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RISS 인기검색어
- 검색키워드
- 저자 : Lesley L . Rhodes (검색결과 6 건)
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Comparative lectin binding patterns of Cochlodinium polykrikoides Margalef
Rhodes, Lesley L.,Cho, Yong-Chul,Cho, Eun-Seob The Korean Society of Oceanography 2000 Journal of the Korean Society of Oceanography Vol.35 No.3
Four different FITC-conjugated lectins were used to visually evaluate lectin binding activity by optical staining quality using confocal laser scanning microscopy (CLSM) of Cochzodinium polykrikoides in nature (wild type) and culture (cultured type). Cells from the field and cultures treated with ConA fluoresced only at the outer cell wall, and the abundance and distribution of the fluorescent signal were similar. Treatment with PWM and HPA did not elicit fluorescence at the cell surface, but the wild type exposed to HPA showed greater binding than did the cultured cells, possibly due to greater concentrations of glucosamine. The wild type cells treated with LBL lectin showed a strong green fluorescence on the cell surface, whereas cultured cells did not. Signal intensity and abundance were greater than for any other lectins tested in this study. These results suggest that wild type and cultured type are significantly different based on surface sugar production. In particular, the wild type cells apear richer in galactosamine-like moieties. Neither glucose nor mannose-like moieties were present in either wild types or cultured cells.
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Cho Eun Seob,Rhodes Lesley L.,Kim Hak Gyoon The Korean Society of Fisheries and Aquatic Scienc 1999 Fisheries and Aquatic Sciences Vol.2 No.1
The blooms caused by Fibrocapsa japonica have occurred regularly in New Zea1andean coastal waters, and no fish kills and economic impacts have been reported. However, Fibrocapsa japonica in Japan killed caged juvenile fish and has been regarded as a harmful micro algae. In this comparative study a New Zealand isolate was found to be morphologically similar to a Japanese isolate, although slightly larger on average than. Optimal temperatures for growth differed, with fastest growth rates occurring at $22-24^{\circ}C$ for the New Zealand strain and $16-22^{\circ}C$ for the Japanese strain, with a decrease in growth rate exhibited by the latter at $25^{\circ}C$. Both isolates had low salinity optima of 20-25 PSU, although they grew between 15 and 30 PSU. Growth declined significantly for both strains above 30 PSU.
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KIM, Hak Gyoon,Cho, Eun Seob,Lesley L . Rhodes 한국수산학회 1999 Fisheries and Aquatic Sciences Vol.2 No.1
The blooms caused by Fibrocapsa japonica have occurred regularly in New Zealandean coastal waters, and no fish kills and economic impacts have been reported. However, Fibrocapsa japonica in Japan killed caged juvenile fish and has been regarded as a harmful microalgae. In this comparative study a New Zealand isolate was found to be morphologically similar to a Japanese isolate, although slightly larger on average than. Optimal temperatures for growth differed, with fastest growth rates occurring at 22∼24℃ for the New Zealand strain and 16∼22℃ for the Japanese strain, with a decrease in growth rate exhibited by the latter at 24℃. Both isolates had low salinity optima of 20∼25 PSU, although they grew between 15 and 30 PSU. Growth declined significantly for both strains above 30 PSU.
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The Comparison of Two Strains of Fibrocapsa japonica(Raphidophyceae)in New Zealand and Japan
KIM, Hak Gyoon,Cho, Eun Seob,Lesley L . Rhodes 한국수산학회 1999 Fisheries and Aquatic Sciences Vol.2 No.1
Fibrocapsa japonica (Raphidophyceae) is regarded as a harmful algal bloom organism in Japanese waters, where it has been linked to fish kills. Fibrocapsa is a common species in New Zealand coastal waters, particularly in the Hauraki Gulf, where it has regularly bloomed in the spring under El Nin~o climate conditions for the past six years. The New Zealand isolate had 1.4 times more total polyunsaturated acids than the Japanese isolate under the same growth conditions, suggesting that eicosapentaenoic acid in particular could be used as a discriminating chemotaxonomic marker. The molecular probes tested showed no differential binding of the raphidophytes to lectins, but oligonucleotide probes targeted F. japonica ribosomal RNA bound specifically to both isolates. Neither strain was toxic in mouse or neuroblastoma bioassays. There is no evidence that the New Zealand F japonica isolates investigated to date produce ichthyotoxins.
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Cho, Eun-Seob,Park, Jong-Gyu,Kim, Hak-Gyoon,Kim, Chang-Hoon,Rhodes, Lesley L.,Chung, Chang-Soo The Korean Society of Oceanography 1999 Journal of the Korean Society of Oceanography Vol.34 No.3
Since toxic Alexandrium catenella and non-toxic A. fraterculus are morphologically similar, they are difficult to discriminate under the light microscope. However, a novel technology, such as fluorescein isothiocyanate (FITC)-conjugated lectin probes enables easy and rapid differentiation. Toxic A. catenella bound seven different lectins, whereas the non-toxic A. fratercuzus did not bind Arachis hypogaea (PNA) lectin. In addition, Pseudo-nitrschia species in this study were also difficult to identify to species level with light microscope techniques, but it was possible to classify them using fluorescent lectins. Pseudo-nitzschia multistriata, P. subfraudulenta and P. pungens bound Canavalia ensiformis (ConA), whereas P. subpaclfica did not, and P. pungens also bound Ricinus communis (RCA). These results imply that lectin could be used as a critical tool in the differentiation of P. multistriata, P. subfraudulenta and P. pungens. However, P. subpacifica was not differentiated by the lectins tested. Therefore, it isconcluded that lectin probes are useful for discriminating toxic A. catenella from non-toxic A. fraterculus, and for the identification of some Pseudo-nitzschia species. In addition, this method has a great potential to speed and detection between non-toxic and toxic harmful algal blooms (HABs) in Korean biotoxin monitoring systems.
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LEE, SANG JUN,KIM, Hak Gyoon,LEE, Sam Geun,Hong, Yong Ki,Cho, Eun Seob,Seo, Gwi Moon,Lesley L . Rhodes 한국수산학회 1998 Fisheries and Aquatic Sciences Vol.1 No.2
Harmful microalgae isolated from Korean coastal waters, were tested with FITC-conjugated lectins and observed by epifluorescent microscopy to distinguish each other. Strain-specific sugar composition at the cell surface was suggested by the affinity of lectins to different microalgae. The microalgae Cochlodinium polykrikoides (CP-1) and Gymnodinium A₃(GA₃-1), are morphologically similar, but exhibited different binding activity with the lectins ECA, HPA and WGA. In Peridiniales, the microalga Alexandrium tamarense (AT) bound HPA and WGA, but Scrippsiella trochoidea (ST-1) did not bind those lectins. Three species of Prorocentrum also exhibited different binding specificity with HPA, PHA and SBA. A nontoxic Korean isolate of Heterosigma akashiwo (HA-2) bound ConA, PEA and UFA. These results suggest that lectins are useful in discriminating morphologically similar species, as well as different species or strains within the same genus.
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