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Han, M.S.,Wang, P.,Kim, J.H.,Cho, S.Y.,Park, B.S.,Kim, J.H.,Katano, T.,Kim, B.H. G. Fischer 2016 PROTIST -JENA- Vol.167 No.1
Prorocentrum micans is an extremely variable dinoflagellate species, with many different local forms reported worldwide. Because of this morphological diversity, it is important to establish whether these various forms belong to P. micans sensu stricto. For this study, P. micans-like specimens were isolated from several localities in the southern coastal waters of Korea and Japan. The morphological characteristics and the molecular signatures of P. micans were re-examined. Moreover, a new Prorocentrum species, Prorocentrum koreanum sp. nov. was established through detailed light microscopy and scanning electron microscopy observations. Examination of the periflagellar platelets revealed that P. koreanum sp. nov. differs from P. micans. Furthermore, P. koreanum and P. micans exhibited different distribution patterns of trichocyst pores. Through molecular phylogeny analysis of small subunit (SSU) rRNA, internal transcribed spacer (ITS), and large subunit (LSU) rRNA sequence, we found P. koreanum to be more closely related to P. mexicanum and P. rhathymum than to P. micans. Additionally, ITS2 compensatory base changes also provide strong evidence to support P. koreanum and P. micans being separate species.
Jung, S.W.,Kim, B.-H.,Katano, T.,Kong, D.-S.,Han, M.-S. Blackwell Publishing Ltd 2008 Journal of applied microbiology Vol.105 No.1
<P>Abstract</P><P>Aims: </P><P>The study of an algicidal activity and mechanism of the isolated <I>Pseudomonas fluorescens</I> HYK0210-SK09 (SK09) against a winter bloomed harmful diatom, <I>Stephanodiscus hantzschii.</I></P><P>Methods and Results: </P><P>SK09 was isolated from the Paldang reservoir, Korea and used to biological control of <I>S. hantzschii</I>. The inoculation of SK09 at the final density of 5 × 10<SUP>6</SUP> cells ml<SUP>−1</SUP> caused degradation of >90% of <I>S. hantzschii</I> cells within 5 days. The algal cell lysis was achieved by a direct attack of the bacteria to the diatom cells, and the algicidal compound was located in the cytoplasm of the cell. As SK09 did not suppress <I>Microcystis aeruginosa</I>, <I>Anabaena cylindrica</I>, <I>Coelastrum astroideum</I> or <I>Cyclotella meneghiniana</I>, it appeared to attack <I>S. hantzschii</I> in a species-specific manner. Testing in an indoor mesocosms confirmed that SK09 effectively reduced <I>S. hantzschii</I> cells by 88% within 9 days.</P><P>Conclusions: </P><P>This bacterium is useful in regulating blooms of <I>S. hantzschii</I>. However, it should be studied in the future that their impact in shaping phytoplankton community and their activity in natural environments.</P><P>Significance and Impact of the Study: </P><P>The bacterium enabled us to develop a new strategy, to understand the interaction for anthropogenic control of harmful algal blooms in nature.</P>
Yuusuke YAMAGUCHI,A. Fukuyama,H. Nakajima,H. Higaki,K. Nakagome,K. Nemoto,M. Katano,M. Ichimura,S. Kakimoto,T. Cho 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.III
A wide-band radio-frequency (RF) probe system was newly constructed for the active diagnostic of the eigenmode formations in the ion-cyclotron range of frequencies in the GAMMA 10 tandem mirror. A bar-type antenna was installed in the peripheral region in the minimum-B anchor cell. A low-power RF pulse with the frequency sweep from 8 to 20 MHz is applied to the antenna. The waves excited in the plasma are detected with a magnetic probe. The excitation of eigenmodes is described by using the antenna-plasma-probe transfer function. The transfer function can be obtained from the antenna current signal and the magnetic probe signal. When the real and the imaginary part of the transfer function are plotted in the complex plane, the resultant curves are approximately circles, indicating an eigenmode formation. It is found that the several fast wave eigenmodes can be excited in the present experimental condition.