http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kumar, Arthikala Manoj,Sreevathsa, Rohini,Reddy, Kalpana Nanja,Ganesh, Prasa Trichy,Udayakumar, Makarla 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.2
Agrobacterium tumefaciens mediated in planta transformation protocol was developed for castor, Ricinus communis. Two-day-old seedlings were infected with Agrobacterium strain EHA105/pBinBt8 harboring cry1AcF and established in the greenhouse. Screening the $T_1$ generation seedlings on 300 mg $L^{-1}$ kanamycin identified the putative transformants. Molecular and expression analysis confirmed the transgenic nature and identified high-expressing plants. Western blot analysis confirmed the co-integration of the nptII gene in the selected transgenic plants. Bioassay against Spodoptera litura corroborated with high expression and identified five promising effective lines. Analysis of the $T_2$ generation plants proved the stability of the transgene indicating the feasibility of the method.
Arthikala Manoj Kumar,Rohini Sreevathsa,Kalpana Nanja Reddy,Prasa Trichy Ganesh,Makarla Udayakumar 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.2
Agrobacterium tumefaciens mediated in planta transformation protocol was developed for castor, Ricinus communis. Two-day-old seedlings were infected with Agrobacterium strain EHA105/pBinBt8 harboring cry1AcF and established in the greenhouse. Screening the T1 generation seedlings on 300 mg L^(-1) kanamycin identified the putative transformants. Molecular and expression analysis confirmed the transgenic nature and identified high-expressing plants. Western blot analysis confirmed the co-integration of the nptII gene in the selected transgenic plants. Bioassay against Spodoptera litura corroborated with high expression and identified five promising effective lines. Analysis of the T2 generation plants proved the stability of the transgene indicating the feasibility of the method.
Yamunarani Ramegowda,Ramegowda Venkategowda,Pavithra Jagadish,Geetha Govind,Rajashekar-Reddy Hanumanthareddy,Udayakumar Makarla,Shankar Ambarahalli Guligowda 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
Zinc (Zn) deficiency is very widespread bothfrom the plant and human nutrition perspective. One of theapproaches to improve Zn in crop plants is by overexpressionof Zn transporters such that plants can uptakeand accumulate additional Zn added to soil. The ZIP familytransporters generally contribute to Zn homeostasis inplants by regulating Zn transport into the cell. We overexpressedOsZIP1 in finger millet and model plant tobaccounder the control of constitutive (35S) and endospermspecific(Bx17) promoters to study the improvement in Znaccumulation. The transcript analysis revealed the inductionof ZIP1 in leaf and root tissue under Zn deprivation infinger millet cultivars. Ectopic expression of OsZIP1 intobacco under CaMV35S (n35S) and Bx17 (nBx17)improved seed Zn concentration compared to untransformedwild-type plants. In addition, we successfullydeveloped finger millet transgenic plants, f35S and fBx17,expressing the OsZIP1 under 35S (f35S) and Bx17 (fBx17)promoter, respectively. The transgenic plants accumulatedsignificantly higher Zn in seeds compared to untransformedwild-type finger millet plants. Plants expressing the geneunder Bx17 promoter accumulated more Zn in seed than35S plants. Apart from Zn, finger millet transgenic plantsalso showed increased Mn content in seeds. Our resultsshow the involvement of OsZIP1 in improving Zn concentrationin tobacco and finger millet. Using endospermspecificpromoter, it is possible to improve the seed Znconcentration in the edible part of cereals.
Ramegowda, Yamunarani,Venkategowda, Ramegowda,Jagadish, Pavithra,Govind, Geetha,Hanumanthareddy, Rajashekar-Reddy,Makarla, Udayakumar,Guligowda, Shankar Ambarahalli 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
Zinc (Zn) deficiency is very widespread both from the plant and human nutrition perspective. One of the approaches to improve Zn in crop plants is by over-expression of Zn transporters such that plants can uptake and accumulate additional Zn added to soil. The ZIP family transporters generally contribute to Zn homeostasis in plants by regulating Zn transport into the cell. We over-expressed OsZIP1 in finger millet and model plant tobacco under the control of constitutive (35S) and endospermspecific (Bx17) promoters to study the improvement in Zn accumulation. The transcript analysis revealed the induction of ZIP1 in leaf and root tissue under Zn deprivation in finger millet cultivars. Ectopic expression of OsZIP1 in tobacco under CaMV35S (n35S) and Bx17 ($nB{\times}17$) improved seed Zn concentration compared to untransformed wild-type plants. In addition, we successfully developed finger millet transgenic plants, f35S and $fB{\times}17$, expressing the OsZIP1 under 35S (f35S) and Bx17 ($fB{\times}17$) promoter, respectively. The transgenic plants accumulated significantly higher Zn in seeds compared to untransformed wild-type finger millet plants. Plants expressing the gene under Bx17 promoter accumulated more Zn in seed than 35S plants. Apart from Zn, finger millet transgenic plants also showed increased Mn content in seeds. Our results show the involvement of OsZIP1 in improving Zn concentration in tobacco and finger millet. Using endosperm-specific promoter, it is possible to improve the seed Zn concentration in the edible part of cereals.