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      • KCI등재

        Weld bead characterization of flat wire electrode in gmaw process part II: a numerical study

        K. Sripriyan,M. Ramu,P. R. Thyla,K. Anantharuban 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.6

        A comprehensive numerical model was developed to predict the transient temperature distribution during the gas metal arc welding process with the use of flat wire electrode (FW-GMAW). Since the perimeter of the flat wire electrode has increased, the turbulence of the molten characteristics is altered due to the effect of electromagnetic force. To validate, a general double ellipsoid moving heat source model was adopted and the numerical analysis predicted that the peak temperature had risen due to increased heat power density in the flat wire electrode. The proposed approach of numerical analysis confirmed that, the initial arc and steady state temperature was high for FW-GMAW process. In addition, the consequence of temperature distribution in FW-GMAW was analyzed and its significance to weld bead geometry was broadly discussed in this paper.

      • KCI등재

        Design, analysis and fabrication of polyamide/ hydroxyapatite porous structured scaffold using selective laser sintering method for bio-medical applications

        T. Kumaresan,R. Gandhinathan,M. Ramu,M. Ananthasubramanian,K. Banu Pradheepa 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.11

        Both medical and engineering sciences play a vital role in human health diagnose and treatment. In this scientific world, the technology of alternative bone scaffold is one of the remedial sources for bone loss treatment. This research paper focuses on the design and analysis of porous scaffold made of additive manufacturing process. Various types of biocompatible materials with different characteristics are used for this bone scaffold. In order to have high load bearing capacity, a material composition of biocompatible Polyamide (PA) combined with Hydroxyapatite (HA) is used in this research work. HA has the character of greater cell seeding growth of tissue cells and hence added in the composition. The porous scaffold models with different configuration of cubical pore, spherical pore, shifted cubical pore, shifted spherical pore are designed using Solidworks software with a pore size of 800 microns and the porosity ranging from 40 % to 70 %. All the CAD models are analyzed using ANSYS software. Based on the static structural analysis, the shifted cubical scaffold posses lesser stress concentration, and thus selected for fabrication using Selective Laser Sintering machine. The specimens are fabricated in three different build orientations and the load bearing strength is found out using experimentation. The different material composition of 100 %PA, 95 %PA: 5 %HA, 90 %PA: 10 %HA and 85 %PA: 15 %HA are considered for this study.

      • KCI등재

        Characterization of ZrC reinforced AA6061 alloy composites produced using stir casting process

        T. Satish Kumar,S. Shalini,M. Ramu,Titus Thankachan 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.1

        In the present study, effect of ZrC vol.% on mechanical properties of AA6061 metal matrix composites (MMCs) produced via stir casting technique was investigated. The vol.% of ZrC particles was varied as 5,10 and 15. The composites were characterized for its microstructure and mechanical properties and their relationships were obtained. The scanning electron microscope (SEM) images revealed uniform distribution and good bonding between the AA6061 alloy and the ZrC particles. The mechanical properties of the AA6061 alloy was found to significantly improve with the addition of ZrC particles from 5 to 15 vol.%, the hardness increased from 32 to 68 HV, yield strength increased from 50 to 86 MPa and the ultimate tensile strength increased from 118 to 165 MPa. However, the % of elongation of the composite samples decreased with 15 vol.% addition of ZrC particles. Sliding wear behaviour of the composites was investigated using a pin-on-disc wear tester at a load of 9.8 N and addition of ZrC particles was significantly found to reduce the wear rate of AA6061 alloy.

      • KCI등재

        Overexpression of AtNAC2 (ANAC092) in groundnut (Arachis hypogaea L.) improves abiotic stress tolerance

        Mahesh Patil,T. G. Prasad,S. V. Ramu,P. Jathish,Rohini Sreevathsa,P. Chandrashekar Reddy,M. Udayakumar 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.2

        Groundnut (Arachis hypogaea L.) is animportant oilseed crop grown in semi-arid tropics where itexperiences moisture stress at different stages of growthresulting in reduced growth and productivity. In this study,we report that the stress tolerance of groundnut can beimproved by overexpression of stress-specific transcriptionfactor through transgenic approach. In silico electronicnorthernanalysis of AtNAC2 showed increased expressionunder different abiotic stresses. The transcript levels of ahomolog of AtNAC2 gene were upregulated under differentdrought regimes in groundnut. Groundnut transgenicsoverexpressing AtNAC2 showed enhanced tolerance todrought and salinity with improved yield under waterlimitedconditions. The study demonstrates that AtNAC2 isa potential candidate gene to improve stress tolerance bytransgenic approach.

      • KCI등재

        Transcription factors controlling the expression of oxidative stress associated genes in rice (Oryza sativa L.)

        Sujitha D.,Kumar H. G. Jalendra,Thapliayal Garima,Pal Garima,Vanitha P. A.,Uttarkar Akshay,Patil Mahesh,Reddy B. H. Rajashekar,Niranjan Vidya,Rayalcheruvu Usha,Govind Geetha,Udayakumar M.,Vemanna Ramu 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.6

        Reactive oxygen species (ROS) increases under stress and damages cellular processes leading to decrease in productivity. Many genes have been known to be involved in scavenging ROS. We report the identification of master regulators of oxidative stress responsive genes from contrasting rice genotypes. Using microarray analysis, we identified 52 differentially expressed transcription factors (TFs) from the contrasting rice genotypes under oxidative stress. Upregulation of these TFs induces the expression of many genes in resistant or sensitive genotypes. The promoters of these TFs are enriched with reactive oxygen species binding elements (ROSE). The promoter analysis of genes that respond to oxidative stress also revealed that these TF binding sites were present and that these genes expressed differently in contrasting rice genotypes. The transcript levels of TFs correlate with expression level of stress responsive genes coding for various pathways such as polyol, ABA, JA biosynthesis and signaling. Functional validation of HSF-C1a using virus-induced gene silencing (VIGS), showed reduced expression of its target genes. Our study demonstrates that identified TFs could act as major transcriptional regulators of oxidative stress tolerance. These TFs can be used as markers and are potential candidates to improve stress tolerance in plants.

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