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RISS 인기검색어
- 검색키워드
- 저자 : Merit Ayevbuomwan (검색결과 3 건)
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Merit E. Ayevbuomwan,Olusola O. Elekofehinti,Francis A. Obuseh,Ehimwenma S. Omoregie 경희대학교 융합한의과학연구소 2021 Oriental Pharmacy and Experimental Medicine Vol.21 No.4
Detarium microcarpum is a legume tree of tropical Africa, widely known for its ethno-medicinal potentials. Previous studies have reported its rich contents of phytochemicals which could be linked to the bioactive principle of the plant. The in vitro antisickling properties of D. microcarpum stem bark was evaluated using blood samples obtained from thirty-fve confrmed sickle cell disease patients using standard microscopic techniques. The research outcome showed that D. microcarpum extract inhibited sickling of red blood cells with a laudable mean diference compared to p-hydrozybenzoic acid (reference antisickling drug) which is a pure compound. To further expounds it’s mechanism of action, six compounds previously isolated from D. microcarpum stem bark and fruits was docked into the active sites of deoxy-hemoglobin (PDB ID: IGZX) and 2, 3-bisphosphoglycerate mutase (PDB ID: 1T8P) using Autodock Vina algorithm. Results from the in silico studies showed that oxokolavenic acid, 5α, 8α -2-oxokalavenic acid and Copalic acid exhibited the most favorable ligand–protein molecular interaction with deoxy-hemoglobin and 2,3-bisphosphoglycerate mutase when compared with the reference antisickling drug, hydroxyurea. The interaction of these compounds with deoxy-hemoglobin could alter hemoglobin conformation, favoring the relaxed state, thereby delaying/inhibiting hemoglobin polymerization and sickling. Their interactions with 2, 3-bisphosphoglycerate mutase could inhibit 2, 3-bisphosphoglycerate synthesis, thus delaying enhanced delivery of oxygen to tissues which is twice the case seen in sickle cell anemia. This clearly shows the possible mechanism of action of D. microcarpum and further substantiates the antisickling potential of these compounds derived from D. microcarpum leaves and fruits.
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Ehigbai I. Oikeh,Merit Ayevbuomwan,Francis Irabor,Aiemere O. Oikeh,Faith E. Oviasogie,Ehimwenma S. Omoregie 한국식품영양과학회 2020 Preventive Nutrition and Food Science Vol.25 No.3
The seeds of Citrus sinensis (L.) Osbeck (sweet orange) are waste products usually discarded. They may however contain phytochemicals that have potent bioactivities. In this study, the phenolic content, and antioxidant and antimicrobial activities of oil and non-oil (solid) extracts of C. sinensis seeds were evaluated using standard protocols. The seed oil contained significantly (P>0.05) higher contents of total phenol and total flavonoid when compared to the solid extract. However, the non-oil extract contained significantly (P<0.05) higher tannin contents than the seed oil. Ferric reducing antioxidant potential was not significantly different between both extracts. The antimicrobial activities of both extracts revealed that the seed oil possesses better antibacterial activities compared to the non-oil extract. The antifungal test revealed that the seed oil significantly inhibited the growth of Candida albicans (20 ㎜ zone of inhibition at a concentration of 200 ㎍/mL), however, it did not inhibit the growth of Aspergillus niger and Penicillum sp. The minimum inhibitory concentration values against the bacterial and fungal strains were similar for both extracts in the range of 50∼100 ㎍/mL. Minimum bactericidal concentration and minimum fungicidal concentration values ranged from 100∼200 ㎍/mL for both extracts. The results in this study indicate that C. sinensis seed oil and non-oil extracts possess antioxidant, and antibacterial and antifungal properties that may be differentially exploited in the development of antimicrobial agents.
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Osamudiamen Ebohon,Francis Irabor,Ayevbuomwan Merit Esewi,Omoregie Ehimwenma Sheena 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.2
Oxidative stress, already implicated in malaria infection has been identified as one of the major contributors to the pathophysiology of malaria. This study was aimed at evaluating the effects of methanol extracts of Tetrorchidium didymostemon leaf and stem bark on Plasmodium berghei induced oxidative stress in the liver, spleen and brain of mice. P. berghei-infected mice were sacrificed on day 5 of the experiment after four days of consecutive administration of T. didymostemon extracts (250 and 500 mg/kg body weight). Thereafter, biochemical analysis and histopathological examination were carried out. The leaf extract had a significantly higher (P < 0.05) ability to reduce parasite induced alterations of antioxidant enzyme activities compared with the stem bark extract. Malondialdehyde level was significantly higher (P < 0.05) while glutathione peroxidase and catalase activities were lower in the negative control (infected mice, without treatment) relative to the treated groups. The leaf extract at 500 mg/kg body weight had a higher ability to ameliorate changes in oxidative stress and reduce hepatic injury induced by P. berghei in comparison with the other doses. The leaf extract (500 mg/kg) was able to reduce significantly hepatomegaly induced by P. berghei. Similarly, histopathological observation of the organs (liver and spleen) shows relative reversal of the cellular and morphological alteration induced by P. berghei infection following leaf extract administration. Our study suggests that treatment of P. berghei infected mice with T. didymostemon leaf extract during early infection reduces oxidative stress by preventing lipid peroxidation and normalizing glutathione peroxidase and catalase activities.
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