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        Invasive Success of Small Hive Beetles in Honey Bee Colonies with Screen and Solid Bottom Boards

        Lilia I. de Guzman,Amanda M. Frake 한국양봉학회 2019 韓國養蜂學會誌 Vol.34 No.4

        Small hive beetles (SHBs) are invasive pests of Apis mellifera colonies. Within the last five years, SHBs have been detected in Asia, Europe and South America. The high colonization capability of SHB is facilitated by their small size, agility, long survival and ability to conduct long-range dispersal flights. We compared the invasion success of adult SHBs in colonies with screen (8-mesh) or solid bottom boards facing inward or outward of the apiary. Notched beetles were released at the center of an apiary that was devoid of honey bee colonies for about two months and tracked their presence in the colonies for four months. Overall, colonies with screen and solid bottom boards had similar numbers of beetles (~4 notched and 4 no notch). No influence of hive entrance orientation on the number of beetles was also observed. This suggests that the use of screen bottom boards does not promote an increase of SHB population in honey bee colonies since beetles invaded colonies through hive entrances and not through the screen mesh. Thus, screen bottom boards can be used as a component of an Integrated Pest Management program for Varroa mite control.

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        Responses of Varroa-resistant honey bees (Apis mellifera L.) to Deformed wing virus

        Kitiphong Khongphinitbunjong,Lilia I. de Guzman,Thomas E. Rinderer,Matthew R. Tarver,Amanda M. Frake,Yanping Chen,Panuwan Chantawannakul 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4

        The negative impact of Deformedwing virus (DWV) on European honey bees Apis mellifera is magnified by Varroa destructor parasitism. This study compared the responses of two Varroa-resistant honey bee stocks, pure Russian honey bees (RHB) and out-crossed Varroa Sensitive Hygienic bees, Pol-line (POL) to DWV infection to that of Varroa- susceptible stock, Italian honey bees (IHB). Two-day-old larvae were fed with DWV lysate in different concentrations: undiluted DWV lysate (D1), D1:100, and D1:1000. The unfed larvae served as negative control. Combs containing test larvae were exposed to a common environment during their development using host colonies. Our results showed that only POL displayed variation in DWV levels when fed different DWV concentrations. POL fed highest concentration of DWV inoculum had the highest increase in DWV level than those fed low concentrations and unfed POL. This high increase in DWV level probably contributed to the decrease in the survival and median longevity (LT50) of D1-fed POL. Weights of newly eclosed D1-fed POL were similar to those of the two controls and DWV-fed bees. However, within IHB, D1-fed bees showed significant reductions in weight, days of survival and LT50. Regardless of the concentrations of DWV inoculum, the DWV levels were similarly low within RHB; adult bees had similar weights. Overall, larvae fed D1 had the highest rate of wing deformation. POL and RHB had numerically lower proportions of bees with deformed wings. This study suggests that RHB showed some degree of resistant to DWV as shown by no reduction on weight and numerically lower proportion of wing deformity when compared with the other bee stocks.

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