RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      선택해제
      • 좁혀본 항목 보기순서

        • 원문유무
        • 원문제공처
        • 등재정보
        • 학술지명
        • 주제분류
        • 발행연도
        • 작성언어
        • 저자
          펼치기
      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재

        Somatic Embryogenesis in Cyclamen with Two Explants and Combinations of Plant Growth Regulators

        Neda Jalali,Roohangiz Naderi,Mesbah Babalar,Masoud Mirmasoumi 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.5

        Cyclamen persicum Mill. (Myrsinaceae ) is one of the most important winter pot flowers in the world. It is commercially propagated via F₁-hybrid seeds. Somatic embryogenesis is an efficient method of micropropagation and biotechnological tools for genetic improvement in cyclamen. The effect of initial explants type (leaf and petiole) on callus growth and somatic embryo were studied. Somatic embryos were cultured on modified Murashige and Skoog (MS) medium. Also embryogenic callus was induced on several media containing 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (Kin). The greatest amount of callus was observed in two explants on media containing 1 ㎎?L<SUP>-1</SUP> 2,4-D + 0.5 ㎎?L<SUP>-1</SUP> Kin. The optimal responses of somatic emberyogenesis were recorded on media enriched with 4 ㎎?L<SUP>-1</SUP> 2,4-D, 0.1 ㎎?L<SUP>-1</SUP> Kin and leaf explants. These results were obtained from adult plants.

      • KCI등재

        Optimization of Walnut Tissue Culture Medium Using Seed Composition

        Elahe Najafian Ashrafi,Kourosh Vahdati,Hassan Ebrahimzadeh,Masoud Mirmasoumi,Naser Lotfi 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.2

        Micropropagation of walnut has been partially limited to the lack of an appropriate culture medium. We hypothesized that the minerals in proportions similar to those found in the walnut seeds could provide an optimum medium for shoot micropropagation. The mineral composition of five walnut seeds as ‘Serr’, ‘Pedro’, ‘Lara’, ‘Hartley’ and ‘Rond e Montignac’ were analyzed by Inductive Couple Plasma-Omission Spectroscopy (ICP-OES). In addition, to study the effect of Cu and myo-inositol on shoot length and rooting rate of explants, these factors were examined. Concentrations of mineral compositions of walnut seeds were several times of those in DKW (Driver and Kuniyuki Walnut medium). Based on minimum concentration of seeds, two new media (as ×1.5 and ×2 DKW) were formulated. Growth of explants of ’Hartley‘, ‘Sunland’ and ‘Vina’ cultivars on two new media was compared to those cultured on DKW medium. The explants cultured on ×2 DKW and ×1.5 DKW formed shoots having green-colored leaves but produced callus similar to those cultured on DKW medium. Better growth was observed on ×1.5 DKW medium. Potential multiplication rate was different among the cultivars and ‘Vina’ had the maximum stem length and number of auxiliary buds on ×1.5 DKW. The results of the second experiment indicated that number of auxiliary buds per shoot and stem length were enhanced in higher levels of Cu and myo-inositol. In vitro rooting percentage for ‘Sunland’, ‘Hartley’ and ‘Vina’ were 50, 31 and 16% in the DKW medium, respectively but decreased to 5-17% in two modified media (×1.5 and ×2 DKW). A better rooting (up to 70%) was obtained when shoots were placed in higher levels of Cu and myo-inositol (CM2). The results suggested that some of the walnut cultivars need to grow on higher mineral concentrations of DKW medium. In addition, the higher levels of Cu and myo-inositol would be affective on growth rate and rooting percentage of walnut explants.

      • KCI등재

        Using hairy roots for production of secondary metabolites in Artemisia

        Zahra Mottaki,Maryam Rezayian,Vahid Niknam,Hassan Ebrahimzadeh,Masoud Mirmasoumi 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3

        Secondary metabolites are a group of chemical compounds in plants which have various functions and applications in different industries such as leather and pharmaceutical products. Artemisia is a genus of important vascular plants which contains many species. There are many chemical compounds in Artemisia which are still unknown. Additionally, hairy root culture in plants is considered to be one of the new techniques applied to production of secondary metabolites which denotes the vast application of this technique. Hairy root culture as a differentiated tissue has been under consideration for many researchers which is because of its genetic stabilization; its rapid growth in a hormone-free medium is also very important. The current study is conducted with the objective of hairy root induction in explant parts of some Artemisia species with various strains of Agrobacterium rhizogenes. The results show that total protein content and enzyme activity during hairy root induction has an ascending procedure. Phenol and flavonoid content have shown considerable increase. The sterol and essential oil have also shown interesting consistency towards each other. To the best of our knowledge, this is the first report of production secondary metabolites and antioxidant activities by hairy root-derived Artemisia. This study may open possibilities for production of the pharmaceutically high-value secondary metabolites.

      연관 검색어 추천

      이 검색어로 많이 본 자료

      활용도 높은 자료

      해외이동버튼