide sequences of various allelic variants, it was found that there were differences in 12 positions among the alleles, out of which maximum variation (at 8 places) was found in the intronic region. The allele A was closer to allele-C than allele-B. Allele B was phylogenetically equidistant from both of the other alleles. Mean lysozyme activity determined in serum samples of different animals of Murrah buffalo was $27.35{\pm}2.42\;{\mu}g$ per ml of serum, whereas the mean somatic cell count was $1.25{\pm}0.13{\times}10^5$ cells per ml of milk. The SSCP pattern-wise effects of various genotypes on lysozyme activity and SCC were analyzed. Although the mean values were apparently different in various genotypes, these differences were statistically non-significant. It can be concluded that the riverine buffaloes are sufficiently polymorphic with respect to serum lysozyme gene. The absence of AA genotype in Bhadawari breed of buffalo can be considered as a marker for breed characterization. The difference of four nucleotides in exon-3 indicates high selection pressure on the gene.
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Variability in Tropical Cyclone Climatology over North Indian Ocean during the Period 1891 to 2015
Kasturi Singh,Jagabandhu Panda,Monalisa Sahoo,M. Mohapatra 한국기상학회 2019 Asia-Pacific Journal of Atmospheric Sciences Vol.55 No.2
Tropical cyclone (TC) prediction and impact of warming environment on cyclonic activity are one of the most popular research topics. Based on sea surface temperature (SST) anomaly variation, the period 1880–2015 is divided into pre-warming (PWP; during 1880–1946) and current warming (CWP; during 1947–2015) with negative and positive anomalies respectively. Based on data availability, the period 1891–2015 is emphasized for the analysis of variability in TC climatology. The Mann-Kendall test and Sen’s slope estimation indicates a clear decreasing trend in annual TS (total storms) and CS + SCS (cyclones and severe cyclones) frequency during CWP for NIO region and particularly Bay of Bengal (BOB) at 95% confidence level. However, the TS and CS + SCS frequencies were increasing during the PWP. TC activity over southern and northern BOB is decreasing sharply during CWP. Southern sector of BOB hosts mostly severe systems and middle sector most TCs. TC activity over the eastern sector of Arabian Sea shows considerable enhancement duringCWP. An increasing SST, surface wind, mid-tropospheric relative humidity and potential evaporation factor (PEF) are helpful in the formation of intensified storms during CWP. The activities during PWP were reverse compared to that of CWP. A large temperature anomaly difference between atmosphere and Ocean also perceived to play a key role in modulating the enhanced intensity of TCs during CWP. The SST range of 27.5 °C to 29.5 °C and supportive flow field is helping to enhance the middle and upper tropospheric moisture content; eventually, resulting in increased SST, PEF and relative humidity through a possible feedback mechanism.
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