http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Development of a Magnetic-field Stimulation System for Cell Cultures in situ
G. Dominguez,S. Arias,Jose L. Reyes,Pablo Rogeli 한국자기학회 2017 Journal of Magnetics Vol.22 No.2
The effects of exposure to an extremely low-frequency magnetic field (25 ㎐ 20G) on animal cells have been studied. In some reports, stimulation was performed for fixed frequency and variations in magnitude; however, animal-cell experiments have established that both parameters play an important role. The present work undertook the modeling, simulation, and development of a uniform-magnetic-field generation system with variable frequency and stimulation intensity (0-60 ㎐, 1-25G) for experimentation with cell cultures in situ. The results showed a coefficient of variation less than 1% of the magnetic-field dispersion at the working volume, which is consistent with the corresponding simulation results demonstrating a uniform magnetic field. On the other hand, long-term tests during the characterization process indicated that increments of only 0.4℃ in the working volume temperature will not be an interfering factor when experiments are carried out in in situ cell cultures.
Kim, Joonki,Jung, Jae-Hoon,Reyes, Jose L.,Kim, Youn-Sung,Kim, Sun-Young,Chung, Kyung-Sook,Kim, Jin A.,Lee, Minsun,Lee, Yoontae,Narry Kim, V.,Chua, Nam-Hai,Park, Chung-Mo Wiley (Blackwell Publishing) 2005 The Plant journal Vol.42 No.1
<P>Class III homeodomain-leucine zipper proteins regulate critical aspects of plant development, including lateral organ polarity, apical and lateral meristem formation, and vascular development. ATHB15, a member of this transcription factor family, is exclusively expressed in vascular tissues. Recently, a microRNA (miRNA) binding sequence has been identified in ATHB15 mRNA, suggesting that a molecular mechanism governed by miRNA binding may direct vascular development through ATHB15. Here, we show that miR166-mediated ATHB15 mRNA cleavage is a principal mechanism for the regulation of vascular development. In a gain-of-function MIR166a mutant, the decreased transcript level of ATHB15 was accompanied by an altered vascular system with expanded xylem tissue and interfascicular region, indicative of accelerated vascular cell differentiation from cambial/procambial cells. A similar phenotype was observed in Arabidopsis plants with reduced ATHB15 expression but reversed in transgenic plants overexpressing an miR166-resistant ATHB15. ATHB15 mRNA cleavage occurred in standard wheat germ extracts and in Arabidopsis and was mediated by miR166 in Nicotiana benthamiana cells. miR166-assisted ATHB15 repression is likely to be a conserved mechanism that regulates vascular development in all vascular plants.</P>