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- 저자 : María Salazar (검색결과 3 건)
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Julián David Salazar-Gutiérrez,Andrés Castelblanco,María Ximena Rodríguez-Bocanegrab,Wilson Teran,Adriana Sáenz-Aponte 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4
Entomopathogenic nematodes are key players for insect pest control and constitute an environmentally friendly alternative for crop protection. The insecticidal activity of the family Heterorhabditidae relies on a tight symbiotic relationship with enterobacteria of the genus Photorhabdus, where the bacterial contribution towards the death of the host has been highlighted. In the present work, we report the identification and pathogenic characterization of Photorhabdus luminescens strain SL0708, which is the natural symbiont of Heterorhabditis indica SL0708. We evaluated the pathogenicity of whole bacterial cells and acellular extracts against both Spodoptera frugiperda larvae and Galleria mellonella. Phylogenetic analyses using a polygenic sequencing approach assigned the bacterial strain to Photorhabdus luminescens subsp. akhurstii and bioassays showed it is highly pathogenic for both insects. After 48 h of treatment with 1× 103 −1 × 104 CFU/larva, 100% mortality was attained. Furthermore, when intra- or extracellular bacterial extracts were injected into G. mellonella, a cumulative percent mortality of 63% and 100% was respectively obtained after 72 h. In contrast, a 10% and 93% mortality was achieved for S. frugiperda with intra and extracellular extracts, respectively highlighting the role of extracellular factors in pathogenicity. We detected extracellular activities potentially accounting for the high pathogenicity observed and these included; proteases, esterases, ureases, hemolysins and siderophores. Interestingly, S. frugiperda was more susceptible to P. luminescens SL0708 cells than G. mellonella, which contrasted to its higher resistance to H. indica SL0708 nematodes, which suggests that EPN biological control potential should also be evaluated based on bacterial symbiont pathogenicity
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Miguel Fdo. Salazar,María del Rocío Estrada Hernández,Erick Gómez Apo,Laura G. Chávez Macías,Carlos Alfonso Rodríguez Álvarez 대한병리학회 2015 Journal of Pathology and Translational Medicine Vol.49 No.5
We report a unique case of a 4-year-old girl with an intriguing fibrohistiocytic tumour. Magnetic resonance imaging scans showed a dural mass of variegated intensity compressing the left occipital pole and apparently extending toward the superior sagittal sinus. Grossly, the cut surface of the surgical specimen was yellow, pale, and soft with reddish kernel-like crusts. Histologically, the yellow areas resembled cholesterol granulomas with widespread coagulative necrosis, cholesterol clefts, powdery calcification, foreign body-type giant cells, and foamy macrophages, while the scattered red spots contained numerous multinucleated giant cells of foreign-body and Touton types, the former with amphophilic to slightly eosinophilic cytoplasm. Immunoperoxidase reactions confirmed the expression of histiocytic markers and vimentin. As far as we know, no tumour displaying these peculiar morphological features has yet been described.
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Luis D´Marco,Juan Salazar,Marie Cortez,María Salazar,Marjorie Wettel,Marcos Lima-Martínez,Edward Rojas,Willy Roque,Valmore Bermúdez 대한신장학회 2019 Kidney Research and Clinical Practice Vol.38 No.3
Background: Adipose tissue accumulation in specific body compartments has been associated with diabetes, hypertension and dyslipidemia. Perirenal fat (PRF) may lead to have direct lipotoxic effects on renal function and intrarenal hydrostatic pressure. This study was undertaken to explore the association of PRF with cardiovascular risk factors and different stages of chronic kidney disease (CKD). Methods: We studied 103 patients with CKD of different stages (1 to 5). PRF was measured by B-mode renal ultrasonography in the distal third between the cortex and the hepatic border and/or spleen. Results: The PRF thickness was greater in CKD patients with impaired fasting glucose than in those with normal glucose levels (1.10 ± 0.40 cm vs. 0.85 ± 0.39 cm, P < 0.01). Patients in CKD stages 4 and 5 (glomerular filtration rate [GFR] < 30 mL/min/1.73 m2) had the highest PRF thickness. Serum triglyceride levels correlated positively with the PRF thickness; the PRF thickness was greater in patients with triglyceride levels ≥ 150 mg/dL (1.09 ± 0.40 cm vs. 0.86 ± 0.36 cm, P < 0.01). In patients with a GFR < 60 mL/min/1.73 m2, uric acid levels correlated positively with the PRF thickness (P < 0.05). Conclusion: In CKD patients, the PRF thickness correlated significantly with metabolic risk factors that could affect kidney function.
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