Effects of fresh mineralized dentin and cementum on socket healing: a preliminary study in dogs

Mahdi Kadkhodazadeh,Majid Ghasemianpour,Negar Soltanian,Gholam Reza Sultanian,Shahriar Ahmadpour,Reza Amid 대한구강악안면외과학회 2015 대한구강악안면외과학회지 Vol.41 No.3

Objectives: Dentin is composed of many minerals and growth factors. Based on this composition, we studied its effect as a possible regenerative material for alveolar healing. Materials and Methods: This study was conducted using four 2.5-year-old mongrel dogs (male; weight, 25 to 30 kg). The third mandibular premolars were carefully mobilized with a dental elevator and then removed using forceps. The crown portions of the extracted teeth were removed with cutters, and the root portions of the remaining teeth were collectively trimmed as closely as possible to 350 to 500 μm. Dentin and cementum (DC) chips harvested from the extracted teeth were soaked in blood and packed into the fresh sockets (autograft). Biopsies were performed at the ends of day 14 and day 56 following implantation. Data were expressed as mean±standard deviation and compared with t-test results. Results: The ratio of S A (bone) to total area of each probe was determined and was 170±16 μm 2 for the control group and 71±14 μm 2 for the DC group, a significant difference (P<0.05). Conclusion: DC particulate grafts offered no improvement in bone regeneration in alveolar extraction sockets.

Receptor activator of nuclear factor kappa-B gene polymorphisms in Iranian periodontitis and peri-implantitis patients

Mahdi Kadkhodazadeh,Zahra Baghani,Ahmad Reza Ebadian,Zahra Kaghazchi,Reza Amid 대한치주과학회 2014 Journal of Periodontal & Implant Science Vol.44 No.3

Purpose: Peri-implantitis and periodontitis are inflammatory and infectious diseases of implantand tooth-supporting tissues. Recently, the role of gene polymorphisms of immune responsecomponents in the relevant pathogenesis has been investigated. The present study wasthe first to evaluate the relationship between two known single nucleotide polymorphisms(SNPs) of the receptor activator of nuclear factor kappa-β (RANK) gene (rs3018362 andrs35211496) in chronic periodontitis and peri-implantitis patients in an Iranian population. Methods: Eighty-one periodontally healthy patients, 38 patients with peri-implantitis, and74 patients with chronic periodontitis were enrolled in this study. DNA was extracted fromblood arm vein samples by using Miller’s salting out technique according to the manufacturer’sinstructions given in the extraction kit. The concentration of DNA samples was measuredusing a spectrophotometer. The genetic polymorphisms of the RANK gene were evaluatedusing a competitive allele specific polymerase chain reaction (KBioscience allele specificPCR) technique. Differences in the frequencies of genotypes and alleles in the diseasedand healthy groups were analyzed using chi-squared statistical tests (P<0.05). Results: Analysis of rs35211496 revealed statistically significant differences in the expressionof the TT, TC, and CC genotypes among the three groups (P=0.00). No statistically significantdifference was detected in this respect between the control group and the chronicperiodontitis group. The expression of the GG, GA, and AA genotypes and allele frequencies(rs3018362) showed no statistically significant difference among the three groups (P=0.21). Conclusions: The results of this study indicate that the CC genotype of the rs35211496RANK gene polymorphism was significantly associated with peri-implantitis and may beconsidered a genetic determinant for peri-implantitis, but this needs to be confirmed byfurther studies in other populations.

Receptor activator of nuclear factor kappa-B gene polymorphisms in Iranian periodontitis and peri-implantitis patients

Kadkhodazadeh, Mahdi,Baghani, Zahra,Ebadian, Ahmad Reza,Kaghazchi, Zahra,Amid, Reza Korean Academy of Periodontology 2014 Journal of Periodontal & Implant Science Vol.44 No.3

Purpose: Peri-implantitis and periodontitis are inflammatory and infectious diseases of implant and tooth-supporting tissues. Recently, the role of gene polymorphisms of immune response components in the relevant pathogenesis has been investigated. The present study was the first to evaluate the relationship between two known single nucleotide polymorphisms (SNPs) of the receptor activator of nuclear factor kappa-${\beta}$ (RANK) gene (rs3018362 and rs35211496) in chronic periodontitis and peri-implantitis patients in an Iranian population. Methods: Eighty-one periodontally healthy patients, 38 patients with peri-implantitis, and 74 patients with chronic periodontitis were enrolled in this study. DNA was extracted from blood arm vein samples by using Miller's salting out technique according to the manufacturer's instructions given in the extraction kit. The concentration of DNA samples was measured using a spectrophotometer. The genetic polymorphisms of the RANK gene were evaluated using a competitive allele specific polymerase chain reaction (KBioscience allele specific PCR) technique. Differences in the frequencies of genotypes and alleles in the diseased and healthy groups were analyzed using chi-squared statistical tests (P<0.05). Results: Analysis of rs35211496 revealed statistically significant differences in the expression of the TT, TC, and CC genotypes among the three groups (P=0.00). No statistically significant difference was detected in this respect between the control group and the chronic periodontitis group. The expression of the GG, GA, and AA genotypes and allele frequencies (rs3018362) showed no statistically significant difference among the three groups (P=0.21). Conclusions: The results of this study indicate that the CC genotype of the rs35211496 RANK gene polymorphism was significantly associated with peri-implantitis and may be considered a genetic determinant for peri-implantitis, but this needs to be confirmed by further studies in other populations.

Evaluation of alveolar crest bone loss via premolar bitewing radiographs: presentation of a new method

Yaser Safi,Mahdi Kadkhodazadeh,Pooria Safai,Mohammad Esmaeelinejad,Nafiseh Shamloo 대한치주과학회 2014 Journal of Periodontal & Implant Science Vol.44 No.5

Purpose: This study aimed to assess the interdental bone level in premolar bitewing radiographswhile retracting the cheeks. Methods: Seventy-two horizontal bone defects were created on dried mandibles and maxillae. The distance from the bone level to the cement-enamel junction of premolars wasdetected by a modified digital caliper (considered the gold standard). The reliability of allradiographs was assessed by intraclass correlation coefficient (ICC), and the validity wascompared to the gold standard using the analysis of variance test. P-values less than 0.05were considered statistically significant. Results: This study showed that the reliability of radiographs without a cheek simulatorand with 0.16 second exposure time was significantly higher than that of the two othergroups (ICC=0.96 compared to 0.93 and 0.88, respectively). The results from the radiographswithout a cheek simulator and with 0.16 second exposure time were more similar tothe gold standard measures than those of the two other groups, although the differencewas not statistically significant. Conclusions: Retracting the buccal soft tissue plays an important role in increasing the accuracyof radiographs in detecting the interdental alveolar bone level and produces moreaccurate results than increasing the exposure time, although it does not have a significantrole in reliability of results.

Comparison of the effect of hand instruments, an ultrasonic scaler, and an erbium-doped yttrium aluminium garnet laser on root surface roughness of teeth with periodontitis: a profilometer study

Amid, Reza,Kadkhodazadeh, Mahdi,Fekrazad, Reza,Hajizadeh, Farzin,Ghafoori, Arash Korean Academy of Periodontology 2013 Journal of Periodontal & Implant Science Vol.43 No.2

Purpose: The present study aimed to measure root surface roughness in teeth with periodontitis by a profilometer following root planning with ultrasonic and hand instruments with and without erbium-doped yttrium aluminium garnet (Er:YAG) laser irradiation. Methods: Sixty single-rooted maxillary and mandibular teeth, extracted because of periodontal disease, were collected. The crowns and apices of the roots were cut off using a diamond bur and water coolant. The specimens were mounted in an acrylic resin block such that a plain root surface was accessible. After primary evaluation and setting a baseline, the samples were divided into 4 groups. In group 1, the samples were root planned using a manual curette. The group 2 samples were prepared with an ultrasonic scaler. In group 3, after scaling with hand instrumentation, the roots were treated with a Smart 1240D plus Er:YAG laser and in group 4, the roots were prepared with ultrasonic scaler and subsequently treated with an Er:YAG laser. Root surface roughness was then measured by a profilometer (MahrSurf M300+RD18C system) under controlled laboratory conditions at a temperature of $25^{\circ}C$ and 41% humidity. The data were analyzed statistically using analysis of variance and a t-test (P<0.05). Results: Significant differences were detected in terms of surface roughness and surface distortion before and after treatment. The average reduction of the surface roughness after treatment in groups 1, 2, 3, and 4 was 1.89, 1.88, 1.40, and 1.52, respectively. These findings revealed no significant differences among the four groups. Conclusions: An Er:YAG laser as an adjunct to traditional scaling and root planning reduces root surface roughness. However, the surface ultrastructure is more irregular than when using conventional methods.

Comparison of the effect of hand instruments, an ultrasonic scaler, and an erbium-doped yttrium aluminium garnet laser on root surface roughness of teeth with periodontitis: a profilometer study

Reza Amid,Mahdi Kadkhodazadeh,Reza Fekrazad,Farzin Hajizadeh,Arash Ghafoori 대한치주과학회 2013 Journal of Periodontal & Implant Science Vol.43 No.2

Purpose: The present study aimed to measure root surface roughness in teeth with periodontitis by a profilometer following root planning with ultrasonic and hand instruments with and without erbium-doped yttrium aluminium garnet (Er:YAG) laser irradiation. Methods: Sixty single-rooted maxillary and mandibular teeth, extracted because of periodontal disease, were collected. The crowns and apices of the roots were cut off using a diamond bur and water coolant. The specimens were mounted in an acrylic resin block such that a plain root surface was accessible. After primary evaluation and setting a baseline, the samples were divided into 4 groups. In group 1, the samples were root planned using a manual curette. The group 2 samples were prepared with an ultrasonic scaler. In group 3, after scaling with hand instrumentation, the roots were treated with a Smart 1240D plus Er:YAG laser and in group 4, the roots were prepared with ultrasonic scaler and subsequently treated with an Er:YAG laser. Root surface roughness was then measured by a profilometer (MahrSurf M300+RD18C system) under controlled laboratory conditions at a temperature of 25°C and 41% humidity. The data were analyzed statistically using analysis of variance and a t-test (P<0.05). Results: Significant differences were detected in terms of surface roughness and surface distortion before and after treatment. The average reduction of the surface roughness after treatment in groups 1, 2, 3, and 4 was 1.89, 1.88, 1.40, and 1.52, respectively. These findings revealed no significant differences among the four groups. Conclusions: An Er:YAG laser as an adjunct to traditional scaling and root planning reduces root surface roughness. However,the surface ultrastructure is more irregular than when using conventional methods.

Evaluation of alveolar crest bone loss via premolar bitewing radiographs: presentation of a new method

Safi, Yaser,Kadkhodazadeh, Mahdi,Safai, Pooria,Esmaeelinejad, Mohammad,Shamloo, Nafiseh Korean Academy of Periodontology 2014 Journal of Periodontal & Implant Science Vol.44 No.5

Purpose: This study aimed to assess the interdental bone level in premolar bitewing radiographs while retracting the cheeks. Methods: Seventy-two horizontal bone defects were created on dried mandibles and maxillae. The distance from the bone level to the cement-enamel junction of premolars was detected by a modified digital caliper (considered the gold standard). The reliability of all radiographs was assessed by intraclass correlation coefficient (ICC), and the validity was compared to the gold standard using the analysis of variance test. P-values less than 0.05 were considered statistically significant. Results: This study showed that the reliability of radiographs without a cheek simulator and with 0.16 second exposure time was significantly higher than that of the two other groups (ICC=0.96 compared to 0.93 and 0.88, respectively). The results from the radiographs without a cheek simulator and with 0.16 second exposure time were more similar to the gold standard measures than those of the two other groups, although the difference was not statistically significant. Conclusions: Retracting the buccal soft tissue plays an important role in increasing the accuracy of radiographs in detecting the interdental alveolar bone level and produces more accurate results than increasing the exposure time, although it does not have a significant role in reliability of results.

Sinus floor elevation and simultaneous implant placement in fresh extraction sockets: a systematic review of clinical data

Mehdi Ekhlasmandkermani,Reza Amid,Mahdi Kadkhodazadeh,Farhad Hajizadeh,Pooria Fallah Abed,Lida Kheiri,Aida Kheiri 대한구강악안면외과학회 2021 대한구강악안면외과학회지 Vol.47 No.6

Combining different procedures to reduce the number of surgical sessions and patient discomfort in implant placement and sinus floor elevation has been recommended, and evidence supports good outcomes. The aim of this study was to review the results of clinical studies on sinus floor elevation through extraction sockets and simultaneous immediate posterior implant placement. An electronic search was carried out in PubMed, Scopus, and Web of Science to find English articles published in or before August 2020. A manual search was also performed. Titles, abstracts, and the full-text of the retrieved articles were studied. Thirteen studies met our eligibility criteria: 6 retrospective case series, 3 case reports, 2 prospective cohort case-series, 1 prospective case series, and 1 randomized controlled trial. Overall, 306 implants were placed; 2 studies reported implant survival rates of 91.7% and 98.57%. The others either did not report the survival rate or reported 100% survival. Sinus floor elevation through a fresh extraction socket and simultaneous immediate implant placement appears to be a predictable modality with a high success rate. However, proper case selection and the expertise of the clinician play fundamental roles in the success of such complex procedures.

A comparison of magnetostrictive and piezoelectric ultrasonic scaling devices: an in vitro study

Hojatollah Yousefimanesh,Maryam Robati,Mahdi Kadkhodazadeh,Reza Molla 대한치주과학회 2012 Journal of Periodontal & Implant Science Vol.42 No.6

Purpose: The effects of magnetostrictive and piezoelectric devices on tooth surfaces seem to differ with regard to the root surface roughness they produce. This study aimed to compare the results of scaling using magnetostrictive and piezoelectric devices on extracted teeth. Methods: Forty-four human extracted teeth were assigned to four study groups (n=11). In two groups (C100 and C200), the teeth were scaled using a magnetostrictive device and two different lateral forces: 100 g and 200 g, respectively. In the other two groups (P100 and P200), the teeth were scaled with a piezoelectric device with 100 g and 200 g of lateral force, respectively. The teeth were scaled and the data on the duration of scaling and the amount of surface were collected and analyzed using the t-test. Results: The mean time needed for instrumentation for the piezoelectric and magnetostrictive devices was 50:54 and 41:10,respectively, but their difference was not statistically significant (P=0.171). For root surface roughness, we only found a statistically significantly poorer result for the C200 group in comparison to the P200 group (P=0.033). Conclusions: This study revealed that applying a piezoelectric scaler with 200 g of lateral force leaves smoother surfaces than a magnetostrictive device with the same lateral force.

A comparison of magnetostrictive and piezoelectric ultrasonic scaling devices: an in vitro study

Yousefimanesh, Hojatollah,Robati, Maryam,Kadkhodazadeh, Mahdi,Molla, Reza Korean Academy of Periodontology 2012 Journal of Periodontal & Implant Science Vol.42 No.6

Purpose: The effects of magnetostrictive and piezoelectric devices on tooth surfaces seem to differ with regard to the root surface roughness they produce. This study aimed to compare the results of scaling using magnetostrictive and piezoelectric devices on extracted teeth. Methods: Forty-four human extracted teeth were assigned to four study groups (n=11). In two groups (C100 and C200), the teeth were scaled using a magnetostrictive device and two different lateral forces: 100 g and 200 g, respectively. In the other two groups (P100 and P200), the teeth were scaled with a piezoelectric device with 100 g and 200 g of lateral force, respectively. The teeth were scaled and the data on the duration of scaling and the amount of surface were collected and analyzed using the t-test. Results: The mean time needed for instrumentation for the piezoelectric and magnetostrictive devices was 50:54 and 41:10, respectively, but their difference was not statistically significant (P=0.171). For root surface roughness, we only found a statistically significantly poorer result for the C200 group in comparison to the P200 group (P=0.033). Conclusions: This study revealed that applying a piezoelectric scaler with 200 g of lateral force leaves smoother surfaces than a magnetostrictive device with the same lateral force.