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Yao Zhiming,Fan Jingyan,Dai Jun,Yu Chen,Zeng Han,Li Qingzhi,Hu Wei,Yan Chaoyue,Hao Meilin,Li Haotian,Li Shuo,Liu Jie,Huang Qi,Li Lu,Zhou Rui 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.6
Tylosin is a potent veterinary macrolide antibiotic produced by the fermentation of Streptomyces fradiae; however, it is necessary to modify S. fradiae strains to improve tylosin production. In this study, we established a high-throughput, 24-well plate screening method for identifying S. fradiae strains that produce increased yields of tylosin. Additionally, we constructed mutant libraries of S. fradiae via ultraviolet (UV) irradiation and/or sodium nitrite mutagenesis. A primary screening of the libraries in 24-well plates and UV spectrophotometry identified S. fradiae mutants producing increased yields of tylosin. Mutants with tylosin yield 10% higher than the wild-type strain were inoculated into shake flasks, and the tylosin concentrations produced were determined by highperformance liquid chromatography (HPLC). Joint (UV irradiation and sodium nitrite) mutagenesis resulted in higher yields of mutants with enhanced tylosin production. Finally, 10 mutants showing higher tylosin yield were re-screened in shake flasks. The yield of tylosin A by strains UN-C183 (6767.64 ± 82.43 μg/ml) and UN-C137 (6889.72 ± 70.25 μg/ml) was significantly higher than that of the wild-type strain (6617.99 ± 22.67 μg/ml). These mutant strains will form the basis for further strain breeding in tylosin production.
Zhang Qingzhi,Lu Haifeng,Li Yongdong,Chai Jianyun 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5
Free Piston Linear Generator (FPLG) is an ideal power resource in Hybrid Electric Vehicle (HEV) for its potential advantages of low friction loss, variable compression ratio, being suitable for various fuels, high thermal efficiency, etc. No FPLG has been successfully applied to an actual vehicle as lack of reliable control. The first part of this paper consists of introduction to FPLG and its research status. Structure and working principle of FPLG are analyzed in the second part. Then the thermodynamic process is analyzed and mathematical models of all kinds of FPLG using real-time motion control strategy based on location domain are established. After these, verifying work of the six different models established before and the realtime control strategy is carried out. Results show that the mathematical models of FPLG based on location domain work well and the control strategy effects with proper linear generator.
Selective surface functionalization at regions of high local curvature in graphene
Wu, Qingzhi,Wu, Yaping,Hao, Yufeng,Geng, Jianxin,Charlton, Matthew,Chen, Shanshan,Ren, Yujie,Ji, Hengxing,Li, Huifeng,Boukhvalov, Danil W.,Piner, Richard D.,Bielawski, Christopher W.,Ruoff, Rodney S. The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.7
<P>Monolayer graphene was deposited on a Si wafer substrate decorated with SiO<SUB>2</SUB> nanoparticles (NPs) and then exposed to aryl radicals that were generated <I>in situ</I> from their diazonium precursors. Using micro-Raman mapping, the aryl radicals were found to selectively react with the regions of graphene that covered the NPs. The enhanced chemical reactivity was attributed to the increased strain energy induced by the local mechanical deformation of the graphene.</P> <P>Graphic Abstract</P><P>Due to the increased strain energy caused by local mechanical deformation, aryl radicals were found to selectively react with regions of graphene that were curved by underlying SiO<SUB>2</SUB> nanoparticles. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2cc36747e'> </P>
Qi Li,Qingzhi Wang,Mingjun Qi,Jianlong Ge,Rihao Cong 한국유전학회 2011 Genes & Genomics Vol.33 No.3
A total of 113 novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the Pacific oyster (Crassotrea gigas). Polymorphisms of these markers were evaluated in a wild population of 30 individuals. The number of alleles per locus ranged from 2 to 27 with an average of 6.3, and the observed and expected heterozygosities varied from 0 to 0.9667 and from 0.0333 to 0.9701, respectively. Mendelian segregations were tested for 24 of the markers that were polymorphic in one family produced by single-pair mating. Null alleles were discovered at four loci. Nine tests of segregation ratios revealed significant departures from expected Mendelian ratios. As a useful addition to the collection of the microsatellites that are now available for C. gigas, these EST-SSR markers will help the advance in investigation of QTL mapping and genetic diversity in this species.
AN IMPROVED METHOD FOR TRANSFERRING GRAPHENE GROWN BY CHEMICAL VAPOR DEPOSITION
YUJIE REN,CHAOFU ZHU,WEIWEI CAI,HUIFENG LI,YUFENG HAO,YAPING WU,SHANSHAN CHEN,QINGZHI WU,RICHARD D. PINER,RODNEY S. RUOFF 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.1
In this paper, we report an improved transfer of graphene by directly picking up the graphene with target substrates and the comparison of such transferred graphene samples with other graphene samples transferred by the commonly used "PMMA-based transfer" method. Raman spectroscopy studies show that this "direct transfer method" does not degrade the graphene structure and field effect transistor measurements show that it does not introduce any extra doping in graphene; in contrast, PMMA-based transfer samples have strong n-type doping. We also find that graphene samples achieved by this direct transfer method show higher mobility than graphene obtained from the PMMA-based transfer method.