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        Utilization of Oil Palm Decanter Cake for Cellulase and Polyoses Production

        Mohamad Nafis Abdul Razak,Mohamad Faizal Ibrahim,Phang Lai Yee,Mohd Ali Hassan,Suraini Abd-Aziz 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.3

        The abundance of oil palm decanter cake (OPDC) is a problem in oil palm mills. However, this lignocellulosic biomass can be utilized for cellulase and polyoses production. The effectiveness of chemical and physical pretreatment in reducing the lignin content was studied by saccharification using a Celluclast 1.5 L and scanning electron microscope. Physicochemical pretreatment of OPDC with 1% (w/v) NaOH and autoclaving at 121oC for 20 min increased potential polyoses produced to 52.5%and removed 28.7% of the lignin content. The optimized conditions for cellulase production by a locally isolated fungus were a time of 120 h, a substrate of untreated OPDC,a spore concentration of 1 × 107 spore/mL, a temperature of 30oC, and a pH between 7.0 and 7.5. Trichoderma asperellum UPM1 produced carboxymethylcellulase (CMCase),β-glucosidase and filter paper activity (FPase) in the following concentrations: 17.35, 0.53, and 0.28 U/mL,respectively. Aspergillus fumigatus UPM2 produced the CMCase, β-glucosidase and FPase in the following amounts: 10.93, 0.76, and 0.24 U/mL. The cellulases from T. asperellum UPM1 produced 2.33 g/L of polyoses and the cellulases from A. fumigatus UPM2 produced 4.37 g/L of polyoses.

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