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- 저자 : Kangping Liu (검색결과 2 건)
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Autophagy of bovine mammary epithelial cell induced by intracellular Staphylococcus aureus
Na Geng,Kangping Liu,Jianwei Lu,Yuliang Xu,Xiaozhou Wang,Run Wang,Jianzhu Liu,Yongxia Liu,Bo Han 한국미생물학회 2020 The journal of microbiology Vol.58 No.4
Bovine mastitis is a common disease in the dairy industry that causes great economic losses. As the primary pathogen of contagious mastitis, Staphylococcus aureus (S. aureus) can invade bovine mammary epithelial cells, thus evading immune defenses and resulting in persistent infection. Recently, autophagy has been considered an important mechanism for host cells to clear intracellular pathogens. In the current study, autophagy caused by S. aureus was detected, and the correlation between autophagy and intracellular S. aureus survival was assessed. First, a model of intracellular S. aureus infection was established. Then, the autophagy of MAC-T cells was evaluated by confocal microscopy and western blot. Moreover, the activation of the PI3K-Akt-mTOR and ERK1/2 signaling pathways was determined by western blot. Finally, the relationship between intracellular bacteria and autophagy was analyzed by using autophagy regulators (3-methyladenine [3-MA], rapamycin [Rapa] and chloroquine [CQ]). The results showed that S. aureus caused obvious induction of autophagosome formation, transformation of LC3I/II, and degradation of p62/SQSTM1 in MAC-T cells; furthermore, the PI3K-Akt-mTOR and ERK1/2 signaling pathways were activated. The number of intracellular S. aureus increased significantly with autophagy activation by rapamycin, whereas the number decreased when the autophagy flux was inhibited by chloroquine. Therefore, this study indicated that intracellular S. aureus can induce autophagy and utilize it to survive in bovine mammary epithelial cells.
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Gui-Xin Wang,Kangping Yan,Rui Liu,Miao Chen,Hanchang Kang,Xiuli Li 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.8
A novel route was designed to synthesize LiFePO4/C composites by using the Fe1.5P byproduct, mixed lithium salts, and permeated oxygen from air via a rheological phase method. The reaction process was investigated with various techniques. When the calcining time was increased from 10 to 30 h, the gradual formation of olivine structure was observed. The growth kinetics of the crystals was analyzed. SEM and TEM results indicated the as-synthesized LiFePO4 was constituted of small spheres covered with carbon particles. The discharge capacity of the LiFePO4/C composite prepared at ~700 oC for ~25 h could reach 139.7 mAh g−1 and still remained 130.2 mAh g−1 after 15 cycles at 0.2 C rate, comparable to that of the reported LiFePO4/C composite using conventional methods. Cyclic voltammogram confirmed the LiFePO4/C composite had a high purity and good lithium ion insertion/desertion redox behavior.
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