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Baffled fuel-storage container: parametric study on transient dynamic characteristics
Lee, Sang-Young,Cho, Jin-Rae,Park, Tae-Hak,Lee, Woo-Yong Techno-Press 2002 Structural Engineering and Mechanics, An Int'l Jou Vol.13 No.6
In order to ensure the structural dynamic stability of moving liquid-storage containers, the flow motion of interior liquid should be appropriately suppressed by means of mechanical devices such as the disc-type elastic baffle. In practice, the design of a suitable baffle requires a priori the parametric dynamic characteristics of storage containers, with respect to the design parameters of baffle, such as the installation location and inner-hole size, the baffle number, and so on. In this paper, we intend to investigate the parametric effect of the baffle parameters on the transient dynamic behavior of a cylindrical fuel-storage tank in an abrupt vertical acceleration motion. For this goal, we employ the ALE (arbitrary Lagrangian-Eulerian) kinematic description method incorporated with the finite element method.
A New High Yielding Rice Variety with Multi-resistance to Diseases "Sujinbyeo"
Sang Jong Lim,No Bong Park,Do Yeon Kwak,Heung Gu Hwang,Ho Yeong Kim,Un Sang Yeo,Sae Jun Yang,Jong Rae Kang,You Cheon Song,Jeom Sik Lee,Jae Ki Chang,Woon Goo Ha,Byeong Geun Oh 한국육종학회 2003 한국육종학회지 Vol.35 No.1
Sujinbyeo', a new mid-late maturing japonica rice variety (Oryza sativa L.), was developed by the rice breeding team of National Yeongnam Agricultural Experiment Station (NYAES) in 1999 and released in 2000. This variety originated from a three way cross
Sung-Sik Kang,Ui-Hyung Kim,Myung-Suk Lee,Seok-Dong Lee,Sun-Sik Jang,Ki-Yong Chung,Hyun-Joo Kim,Sang-Rae Cho 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
In this study, we examined number, motility and plasma membrane integrity of spermatozoa from six regions of epididymis in bull. Six testicles with epididymides were castrated from six bulls (mean±standard error, age of days = 441.3±9.6, body weight (kg) = 367±8.4, scrotal circumference (cm) = 30.7±0.4) at Hanwoo Research Institute, NIAS and transported to laboratory within 1 hour. Testicular weight, length, width and circumference were recorded. Epididymis in each bull was randomly used for recovery of spermatozoa. Epididymis was divided into six regions: efferent duct (ED), caput, corpus, proximal cauda (Pcauda), distal cauda (Dcauda) and vas deferens (VD). In experiment 1, we examined sperm number of each region of epididymis. Each region of epididymis contained different number of spermatozoa: ED (37.8±15.7 × 106cells/ml, 8.2%), caput (93.6±18.8 × 106cells/ml, 20.2%), corpus (33.0±8.5 × 106cells/ml, 7.1%), Pcauda (104.2±23.5 × 106cells/ml, 22.5%), Dcauda (180.5±32.5 × 106cells/ml, 39.0%) and VD (14.0±5.0 × 106cells/ml, 3.0%). In experiment 2, sperm motility of each epididymal region was examined by computer assisted sperm analysis (SCA, MicroOptic) system. Sperm motility was divided into 4 groups (fast progressive, slow progressive, non-progressive and immotile) based on WHO guideline. Percentages of fast progressive of Pcauda and Dcauda (11.0±2.3 and 15.4±3.6%) were significantly higher than that of ED, Caput, Corpus and VD which is 0.1±0.1, 1.5±0.6, 1.9±0.7 and 0.3±0.2%, respectively (p<0.05). In experiment 3, percentage of intact plasma membrane spermatozoa of each regions were examined by hypoosmotic swelling test. Percentages of intact plasma spermatozoa were not significantly different among six regions of epididymis: ED, caput, corpus, Pcauda, Dcauda and VD which is 68.0±8.6, 74.0±5.3, 68.5±6.2, 70.8±5.5, 71.0±5.8 and 64.6±10.8%, respectively. In conclusion, in the present study, we found out distribution, motility and plasma membrane integrity of spermatozoa from six regions of epididymis in Hanwoo bull. These results will be contributed to basic research about spermatozoa transportation and characters in epididymis of bull.
Sang-Rae Cho,Sung-Sik Kang,Ui-Hyung Kim,Seok-Dong Lee,Myung-Suk Lee 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
In this study, we examined sperm penetration and blastocyst developmental rate of oocytes to determine fertilizability of cauda epididymal spermatozoa in Hanwoo bull. One testicle with epididymides were castrated from one Hanwoo bull (14 months of age) and transported to laboratory. Spermatozoa recovered from cauda epididymis by mincing with semen extender (Optixcell, IMV, France) and cryporeserved in liquid nitrogen tank until use. As control, frozen Hanwoo semen was used. Cumulus oocyte complexes (COCs) were collected from follicles (2-8 mm) of slaughtered ovaries and 10 to15 COCs were matured in 50μl droplet with M-199 media supplemented with 10% fetal bovine serum, 10μg/ml FSH, 10μg/ml LH, 10μg/ml EGF for 22 to 24 hours in a humidified atmosphere of 5% CO2 in air. After maturation of COCs, matured COCs were co-incubated with cauda epididymal spermatozoa in 100μl droplet in modified Brackett and Oliphant media supplemented with 2.5 mM theophylline for 12 or 18 hours under 5% CO2 in air. Sperm concentration was adjusted to 5 × 106cells/ml. After IVF for 18 hours, presumptive zygotes were cultured in modified synthetic oviductal fluid with 1mM glutamine, 12 essential amino acids, 10 μg/ml insulin under 5% CO2, 5% O2 in air. In experiment 1, we examined sperm penetration rate at 12 hours of IVF of frozen-thawed epididymal sperm. Total penetration rate among cauda epididymis and control were not significantly different (mean±standard error, cauda epididymis and control vs. 49.7±11.3 and 54.4±12.8%) In experiment 2, cleavage and blastocyst development rate were evaluated at day 2 and day 8 after IVF for 18 hours. Cleavage rate among cauda epididymis and control was similar different (cauda epididymis and control vs. 81.2±3.4 and 82.7±2.5%). However, blastocyst developmental rate of cauda epididymis group was significantly higher than that of control group (cauda epididymis and control vs. 24.4±1.6 and 12.2±2.8%, p<0.05). In conclusion, cauda epididymal spermatozoa in Hanwoo bull has high fertilizability and embryo development. Cauda epididymal sperm can be used as an alternative to ejaculated frozen sperm in vitro.
Therapeutically Targeting Neuroinflammation and Microglia after Acute Ischemic Stroke
Lee, Youngjeon,Lee, Sang-Rae,Choi, Sung S.,Yeo, Hyeon-Gu,Chang, Kyu-Tae,Lee, Hong J. Hindawi Publishing Corporation 2014 BioMed research international Vol.2014 No.-
<P>Inflammation has a pivotal role in the pathogenesis of ischemic stroke, and recent studies posit that inflammation acts as a double-edged sword, not only detrimentally augmenting secondary injury, but also potentially promoting recovery. An initial event of inflammation in ischemic stroke is the activation of microglia, leading to production of both pro- and anti-inflammatory mediators acting through multiple receptor signaling pathways. In this review, we discuss the role of microglial mediators in acute ischemic stroke and elaborate on preclinical and clinical studies focused on microglia in stroke models. Understanding how microglia can lead to both pro- and anti-inflammatory responses may be essential to implement therapeutic strategies using immunomodulatory interventions in ischemic stroke.</P>
( Sang Jin Lee ),( Na Rae Kim ),( In Sook Choi ) 경남대학교 신소재연구소 2012 신소재연구 Vol.24 No.-
In order to investigate the consolidating mechanism of the weathered ston monuments containing clay minerals, the consolidating effects were studied by applying 9 types of the ethylsilicates consolidants onto the shale containing clay minerals. XRD and SEM were used to find the influence of the clay minerals. The XRD results showed that the lattice spaces of the clay minerals was changed by infiltrating the consolidants. Through the investigation of the reaction mechanism between the stone samples containing clay minerals(smectites) and the consolidants, both the expansion and the shrinkage of the lattice space were found. On the contrary, in case the swelling inhibitor were applied to the stone samples containing the clay minerals, the changes of the lattice space were reduced.