피부 병변을 동반한 악성조직구증 1예

조은택,박용관,김진호,강정원,천영욱,전익섭,박유환,전춘해 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

Malignant histiocytosis, originally described in 1939 as histiocytic medullary reticulosis by Scott and Robb-Smith, is a rare histiocytic proliferative disorder that often shows an acute onset and used to progress to death within a few months. This disorder characterized clinically by fever, generalized weakness, lymphadenopathy and hepatosplenomegaly, and shows a variable range of pancytopenia. Extranodal involvement is common, with an incidence ranging from 50% to as high as 90%, skin involves8ment was noted in 10% to 15%. Typical skin lesions were mainly founded in extremity. i.e. erythematous papule and nodule occasionally become to necrosis and ulceration. We experienced one case of malignant histiocytosis in a 46-years-old female. The major clinical findings are fever, malaise, hepatosplenomegaly and erythematous skin lesion. In the laboratory study, pancytopenia is noted on the peripheral blood. And also aggregation of many atypical histiocytes were shown on skin and bone marrow biopsy. So we reported one related case with malignant histiocytosis as well as reviewing literature .

골분을 이용한 구리 흡착 첨착

김은정,박정훈,김정섭,유현철,이봉섭,곽명화,우성훈,박승조 東亞大學校 環境問題硏究所 2005 硏究報告 Vol.27 No.1

Spent pig bone was consist of hydroxyapatite. And then we thought that it have a possibility as a adsorbent. The preparation of bone char prepared from spent pig bone in this study. We study on the recycling possibility as a adsorbent is able to adsorb copper ion from their single aqueous solutions. The results are summarized as follows. Bone char that prepared from spent pig bone at 105, 400, 700℃. The adsorption removal efficiency of copper ion onto bone char as adsorbent was above 90% for 15 hours. Increase in the initial pH of the copper ion solution result in an increase in the copper ion uptake per unit weight of the adsorbent. Freundlich isotherm model was found to be applicable for the experiment data of copper ion. The results showed that spent pig bone can be used for the absorption of the copper ion.

2004년 7월 봇돌바다의 썰물, 전류 및 밀물시 수온과 염분 분포

최용규,조은섭,이용화,이영식 한국환경과학회 2005 한국환경과학회지 Vol.14 No.2

Based on the observation on 20, 23 and 26 July 2004, the distributions of temperature, salinity and stratification was investigated in relation to ebb, turn of tide and flood. The results are as follows: 1) The high temperature and low saline water with 23.5-24.0℃ and 32.4~33.0psu existed at Naro Island. 2) The cold surface water below 21.00 and 33.0~33.4psu appeared in the area near Gae Island and Geumo Island. 3) The cold and saline water, below 24.0℃ at the surface and 17.0℃ near the bottom, 32.8~33.8psu at the surface and 33.8~34.0psu near the bottom, existed in Sori Island. These waters were more saline compared to the South Sea Coastal Water with about 31.8psu. This suggests that the oceanic saline water intruded into the Bottol Bada through the area near Sori Island. The stratification appeared during all the observation periods due to a high solar radiation of 22MJ/m², and a weak wind speed of 2.9m/s on the average while the mean speed of wind in July is around 3.9 m/s. It qualitatively suggested that the stratification was maintained during the observation periods because of a high solar radiation, a weak wind speed and intrusion of saline oceanic water.

2004년 7월 봇돌바다의 영양염과 Chlorophyll-a의 단기 변동

최용규,조은섭,권기영,이용화,이영식 한국환경과학회 2005 한국환경과학회지 Vol.14 No.4

In order to study the temporal variations of nutrients and chlorophyll-a in the Bottol Bada, three field observations were carried out on 20, 23 and 26 July, 2004. The low N:P values exhibit nitrogen deficiency during the periods of observation. This result is not representative of typical summer environment in the southern coast of Korea. The possible mechanisms are as follows: 1) The freshwater inflow was not sufficient for the supply of nitrogen because the total precipitation was 11.9 mm in July, 2004. This amount is no more than 5% in normal precipitation in July. 2) There was an inflow of oceanic water under the subsurface into the Bottol Bada. Even though the oceanic water comprises more nutrients, it produces the stratification between the surface and the subsurface water and seems to prevent the supply of nutrinets to the surface layer. 3) The high chlorophyll-a concentration of 1.2 ㎍/L was shown near the narrow channel between Gae-do and Geumo-do. This seems to be resulted from the inflow of water from Gamak Bay.

The Rapid Differentiation of Toxic Alexandrium and Pseudo-nitzschia Species Using Fluorescent Lectin Probes

Cho, Eun-Seob,Park, Jong-Gyu,Kim, Hak-Gyoon,Kim, Chang-Hoon,Rhodes, Lesley L.,Chung, Chang-Soo The Korean Society of Oceanography 1999 Journal of the Korean Society of Oceanography Vol.34 No.3

Since toxic Alexandrium catenella and non-toxic A. fraterculus are morphologically similar, they are difficult to discriminate under the light microscope. However, a novel technology, such as fluorescein isothiocyanate (FITC)-conjugated lectin probes enables easy and rapid differentiation. Toxic A. catenella bound seven different lectins, whereas the non-toxic A. fratercuzus did not bind Arachis hypogaea (PNA) lectin. In addition, Pseudo-nitrschia species in this study were also difficult to identify to species level with light microscope techniques, but it was possible to classify them using fluorescent lectins. Pseudo-nitzschia multistriata, P. subfraudulenta and P. pungens bound Canavalia ensiformis (ConA), whereas P. subpaclfica did not, and P. pungens also bound Ricinus communis (RCA). These results imply that lectin could be used as a critical tool in the differentiation of P. multistriata, P. subfraudulenta and P. pungens. However, P. subpacifica was not differentiated by the lectins tested. Therefore, it isconcluded that lectin probes are useful for discriminating toxic A. catenella from non-toxic A. fraterculus, and for the identification of some Pseudo-nitzschia species. In addition, this method has a great potential to speed and detection between non-toxic and toxic harmful algal blooms (HABs) in Korean biotoxin monitoring systems.

Effect of environmental impact to molecular expression of heat-shock protein (HSP70) in oyster Crassostrea gigas from Gamak bay, Korea.

Cho, Eun-Seob,Jeong, Hee-Dong The Academy 2012 Journal of environmental biology Vol.33 No.3

<P>The cDNA of Crassostrea gigas HSP70 was cloned and rapid amplification of cDNA (RACE) techniques were used. The full length of HSP70 cDNA was 2045 bp, consisting of a 5' terminal untranslated region (UTR) of 80 bp, a 3' terminal UTR 146 bp, and an open reading frame (ORF) of 1829 bp encoding deduced 620 amino acids. The HSP70 cDNA contained HSP70 family signatures, ATP-GTP binding site motif, tetrapeptide (GGMP) and conserved carboxyl terminal region (EEVD) at C-terminal of deduced amino acid sequence. BLAST analysis revealed that the HSP70 gene has an extreme similarity of 98.9% with C. gigas (AF144646). Northern blotting was used to examine the expression of HSP70 mRNA in the gill tissue of the oyster obtained from surface, middle and bottom layers. The HSP70 mRNA observed the samples taken from middle and bottom layers in September and February, but samples from the surface layer did not find a signal intensity of HSP70 mRNA transcript. Consequently, it seems that the oyster occurring middle and bottom layer have been stressed during the period of summer and winter, which is associated with the massive mortality in Gamak bay.</P>

Genetic Responses of the Ark Shell Scapharca broughtonii Schrenck to Environmental Shock: High Temperatures and Long Exposure Times

Cho, Eun-Seob,Jung, Choon-Goon,Shin, Yun-Kyung 한국해양학회 2009 Ocean science journal Vol.44 No.2

Genetic responses of non-high temperature exposed and high temperature exposed ark shell (Scapharca broughtonii) cells collected from Dukyang Bay in August 2007 to environmental shock have been compared. In the analysis of two-dimensional electrophoresis, seven protein spots were found. They have no matching protein in the database, but the number of protein (#1) in unstressed ark shells differed from that of stressed ark shells, indicating a strong signal on the gel and had a homology with arginine kinase (molecular weight, 38 kDa; pI 8.3). The predicted tertiary structure of arginine kinase formed finger-like antiparalled b-sheet projections. A small a-helical N-terminal domain is followed by a larger C-terminal domain. Extracted total RNA was reverse transcribed and amplified by PCR with primer used in this study. Mantle, gill and visceral in non-high temperature exposed ark shell showed some levels of arginine kinase transcripts, but not the foot. These cells also showed amplified fragments on the gel exposed to $20^{\circ}C,\;23^{\circ}C$ and $27^{\circ}C$, but the foot cell had no expression of arginine kinase gene at $27^{\circ}C$ for 3 hrs. These findings provide important insights into the possible molecular mechanisms in which no expression of arginine kinase is contributed to preventing the binding of arginine and actin and difficult to move foot muscle.

Genetic Study of the Class Dinophyceae Including Red Tide Microalgae Based on a Partial Sequence of SSU Region : Molecular Position of Korean Isolates of Cochlodinium polykrikoides Margalef and Gyrodinium aureolum Hulburt

Cho, Eun-Seob Korean Society of Life Science 2004 생명과학회지 Vol.14 No.4

The nucleotide sequence for a nuclear-encoded small subunit rDNA (SSU rDNA) was determined for 43 species of the class Dinophyceae, including harmful algae Cochlodinium polykrikoides and Gyrodinium aureolum. These sequences and data analyses were performed by parsimony, distances and maximum likelihood methods in PHYLIP (Phylogenetic Inference Package) version 3.573c. The species Noctiluca scintillans, Gonyaulax spinifern and Crypthecodinium cohnii occupied a basal position within the Dino- phyceae in our analyses. The genera Alexandrium and Symbiodinium were monophyletic (supported by a bootstrap value of >70%), whereas the genera Gymnedinium and Gyrodinium formed polyphyletic nodes, for which bootstrap support was strong (>70%) in the neighbor-joining and maximum likelihood methods except for the PHYLIP parsimony analysis (=59%). The sequence divergence between G. aureolum and G. dorsum/ G. galathenum was the largest at 7.4% (45 bp), whereas G. aureolum and G. mikimotoi showed an extremely low value of genetic divergence of 0.9% (5 bp). The genetic divergence between C. polykrikoides and G. aureolum was a low value of 5.2% (31 bp). In the phylogenetic analysis, the placement of G. aureolum and C. polykrikoides was closer to the genus Gymnodinium than to the genus Gyrodinium, which was supported by a moderate bootstrap value. 유해성 적조생물 Cochlodinium polykrikoides/Gyrodinium aurelum을 포함한 43 종류의 와편조류를 대상으로 SSU 부위 유전자를 분석했다. 유전자 염기서열에 의거한 상호 계통수는 parisomny, distance, maxium 방법으로 실행했다. Noctilura scintillans, Gonyaulax spinifera와 Crythecodinium cohnii 종들은 와편모조류 중 가장 유전적으로 먼 것으로 보였다. Alexandrium과 Symbiodinium 종간의 bootstrap는 70% 이상의 상호 단일 계통도를 보인 반면에, Gymnodinium과 Gyrodinium은 근립절약계수와 최대 유사도 방법에서 다형 계통도를 나타내었다. Gyroddinium aurelum과 G. dorsum/G. galathenum의 유전적 분화율은 7.4% (45 bp) 였고, G. aurelum과 G. mikimotoi 상호간에는 0.9% (5bp) 밖에 나타나지 않았다. 또한 C. polykrikoides와 G. aurelum도 5.2% (31bp)로 낮은 유전적 분화율을 보였다. 계통도를 분석한 결과 G. aureolum과 C. polykrikoides는 Gyrodinium 보다 Gymnodinim 속에 훨씬 더 근접하게 나타났다.

Genetic analysis of the purplish Washington clam (Saxidomus purpuratus Sowerby) of Korean coastal waters.

Cho, Eun-Seob,Seo, Young-Il,Suh, Young-Sang The Academy 2013 Journal of environmental biology Vol.34 No.3

<P>To investigate the genetic structure of the purplish Washington clam population, Saxidomus purpuratus Sowerby, in Korea. A portion of mitochondrial COI gene sequences (605 bp) for phylogenetic comparison was determined. Sequence analysis of 62 individuals collected from six regions revealed 13 haplotypes. Phylogenetic analysis using Phylogeny Inference Package (PHYLIP) subdivided the purplish Washington clam into two clades (termed clade A and B), weak supported groups (< 65 of bootstrap value). This haplotype subdivision was also in accordance with geographic separation; one each at Masan, Yeosu, Samcheonpo, Jubyeon and Geojedo, and the other at Sineju. Population genetic analysis subdivided these two population groups with a geographic distance (d = 0.431, p = 0.379). Furthermore, in the Sineju population, the maximum sequence divergence (2.67%) and minimum nucleotide diversity (0.0012426) were shown in which might be reflective of a relatively small population size and the geographical isolation of the population as compared with other populations. However, a very high migration rate (N(m) = 59.62-infinite) and a very low level of geographic distance (F(ST) = -0.076-0.055) were noted to exist among the South and East Sea populations, suggesting that individuals between populations should show a significantly active genetic mixing and migration regardless of geography. These findings allowed us to conclude that the purplish Washington clam populations occurring in the South and East Sea were formed with randomly dispersed individuals.</P>

코클로디니움 적조생물의 이용방안 연구: I. 점액질

Eun Seob Cho 한국자연보호학회 2016 한국자연보호학회지 Vol.10 No.1

For the last few decades, especially during summer Korean aquaculture industry has been huge economic loss due to massive blooms caused by Cochlodinium polykrikoides. Moreover, high dispersion speed along the coast and high cell densities (above 10,000 cells ml−1) of C. polykrikoides resuling in red tides for a longer duration (at least for 30 days). C. polykrikoides can be used as a prolific source of extracellular sulfated polysaccharides. Sulfated polysaccharides have showed strong antiviral properties against influenza virus. However, little has been investigated about industrial application of sulfated polysaccharides as a high valuable byproduct from C. polykrikoides. Current commercial microalgal biomass production systems are costly and require advanced instrumental and cultural facilities. From economic point of natural blooms of C. polykrikoides, therefore it is likely to be utilized as a cost-effective way of microalgal biomass production for commercial applications.