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Kyungmun Kim,Sang hyeon Kim,Jea ho Lee,Seung hee Han,Young ho Kim,Si hyeock Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.10
Honey bee swarming is a natural phenomenon that occurs when the colony encounters changes in the in-hive (i.e. population size and queen condition) and environmental conditions. To better understand the molecular basis of swarming, we conducted the transcriptomic profiles of worker bees between before swarming [pre-swarming colony (PSC)] and after swarming [swarming group (SG) and remaining group (RG)]. Based on the gene set enrichment analysis (GSEA), we predicted the biological processes associated with swarming. In addition, we analyzed the composition of cuticular hydrocarbons (CHCs) by gas chromatography-mass spectrometry and compared their profiles between different bee groups. GSEA results showed that there were a little differences between PSC and RG while many of the pathways related with metabolism and protein processing were down regulated in SG relative to PSC and RG. CHCs profiling revealed a similar CHCs composition between PSC and RG but some differences in CHCs composition (i.e. heneicosane, octacosane, octacosanol) were detected between SG and RG. These differences in gene pathway and CHC composition were discussed with respect to physiological changes and social communication.
Kyungmun Kim,Sang Hyeon Kim,Yun Sang Cho,Si Hyeock Lee 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.04
Small hive beetle (Aethina tumida) (SHB) is an invasive species to most northern hemisphere countries, including Korea. In an attempt to obtain basic information for efficient management of SHB, genes encoding conventional insecticide targets [voltage-sensitive sodium channel α-subunit (VSSC) and acetylcholinesterase (AChE)] were annotated and characterized following the analysis of whole transcriptomes of adults and larvae. A single VSSC gene was identified but no apparent mutations associated with pyrethroid resistance were detected. Genes encoding two AChEs (AtAChE1 and AtAChE2) were identified from the SHB transcriptome. AtAChE1 was determined to be the main catalytic enzyme, thereby being a toxicologically more relevant target. No apparent mutations associated with resistance to organophosphorus and carbamate insecticides was identified in the AtAChE1 gene, whereas the S238G mutation, originally identified from the Colorado potato beetle, was detected in the AtAChE2 gene.
Kyungmun Kim,김주현,Young Ho Kim,Si Hyeock Lee 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1
Tropilaelaps mercedesae (Acari: Laelapidae) is an ectoparasite of the Western honey bee Apis mellifera and has become a major threat to the apiculture in Asia, including Korea. To establish gene resources of T. mercedesae, the whole transcriptome of mixed developmental stages of mites was analyzed by RNA sequencing. An mRNAfocused library was generated from total RNA extracted from the mixed stages (adults, nymphs and larvae) of T. mercedesae using the TruSeq RNA Library Preparation kit and sequenced using the HiSeq 2000 platform. A total of 59,041,198 reads were obtained with 85% Q30 value. Trimmed sequence data was de novo assembled into 64,868 contigs with an average sequence length of 878 bp. Transcriptome annotation to the NCBI nonredundant database resulted in a total of 14,336 Blast hits (22.1%). Functional analysis demonstrated that 11,919 contigs were grouped into 45 Gene Ontology (Conesa et al.) terms, and 1226 contigs were assigned to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Based on the transcriptome dataset, a large number of contigs associated with binding, cellular process and catalytic activity were identified. In order to provide basic information for efficient management of potential resistance to fluvalinate, a pyrethroid acaricide most widely used for the control of T. mercedesae, the gene encoding the voltage-sensitive sodium channel α-subunit (vssc), which is the molecular target of fluvalinate, was annotated and characterized Tropilaelaps mercedesae (Acari: Laelapidae) is an ectoparasite of the Western honey bee Apis mellifera and has become a major threat to the apiculture in Asia, including Korea. To establish gene resources of T. mercedesae, the whole transcriptome of mixed developmental stages of mites was analyzed by RNA sequencing. An mRNAfocused library was generated from total RNA extracted from the mixed stages (adults, nymphs and larvae) of T. mercedesae using the TruSeq RNA Library Preparation kit and sequenced using the HiSeq 2000 platform. A total of 59,041,198 reads were obtained with 85% Q30 value. Trimmed sequence data was de novo assembled into 64,868 contigs with an average sequence length of 878 bp. Transcriptome annotation to the NCBI nonredundant database resulted in a total of 14,336 Blast hits (22.1%). Functional analysis demonstrated that 11,919 contigs were grouped into 45 Gene Ontology (Conesa et al.) terms, and 1226 contigs were assigned to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Based on the transcriptome dataset, a large number of contigs associated with binding, cellular process and catalytic activity were identified. In order to provide basic information for efficient management of potential resistance to fluvalinate, a pyrethroid acaricide most widely used for the control of T. mercedesae, the gene encoding the voltage-sensitive sodium channel α-subunit (vssc), which is the molecular target of fluvalinate, was annotated and characterized.