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( Aritra Roy Choudhury ),( Mak Chanratana ),( Shamim Ahmed ),( Md Abdul Halim ),사동민 ( Tongmin Sa ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-
The formulation of plant growth promoting bacteria as a potential bioinoculant to combat against various biotic and abiotic stresses have been shown to be beneficial for both plants and environment. The industrial application and market value of bioinoculants compared to conventional fertilizers are not high. The major limitations are low quality and the lack of applicable formulation for bioinoculants. These bioinoculants need proper carrier materials for delivery into the agricultural lands for their enhanced functionality, survivability and increase in their shelf life during storage. This study was conducted to compare the plant growth promoting effects of immobilized M. oryzae CBMB20 in chitosan and alginate beads on tomato plant under greenhouse conditions. The immobilized M. oryzae CBMB20 in various formulations were c ted in tomato (Solanum lycopersicum) seeds using 1% (w/v) carboxymethyl cellulose as an adhesive. Various physiological properties of plants such as plant length, dry weight of root and shoot, etc were measured after 15 days of transplanting. The survivability of M. oryzae CBMB20 and degradation of carrier material in soil was checked in regular intervals during the growing stage of tomato plant. The application of chitosan immobilized M. oryzae CBMB20 indicated up to 1.3 fold increase in the shoot and root lengths as well as plant dry weight of tomato plant under greenhouse condition compared to other treatments. The chitosan treated tomato seeds showed significant increase in germination percentage and chlorophyll content. Immobilized M. oryzae CBMB20 showed 1.5 fold higher survivability in tomato rhizosphere soil compared to free cells with around 4.35 log CFU g<sup>-1</sup> of soil for chitosan at 21 DAS. The alginate beads got fully degraded whereas there was partial degradation of chitosan beads after 30 days. Chitosan was able to keep a considerable survivability of M. oryzae CBMB20 for over 20 days. These observations confer that chitosan is more suitable carrier material for immobilizing M. oryzae CBMB20. Furthermore, such formulation holds good promise to be utilized with other PGPB strains and crops for potential large scale application in agricultural fields.
Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants
Gopal Selvakumar,Kiyoon Kim,Denver Walitang,Mak Chanratana,Yeongyeong Kang,Bongnam Chung,Tongmin Sa 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.5
Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.
김용헌 ( Md Abdul Halim ),사동민 ( Mak Chanratana ),( Shamim Ahmed ),( Yongheon Kim ),( Tongmin Sa ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-
Successful colonization of bio-inoculants face hostile environmental conditions like salinity in the rhizosphere of plant. The survivability and adaptation under stress is a major concern for bio-inoculants. Thus, the current study were focused on survivability improvement of the chitosan aggregated Methylobacterium oryzae CBMB20 and pant growth promotion (PGP) of tomato plant under salt stress. At different temperature (4, 30, 40 and 50℃) the capability of the bio-inoculant with either liquid or chitosan based formulation was observed for 3 months of storage and used to examine the effect on tomato plant under salt stress. Furthermore, the poly-β-hydroxybuterate (PHB) content, exopolysaccharide (EPS) production, biofilm formation, and microbial cell hydrophobicity as physiological parameter were studied. It was revealed that the aggregated exposed statistically significant over non-aggregated bioinoculants. In addition, to tolerate UV, heat, desiccation, low temperature, starvation, and H2O2 potentiality were higher for the aggregated M. oryzae CBMB20 than other. Particularly, chitosan immobilized aggregated M. oryzae CBMB20 exhibit 4 to 30℃ optimum storage temperature and 24.67, 36.65 and 59.53% reduction in cfu counts after 90 days of storage at temperatures 4, 30 and 40℃), respectively. Moreover, chitosan immobilized aggregated M. oryzae CBMB20 evinced by 1.9, 1.6 and 1.9-times seed germination, seedling vigor index and plant dry weight, consequently under salt. Hence, chitosan boost the lodging the aggregated bacterial cells that prompt the bio-inoculants survivability and salt stress adeptness in the rhizosphere which leads the growth and development of tomato plant.