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제브라피쉬 배아의 다양한 단계에서 알로에 추출물의 기형유도 능력 평가
Manigandan Krishnan,Jai Heon Lee,Sun Chul Kang 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Obvious applications of aloe (Aloe vera) and their rapid intake make it crucial to evaluate their biological impacts to aquatic organisms. Methods and Results : In this study, we investigated the possible effects of aloe extract on zebrafish embryos (Danio rerio) by assessing teratogenic properties including survival rate, hatching success, heart rate and morphological changes for 96hpf (hours post fertilization). We noticed that exposure to aloe extracts (0.1, 10, 50, 100 and 150 μl/ml in embryo medium) to zebrafish embryos showed no alterations in survival and morphological changes when compared to control embryos. In contrast, embryos exposed to 150 μl/ml of aloe extract shows delayed hatching rate at 72hpf when compared to lower concentrations. Similarly, aloe extract at 150 μl/ml exposed embryos elicited significantly reduced heart rate (132 ± 1 beats/min) at 48hpf when compare to control embryos. Conclusion : Based on the above findings, we speculate the teratogenic effect of aloe extract on zebrafish embryos.
Anti-Endotoxin 9-Meric Peptide with Therapeutic Potential for the Treatment of Endotoxemia
( Manigandan Krishnan ),( Joonhyeok Choi ),( Sungjae Choi ),( Yangmee Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.1
Inflammatory reactions activated by lipopolysaccharide (LPS) of gram-negative bacteria can lead to severe septic shock. With the recent emergence of multidrug-resistant gram-negative bacteria and a lack of efficient ways to treat resulting infections, there is a need to develop novel anti-endotoxin agents. Antimicrobial peptides have been noticed as potential therapeutic molecules for bacterial infection and as candidates for new antibiotic drugs. We previously designed the 9-meric antimicrobial peptide Pro9-3 and it showed high antimicrobial activity against gram-negative bacteria. Here, to further examine its potency as an anti-endotoxin agent, we examined the antiendotoxin activities of Pro9-3 and elucidated its mechanism of action. We performed a dye-leakage experiment and BODIPY-TR cadaverine and limulus amebocyte lysate assays for Pro9-3 as well as its lysine-substituted analogue and their enantiomers. The results confirmed that Pro9-3 targets the bacterial membrane and the arginine residues play key roles in its antimicrobial activity. Pro9-3 showed excellent LPS-neutralizing activity and LPS-binding properties, which were superior to those of other peptides. Saturation transfer difference-nuclear magnetic resonance experiments to explore the interaction between LPS and Pro9-3 revealed that Trp3 and Tlr7 in Pro9-3 are critical for attracting Pro9-3 to the LPS in the gram-negative bacterial membrane. Moreover, the anti-septic effect of Pro9-3 in vivo was investigated using an LPS-induced endotoxemia mouse model, demonstrating its dual activities: antibacterial activity against gram-negative bacteria and immunosuppressive effect preventing LPS-induced endotoxemia. Collectively, these results confirmed the therapeutic potential of Pro9-3 against infection of gram-negative bacteria.
제브라피쉬 배아발생에 관한 식품첨가제 티몰의 기형발생 효과
마니간단 ( Manigandan Krishnan ),강선철 ( Sun Chul Kang ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-
Biological process of redox reaction is an integral mechanism of toxicities, contributing to severe pathological diseases. Thymol is a well-accepted food additive agent as well as antiseptic; however, its beneficial applications have toxic effects on zebrafish developmental stages. To explore the underlying mode of action of thymol (THY), we investigated the potential impact of THY on zebrafish embryos, with special attention on morphological and ROS biogenesis. In this study, we exposed the zebrafish embryos to 5, 50 and 250 μM of THY for 96 hours post fertilization (hpf). Noticeable teratogenic effects were observed upon assessing the survival rate (LC50=40μM), hatching process, and cardiac functions, thereby verifying the toxic effect of THY on zebrafish embryos. Furthermore, we analyzed the effect of THY on the activities of morphological, JC1 and Casp-3 immunofluorescence staining, followed by expressional analysis of antioxidant (superoxide dismutase (SOD), catalase (CAT) and apoptotic markers in zebrafish. As expected, we noticed a significant modulatory effect on the above-mentioned activities. Collectively, our findings suggest that ROS might be the prime mediator responsible for THY-induced oxidative damage, thereby affecting the cellular defense mechanism and apoptotic events in zebrafish embryos.
Giribabu, Krishnan,Oh, Seo Yeong,Suresh, Ranganathan,Kumar, Sivakumar Praveen,Manigandan, Ramadoss,Munusamy, Settu,Gnanamoorthy, Govindhan,Kim, Jun Yeong,Huh, Yun Suk,Narayanan, Vengidusamy Springer-Verlag 2016 Mikrochimica acta Vol.183 No.8
<P>We describe the preparation of a nanohybrid consisting of nitrogen doped reduced graphene oxide and CuS nanoparticles (N-rGO/CuS) by in-situ microwave irradiation at weight ratios of 25/75, 50/50, and 75/25. The resulting nanohybrids were characterized by X-ray diffraction, X-ray photoelectron spectroscopy, FTIR, spectroscopy, scanning electron and transmission electron microscopy, electrochemically by cyclic voltammetry and electrochemical impedance analysis. It is shown that the CuS nanoparticles are evenly decorated onto the N-rGO surface. The nanohybrids was placed on glassy carbon electrode (GCE) where they showed electro-reductive activity towards picric acid, typically at working voltages between -0.2 and -0.8 V (vs. SCE). Effects of pH value and scan rate were evaluated, and it is shown that two electrons are involved in electro-reduction. The detection limits of the GCE modified with various N-rGO/CuS hybrids (with 25/75, 50/50, and 75/25 wt%) are 6.2, 3.2, and 0.069 mu M respectively. The method demonstrates its applicability in sensing of picric acid with good reproducibility.</P>