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- 저자 : Meng-Shiou Lee (검색결과 2 건)
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Low-Cost Camera Based Laser Power Monitoring and Stabilizing for Micro-Hole Drilling
Chien-Fang Ding,Meng-Shiou Lee,Kuan-Ming Li 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.9
In this study, a laser power monitoring and stabilizing system for micro-hole drilling based on optical outputs of a CMOS sensor is presented. The correlation between the laser power and the average brightness of the beam spots on the images was investigated. The estimated laser power was used to build an on-line closed loop control of micro-hole drilling. Experimental results showed that the shortest response time of the laser monitoring system was only 30 ms, which was much faster than a thermopile power meter. The average measuring error was 3%, compared with thermopile power meter. In the PCB drilling experiments of 30 kHz pulse repetition frequency, the PID controller could compensate the power disturbances from 38.4% to 1.8%, and the aspect ratio from 20% to 5%. This study demonstrates the feasibility to develop a low-cost laser power monitoring and stabilizing system in laser micromachining processes.
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Yi-Yang Lien,Chi-Hung Huang,Fang-Chun Sun,Shyang-Chwen Sheu,Tsung-Chi Lu,Meng-Shiunn Lee,Shu-Chin Hsueh,Hsi-Jien Chen,Meng-Shiou Lee 대한수의학회 2012 JOURNAL OF VETERINARY SCIENCE Vol.13 No.1
Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd129, was cloned into an Escherichia (E.) coli expression vector. After isopropyl-b-D-thiogalactopyronoside induction, VP1Nd129 protein was shown to be successfully expressed in the E. coli. By performing an enzyme-linked immunoabsorbent assay using two coating antigens, purified VP1Nd129 and CAV-infected liver tissue lysate, E3 monoclonal antibody (mAb) was found to have higher reactivity against VP1 protein than the other positive clones according to the result of limiting dilution method from 64 clones. Using immunohistochemistry, the presence of the VP1-specific mAb, E3, was confirmed using CAV- infected liver and thymus tissues as positive-infected samples. Additionally, CAV particle purification was also performed using an immunoaffinity column containing E3 mAb. The monoclonal E3 mAb developed in this study will not only be very useful for detecting CAV infection and performing histopathology studies of infected chickens, but may also be used to purify CAV particles in the future.
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