http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Miklos Tako ),( Alexandra Kotogan ),( Tamas Papp ),( Shine Kadaikunnan ),( Naiyf S. Alharbi ),( Csaba Vagvolgyi ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.2
Rhizomucor miehei NRRL 5282 and Rhizopus oryzae NRRL 1526 can produce lipases with high synthetic activities in wheat bran-based solid-state culture. In this study, the purification and biochemical characterization of the lipolytic activities of these lipases are presented. SDSPAGE indicated a molecular mass of about 55 and 35 kDa for the purified R. miehei and Rh. oryzae enzymes, respectively. p-Nitrophenyl palmitate (pNPP) hydrolysis was maximal at 40°C and pH 7.0 for the R. miehei lipase, and at 30°C and pH 5.2 for the Rh. oryzae enzyme. The enzymes showed almost equal affinity to pNPP, but the V<sub>max</sub> of the Rh. oryzae lipase was about 1.13 times higher than that determined for R. miehei using the same substrate. For both enzymes, a dramatic loss of activity was observed in the presence of 5 mM Hg<sup>2+</sup>, Zn<sup>2+</sup>, or Mn<sup>2+</sup>, 10 mM N-bromosuccinimide or sodium dodecyl sulfate, and 5-10% (v/v) of hexanol or butanol. At the same time, they proved to be extraordinarily stable in the presence of nhexane, cyclohexane, n-heptane, and isooctane. Moreover, isopentanol up to 10% (v/v) and propionic acid in 1 mM concentrations increased the pNPP hydrolyzing activity of R. miehei lipase. Both enzymes had 1,3-regioselectivity, and efficiently hydrolyzed p-nitrophenyl (pNP) esters with C8-C16 acids, exhibiting maximum activity towards pNP-caprylate (R. miehei) and pNP-dodecanoate (Rh. oryzae). The purified lipases are promising candidates for various biotechnological applications.
Hybrid Fuzzy Adaptive Control of LEGO Robots
Vaseak, Jan,Miklos, Marian Korean Institute of Intelligent Systems 2002 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.2 No.1
The main drawback of “classical”fuzzy systems is the inability to design and maintain their database. To overcome this disadvantage many types of extensions adding the adaptivity property to those systems were designed. This paper deals with one of them a new hybrid adaptation structure, called gradient-incremental adaptive fuzzy controller connecting gradient-descent methods with the so-called self-organizing fuzzy logic controller designed by Procyk and Mamdani. The aim is to incorporate the advantages of both Principles. This controller was implemented and tested on the system of LEGO robots. The results and comparison to a ‘classical’(non-adaptive) fuzzy controller designed by a human operator are also shown here.
Aspirin resistance as cardiovascular risk after kidney transplantation
Barbara Sandor,Adam Varga,Miklos Rabai,Andras Toth,Judit Papp,Kalman Toth,Peter Szakaly 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.2
International surveys have shown that the leading cause of death after kidney transplantation has cardiovascularorigin with a prevalence of 35-40%. As a preventive strategy these patients receive aspirin (ASA)therapy, even though their rate of aspirin resistance is still unknown. In our study, platelet aggregation measurementswere performed between 2009 and 2012 investigating the laboratory effect of low-dose aspirin(100 mg) treatment using a CARAT TX4 optical aggregometer. ASA therapy was considered clinically effectivein case of low (i.e., below 40%) epinephrine-induced (10 μM) platelet aggregation index. Rate of aspirinresistance, morbidity and mortality data of kidney transplanted patients (n = 255, mean age: 49 ± 12 years)were compared to a patient population with cardio- and cerebrovascular diseases (n = 346, mean age: 52.6 ± 11years). Rate of aspirin resistance was significantly higher in the renal transplantation group (RT) compared tothe positive control group (PC) (35.9% vs. 25.6%, p < 0.002). Morbidity analysis demonstrated significantlyhigher incidence of myocardial infarction, hypertension and diabetes mellitus in the RT group (p < 0.05). Thesubgroup analysis revealed significantly higher incidence of infarction and stroke in the ASA resistant RTgroup compared to the RT patients without ASA resistance (p < 0.05). Furthermore, the incidence of myocardialinfarction and hypertension was significantly higher in the non-resistant RT group than in the groupof PC patients without ASA resistance (p < 0.05). These results may suggest that the elevated rate of aspirinresistance contributes to the high cardiovascular mortality after kidney transplantation.
Mihalka, Virag,Fari, Miklos,Szasz, Attila,Balazs, Ervin,Nagy, Istvan The Korean Society of Plant Biotechnology 2000 Plant molecular biology and biotechnology research Vol.2 No.3
An Efficient in vitro regeneration system and an optimized Agrobacterium mediated transformation protocol are described, based on the use of young seedling cotyledons of Capsicum annuum L. Optimal regeneration efficiency can be obtained by cultivating cotyledon explants on media containing 4 mg/L benzyladenine and 0.1 mg/L indolacetic acid. The effect of antibiotics used to eliminate Agrobacteria, as well as the toxic level of some generally used selection agents (kanamycin, geneticin, hygromycin, phosphinotricin and methotrexate) in regenerating pepper tissues were determined. To enable the comparison of different selection markers in identical vector background, a set of binary vectors containing the marker genes for NPTII, HPT, DHFR and BAR respectively, as well as the CaMV 35S promoter/enhancer-GUS chimaeric gene was constructed and introduced into four different Agrobacterium host strains.
Aspirin resistance as cardiovascular risk after kidney transplantation
Sandor, Barbara,Varga, Adam,Rabai, Miklos,Toth, Andras,Papp, Judit,Toth, Kalman,Szakaly, Peter 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.2
International surveys have shown that the leading cause of death after kidney transplantation has cardiovascular origin with a prevalence of 35-40%. As a preventive strategy these patients receive aspirin (ASA) therapy, even though their rate of aspirin resistance is still unknown. In our study, platelet aggregation measurements were performed between 2009 and 2012 investigating the laboratory effect of low-dose aspirin (100 mg) treatment using a CARAT TX4 optical aggregometer. ASA therapy was considered clinically effective in case of low (i.e., below 40%) epinephrine-induced ($10{\mu}M$) platelet aggregation index. Rate of aspirin resistance, morbidity and mortality data of kidney transplanted patients (n = 255, mean age: $49{\pm}12$ years) were compared to a patient population with cardio- and cerebrovascular diseases (n = 346, mean age: $52.6{\pm}11$ years). Rate of aspirin resistance was significantly higher in the renal transplantation group (RT) compared to the positive control group (PC) (35.9% vs. 25.6%, p < 0.002). Morbidity analysis demonstrated significantly higher incidence of myocardial infarction, hypertension and diabetes mellitus in the RT group (p < 0.05). The subgroup analysis revealed significantly higher incidence of infarction and stroke in the ASA resistant RT group compared to the RT patients without ASA resistance (p < 0.05). Furthermore, the incidence of myocardial infarction and hypertension was significantly higher in the non-resistant RT group than in the group of PC patients without ASA resistance (p < 0.05). These results may suggest that the elevated rate of aspirin resistance contributes to the high cardiovascular mortality after kidney transplantation.