Spawning Behavior, Egg Development, Larvae and Juvenile Morphology of Hyphessobrycon eques (Pisces: Characidae) Characidae Fishes

Jae-Min Park,Na-Ri Kim,Kyeong-Ho Han3,Ji-Hyeong Han,Maeng-Hyun Son,Jae-Kwon Cho 한국발생생물학회 2014 발생과 생식 Vol.18 No.4

Hyphessobrycon eques is a famous fish for ornamental fish market and aquarium. They are inhabit in regions of Amazon and Paraguay River basin. Serpae fishs were investigated 2–3 males are chased to female, and then males attempted to simulate the females abdomen. After fertilization, eggs were kept in incubators at 28oC. The fertilized eggs had adhesive and demesal characteristics and had a mean diameter of 0.92 ± 0.01 mm. Larvae hatched at 16 hrs post fertilization. The hatched larvae averaged 2.90 ± 0.16 mm in total length (LT). Complete yolk sac resorption and mouth opening occurred on the third day post hatching. At 45 days post hatching, the larvae were 12.5 ± 1.60 mm LT and had reached the juvenile stage.

버들치(Phoxinus oxycephalus) 仔稚魚의 골격 발달

한경호,노병율 여수대학교 2001 論文集 Vol.16 No.-

Twenty individuals of the Phoxinus oxycephalus (Sauvage et Dabry) were caught in Kyeongho River, Kyeongsangnam-do and were reared in the laboratory. During the rearing, they spawned 5 times in April, 1998, and their larvae and juveniles were reared. The osteological development of Phoxinus oxycephalus was studied based on them. At the cranium, parasphenoid was firstly ossified in the larvae (6 days after hatching), mean 6.44㎜ in total length (TL), and ossification of the cranium was completed in the juveniles (80 days after hatching), means 18.76㎜ in TL. At the visceral, opercle and dentary were firstly ossified in the larvae (13 days after hatching), mean 6.82㎜ in TL, and ossification of the visceral was completed in the juveniles (80 days after hatching). The ossification of the vertebral columns began posteriorly and was completed in the juveniles (80 days after hatching). The caudal skeleton was firstly ossified in the larvae (16 days after hatching), mean 7.02㎜ in TL and comleted in the larvae (44 days after hatching), 13.72㎜ in TL.

넙치(Paralichthys olivaceus)의 초기생활사 : ll. 仔稚魚의 골격발달 ll. Osteological Development of Larvae and Juveniles

韓景鎬,金容億 여수대학교 1998 論文集 Vol.13 No.2

넙치 친어를 1995년 5월 19일 오전 8시에 경상북도 포항시 종묘배양장에서 건식법으로 인공수정한 후 수정난에서 孵化한 仔魚를 사육하면서 孵化後 50일까지 매일 10개체를 무작위 추출하여 5% 중성 formalin에 고정시킨 뒤 표본을 염색하여 성장에 따른 골격의 정상적인 發達 過程을 관찰하였다. 1. 孵化後 6∼7일째인 全長 3.75∼3.87㎜의 仔魚에서 額骨과 頭蓋骨이 가장 먼저 骨化되기 시작한다. 2. 脊椎骨은 腹椎骨이 먼저 發達하여 尾椎骨 쪽으로 骨化가 진행되며 각 椎骨에 대응하는 神經棘과 血管棘이 椎體보다 먼저 骨化한다. 3. 腰帶骨은 肩帶部의 鎖骨 下部에 접착되어 있다. 4. 담기골은 脊椎骨과 각 지느러미 연조가 거의 완성된 후에 骨化한다. 5. 尾骨의 尾部棒狀骨은 앞의 椎體와 거의 동시에 骨化하기 시작한다. 6. 모든 내부 골격은 全長 14.60∼20.90㎜ 전후(孵化後 40∼50일째)에 肩帶骨과 腰帶骨이 완전하게 骨化됨으로서 모두 완성된다. The flounder, Paralichthys olivaceus (Temminck et Schlegel) used in the experimental were caught at Pohang brook was performed in May 19, 195 and hatched larvae were reared for 50 days to describe the osteological development of larvae and juveniles. Ossification of the cranium took place at 4.75㎜ of mean total length(MTL) in frontal, supraoccipital, parasphenoid and sphenotic. Ossification of the viseral skeleton occurred in areas where active movements of bones were required, notable in the parts for feeding(premaxillary, dentary, articular) and respiration(opercle, prepercle). Vertebrate began to develop from the anterior end and to ossify posteriorly. Neural and haemal spines of vertebrae ossified always prior to the corresponding centra. In jaw bones, maxillary and a part of dentary appeared first at 4.75㎜ of MTL and attained a fundamental structure at 6.47㎜ of MTL. Ossification of all bones was nearly completed at 16.80㎜ in MTL(45 day after hatching).

넙치(Paralichthys olivaceus)의 초기생활사에 관한 연구 I. 난발생과정 및 仔稚魚의 形態發達

韓景鎬,金容億 여수대학교 1997 論文集 Vol.11 No.2

Artificial fertilization of the flounder, Paralichthys olivaceus(Temminck et Schlegel) caught at Pohang brook was performed in May 19, 1995 and hatched larvae were reared for 50 days to describe the development of eggs and the morphogenesis of larvae and juveniles. The egg is bouyant, colorless and spherical in shape, measuring 0.90~0.94㎜ in diameter(mean: 0.92㎜). Hatching in the indoor tank with 17.60℃ in mean water temperature started from the 50 hours after fertilization. Newly-hatched larvae were measured 2.40~2.53㎜ in total length(TL, mean:2.49㎜), and mouth and anus were not yet open. Melanophores and xanthophores were present on the yolk and membrane fin, and on the dorsal and ventral part of the caudal region. Five days post-hatch larvae transformed to postlarval stage and they were 3.84~4.50㎜ in TL(mean: 4.20㎜). As yolk sac were completely resorbed, mouth and anus were open, and they fed rotifer, Branchionus plicatilis actively. In 18 days after hatching, the larvae grew to 7.85㎜ in TL, number of elongated dorsal fin rays 6~7, and membrane of these hypertrophied rays densely pigmented. Individuals of 11.40~13.25㎜ in TL(30 days after hatching) are regared to have attained to the juvenile stage. All the fins were well formed with complete set of fin rays(D. 76~83; A. 60~65; P. 14~16; V.6; C.18~19).

저질에 따른 바지락(Ruditapes philippinarum)의 염분내성

한경호,오성현,장선익,이우범,이원교 國立 麗水大學校 環境問題硏究所 2000 環境硏究論文集 Vol.3 No.-

Salinity tolerance of the manila clam, Ruditapes philippinarum in Gang-jin Bay on the from May to June was investigated in two different types of sedimentary composition. In the experimental groups of sandy-mud, the survival and infiltration rate were good at 20~40ppt, but all the manila clam, Ruditapes philippinarum died at more than 50ppt and less than 10ppt before the experiment finish. In the experimental groups of muddy, the survival and infiltration rate were the best at 25~35ppt and then 20ppt, 40ppt. All the manila clam, Ruditapes philippinarum died at more than 45ppt and less than 15ppt. The survival rate in the experimental groups of sandy-mud was higher than muddy ones, and the infiltration rate was the highest at 20~35ppt in the both of two experimental groups. In the sand-mud, the manila clam, Ruditapes philippinarum infiltrated half of their shells but in the muddy, they infiltrated more then half of their shells.

고흥반도 연안에 분포하는 浮遊性 卵·仔稚魚 종조성의 계절변동

한경호,신영호,황동식 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.1

전라남도 고흥군 녹동연안에서 2000년에 계절별로 총 4회에 걸쳐 만조시 채집된 浮遊性 卵·仔種魚의 종조성 및 양적변동을 조사하였다. 조사기간 동안 채집된 浮遊性 卵은 총 8개 분류군이 출현하여 7개 분류군은 種 수준까지, 1개 분류군은 기타난으로 동정되었다. 그 중에선 멸치(Engraulis japonica)가 전체 출현량의 52.1%를 차지하여 가장 우점하였으며, 다음으로 기타난이 13.2%, 주둥치(Leiognat husnuchalis)가 12.3% 보리멸(Sillago sihama)이 8.6%, 앨퉁이(Maurolicus muelleri) 4.0%, 전어 (Konosirus punctatus) 1.0%, 참서대(Cynoglossus joyneri) 0.2%을 차지하였다. 仔種魚는 총 8目 26科 33屬 37개 분류군의 仔種魚가 출현하여,33개 분류군은 種 수준, 2개 분류군은 屬수준, 2개 분류군은 科 수준까지 동정되었다. 조사기간 동안 출현한 仔種魚 중에서 멸치가 31.7%를 차지하여 최우점하였으며, 망둑어科(Gobiidae)에 속하는 어류는 46.2%, 베도라치(Pholis nebulosa)와 실고기(Syngnathus schlegeli)는 각각 5.4%와 2.3%를 차지하였다. 조사기간 동안 출현한 仔種魚 중에서 이들 4개 분류군이 차지한 비율은 85.6%를 차지하였다. The ichthyoplankton were sampled during four different months (2000) to study their seasonal variations and species composition off Kohung Peninsula. During the study, the fish eggs were identified to eight taxa, seven of which were identified to species level, and one identified to order. The most dominant species. Engraulis japonicus, accounted for 52.1% of the total fish eggs, followed by Leiognathus nuchalis(4.0%), Sillago sihama(8.6%), Maurolicus muelleri(8.6%), Sardinops melanostictus(4.0%), Konosirus punctatus(1.0%), Cynoglossus joyneri(0.2%). The collected larvae and juveniles were identified to 37 taxa (33 genera, 26 families, 8 orders). Of these, 33 were identified to species level, and two were identified to genus and family level, respectively. The dominant species, Engraulis japonicus, accounted for 31.7% of the total larvae and juveniles; it was followed by Gobiidae(46.2%), Pholis nebulosa(5.4%) and Syngnathus schlegeli(2.3%). These four taxa constituted 85.6% of the total collected larvae and juveniles.

복섬(Takifugu niphobles)자치어의 골격발달

한경호,오성현,서원일 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.3

복섬의 부화 직 후 자어는 평균 전장 2.14 ㎜로 골격의 골화가 전혀 일어나지 않았으며, 부화 후 1일째 평균 전장 2.38 ㎜의 자어는 두개골에 복추골, 섭이와 관련하는 턱부분에 전상악골과 치골이 먼저 골화가 시작되었다. 부화 후 10일째 자어는 평균 전장 4.24㎜로, 두개골에 전이골과 익이골이 골화되었고, 내장골의 새개부에는 하새개골, 간새개골이 골화하여 새개부가 완성되고, 견대부의 후쇄골이 골화하기 시작하였다. 부화 후 28일째 후기자어의 평균 전장은 7.60㎜로 두개골에 상이골과 상후두골이 골화하여 두개골이 완성되었고, 척추골, 미골 및 담기골이 거의 동시에 골화되었다. 부화 후 35일째 치어는 평균 전장이 8.60㎜로 구개부에 후익상골이 골화하여 내장골이 골화가 완료되므로서, 모든 골격의 골화가 완성되었다. Eggs of Takifugu niphobles (Jordan et Snyder) collected on the shores of Dolsan, Yeosu, Korea, in July 1998, were artificially hatched and the larvae reared in the laboratory to investigate osteological development of larvae and juveniles. The newly hatched larvae attained a mean size of 2.38 ㎜ in total length (TL) and had an ossified dentary, premaxillary and clavicle at this stage. At 10 days after hatching, the larvae (mean 4.24 ㎜ in TL) had ossified prootic, pterotic, subopercle, and interopercle, and the postclavicle had begun to ossify. The ossification of the cranium, vertebrae, caudal skeleton and pterygiophore was complete in the larvae (mean 7.60 ㎜ in TL) at 28 days, and ossification of all bones was complete in the juveniles (mean 8.61 ㎜ in TL) at 35 days.

Scartella cristata (갈기베도라치: 국명신칭) 자치어의 형태발달

한경호,황동식 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.1

본 연구는 2001년 5월부터 10월에 광양만, 가막만 및 여수시 돌산도 연안에서 채집한 Scartella cristata의 발육단계에 따른 자치어의 형태를 관찰하였다. 채집돈 Scartella cristata 자치어는 총 235개체로 주로 7월과 8월에 각각 78개체, 93개체가 채집되어 가장 많았고, 체장범위는 2.53~15.85㎜였다. 본 종의 자치어들은 몸의 형태, 체형, 계수형질 및 흑색소포의 분포 상태가 Okiyama(1998)가 보고한 Scartella cristat의 결과와 일치하였다. Scartella cristata는 한국 미기록 어종으로 머리부분에 여러갈래의 피질돌기가 갈기 모양으로 형성되어 있는 특징에 근거하여 “갈기베도라치”로 국명을 신칭하였다. 평균 체장 2.57㎜의 자어는 난황이 완전하게 흡수되어 있고, 근절은 28~30개였다. 평균 체장 5.95㎜ 이상의 개체에서 완전하게 위로 굽어져 있었다. 평균 체장 10.50㎜ 전후의 개체들은 모든 지느러미 줄기가 정수에 달하여 치어기가 이행하였고, 평균체장 12.20㎜ 치어들은 코 부분과 눈 윗부분에 갈기모양의 피질돌기가 형성되기 시작하였다. The development of larvae and juveniles of the blenniid fish, Scartella cristata were studied based on the specimens collected in coastal waters of Yosu, Korea from May to October, 2001. 235 individuals of the species were collected during the study, which were 2.53-15.85㎜ in standard length. Body form, meristic characters and distribution of melanophores coincided with those of Scartella cristata described by Okiyama (1988), which was not revealed from Korean. Therefore, we proposed it's new Korean name as "Gal-gi-be-do-ra-chi". Yolk was completely absorbed when the larvae was about 2.57 mm in average standard length (ASL), with 28-30 myomeres. Flexion of the notochord started when the postlarvae was about 5.12 ㎜ in ASL and finished about 5.95 mm in ASL, respectively. The larvae had reached at the juvenile stage when 10.50㎜ in ASL and all the fins were completed. The cirri and fleshy crests were begun to appear on the snout and upper region of eye respectively when 12.20㎜ in ASL.

한국産 참복屬(참복科) 魚類의 分類學的 硏究

韓景鎬,金容億 여수대학교 1998 論文集 Vol.12 No.2

The taxonomic revision of the Genus Takifugu(Tetraodontidae) was conducted based on the specimens collected from the coasts of Korea Peninsula from July, 1990 to August, 1994. The species relationships of 15 species of the genus Takifugu were studied based on external morphological characters. Of these, 3 species are new to the Korean fish fauna. They are Takifugu bimaculatus (Richardson). Takifugu snyderi(Abe) and Takifugu sp... Based on spinous scales(prickles), the species of T. alboplumbeus, T. rubripes, T. chinensis, T. xanthopterus, T. niphobles, T. stictonotus, T. flavidus, T. bimaculatus have dorsal and ventral region, and those of T. obscurus have dorsal, ventral and opercular region, while T. snyderi, T. vermicularis, T. prophyreus have no spinous scales. Takifugu stictonotus have larger ventral spinous scales than dorsal ones. Takifugu alboplumbeus and T. poecilonotus were considered ancestral group because of the ancestral character of the vertebrae, dorsal and anal fin ray and pterygiophors.

참복과 어류 1미기록종, Takifugu oblongus (폭포무늬복: 국명신칭)의 형태 및 골격

한경호,김춘철,윤성민 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.3

이 실험에 사용된 Takifugu oblongus (Bloch)는 2001년 7월에 동중국해에서 중저층 트롤로 채집된 5마리(체장 195.0∼265.3 ㎜)로 표본의 형질을 조사하기 위하여 외부형태를 관찰한 후에 계수·계측하였으며, 골격의 형태는 삶아서 채골하여 관찰하였다. T. oblongus는 등지느러미 줄기수는 12∼13개이며, 뒷지느러미 줄기수는 10∼11개, 가슴지느러미 줄기수는 16개, 꼬리지느러미 줄기수는 11개이다. 척추골은 19∼20개(미부봉상골 포함), 새조골은 6개이다. 그리고 체색은 등쪽에서 배쪽으로 폭포무늬의 황색의 띠가 가로줄로 내려와 있었고, 등지느러미 바로 앞쪽의 경우 띠가 한선으로 연결된 점이 특징이다. 이러한 특징으로 한국 미기록 어종인 T. oblongus을 "폭포무늬복"로 신칭하였다. The oblong toadfish, Tukifugu oblongus (Bloch), of the family Tetraodontidae was studied for the first time in Korea. The specimens were collected by an intermediate-bottom-layer trawl in East China Sea in July 2001. The species is characterized by brownish above with pale spots (waterfall design), yellowish white below, narrow dark bars on sides, teeth fused into a beaklike dental plate with a median suture giving two teeth in each jaw, 12 to 13 dorsal fin rays, 16 pectoral fin rays, 10 to 11 anal fin rays, 11 caudal fin rays, 19 to 20 vertebrae and 6 branchiostegal rays. A new Korean name "Pok-po-moo-nyi-bok" is proposed for the species.