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Effects of Porcine Seminal Plasma in In Vitro Culture of Embryos
JoonHo Moon,SuJin Kim,JungTaek Kang,SolJi Park,JiYei Choi,JiHyun Lee,Islam M. Saadeldin,HyunJu Oh,JungEun Park,MinJung Kim,GeonA Kim,Eun Jung Park,Jin Choi,Goo Jang,Byeong Chun Lee 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s
Semen can be divided into two parts. One is cellular part which contains sperms the other is liquid part which is called by seminal plasma. The seminal plasma is a nutritive and protective medium for the sperms. Fructose, which is major energy source, is supplied to sperms swim to female oocyte. Alkalic property protects sperms from hostile environment of female reproductive organ. Also, seminal plasma induces tolerance to preexisted immune cells, and changes intra-uterine environment to better conditions for fertilized embryos to implant. However, the effects of seminal plasma in in vitro culture of fertilized embryos are unclear. Second fraction of fresh semen was obtained from a normal farm pig. The semen was centrifuged to remove sperms, and then supernatant was filtrated. The filtered seminal plasma was stored in - 30℃. In this study, electrically activated and chemically activated porcine embryos were employed to investigate the developmental rate after 2 hours treatment of none, 0.1%, 0.5%, and 1% seminal plasma in culture media by two days of activation. Both electrically and chemically activated embryos, cleavage rate and cell numbers of blastocysts were not significant difference within four groups. Blastocyst formation rate of electrically activated embryos also did not show significant difference within any groups. However 0.1% seminal plasma treatment group showed significantly increase of blastocyst formation rate in chemically activated group (None; 24.8%, 0.1%; 31.7%, 0.5%; 19.4, and 1%; 16.5%, respectively. p<0.05).
Effects of Porcine Seminal Plasma in In Vitro Culture of Embryos
JoonHo Moon,SuJin Kim,JungTaek Kang,SolJi Park,JiYei Choi,JiHyun Lee,Islam M. Saadeldin,HyunJu Oh,JungEun Park,MinJung Kim,GeonA Kim,Eun Jung Park,Jin Choi,Goo Jang,Byeong Chun Lee 한국동물번식학회 2012 Reproductive & developmental biology Vol.36 No.2
Oh, Daeyoung,Choi, Mi Ran,Han, Dal Mu Ri,Chai, Young Gyu,Choi, Joonho Wolters Kluwer Health | Lippincott Williams Wilkin 2014 NEUROREPORT - Vol.25 No.17
Fluoxetine, a serotonin-selective reuptake inhibitor, exerts antidepressant and antianxiety effects on major depressive and anxiety disorders. Previous studies suggest that treatment with fluoxetine influences the expression of various proteins that are involved in proliferation, differentiation, and apoptosis in the neuronal cells of the brain. However, many aspects of the molecular pathways that modulate antidepressant action are not well understood. Here, with the aim of identifying proteins involved in antidepressant action, we examined the protein expression profile of human embryonic carcinoma (NCCIT) cells in response to fluoxetine treatment using proteomic techniques such as two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). We found several upregulated and downregulated proteins in fluoxetine-treated NCCIT cells, and then biochemically confirmed the increased expression of heat shock protein 90 and 14-3-3&epsis;, which play an essential role in many cellular mechanisms including cell cycle control and other signaling pathways. Our data suggest that the regulated expression of heat shock protein 90, 14-3-3&epsis;, and other identified proteins may be associated with the therapeutic action of fluoxetine.
Oh, Yangkyun,Yoon, Sung-Eun,Zhang, Qi,Chae, Hyo-Seok,Daubnerová,, Ivana,Shafer, Orie T.,Choe, Joonho,Kim, Young-Joon Public Library of Science 2014 PLoS biology Vol.12 No.10
<▼1><P>A ligand of the sex peptide receptor maintains sleep stability and homeostasis by inhibiting wakefulness-promoting neurons in <I>Drosophila</I>.</P></▼1><▼2><P>Sleep, a reversible quiescent state found in both invertebrate and vertebrate animals, disconnects animals from their environment and is highly regulated for coordination with wakeful activities, such as reproduction. The fruit fly, <I>Drosophila melanogaster</I>, has proven to be a valuable model for studying the regulation of sleep by circadian clock and homeostatic mechanisms. Here, we demonstrate that the sex peptide receptor (SPR) of <I>Drosophila</I>, known for its role in female reproduction, is also important in stabilizing sleep in both males and females. Mutants lacking either the SPR or its central ligand, myoinhibitory peptide (MIP), fall asleep normally, but have difficulty in maintaining a sleep-like state. Our analyses have mapped the SPR sleep function to <I>pigment dispersing factor</I> (<I>pdf</I>) neurons, an arousal center in the insect brain. MIP downregulates intracellular cAMP levels in <I>pdf</I> neurons through the SPR. MIP is released centrally before and during night-time sleep, when the sleep drive is elevated. Sleep deprivation during the night facilitates MIP secretion from specific brain neurons innervating <I>pdf</I> neurons. Moreover, flies lacking either SPR or MIP cannot recover sleep after the night-time sleep deprivation. These results delineate a central neuropeptide circuit that stabilizes the sleep state by feeding a slow-acting inhibitory input into the arousal system and plays an important role in sleep homeostasis.</P></▼2><▼3><P><B>Author Summary</B></P><P>Sleep is a common trait in animals, from insects to mammals, and it needs to be coordinated with other critical activities such as feeding and reproduction. However, the mechanisms by which this is achieved are not fully understood. The fruit fly <I>Drosophila melanogaster</I> has become a key model organism for sleep research and it has been shown that reproduction is one of the factors that can modulate sleep in these animals. Researchers have observed that mating reduces the daytime sleep of female flies and shown that the seminal fluid protein Sex Peptide (SP), a ligand of the Sex Peptide Receptor (SPR) that is transferred to females during copulation, is responsible for this reduction of siesta sleep. Here, we investigated further the role of SPR in sleep regulation in <I>Drosophila</I>. We show that SPR is required for sleep stabilization in both sexes and that in mutant flies lacking SPR or its ligand myoinhibitory peptide (MIP) sleep is fragmented independently of reproduction. Unlike SP, MIP is expressed in the brain of both sexes and acts on SPR to silence specific neurons that keep flies awake, stabilizing sleep. Hence, our results reveal that SPR interacts with two distinct ligands to control different behaviors: SP for reproduction and MIP for sleep.</P></▼3>
Jeon, Joonho,Kwon, Sang Il,Park, Yong Hee,Oh, Yunjung,Park, Sungwook Elsevier 2014 APPLIED ENERGY Vol.113 No.-
<P><B>Abstract</B></P> <P>The purpose of this study is to investigate the effects of various engine conditions on the combustion, flame temperature and emission characteristics of dimethyl ether (DME) fuel compared with ultra-low sulfur diesel (ULSD) fuel through experimental and numerical analyzes. In order to analyze the temperature distribution, the KIVA-3V code and an optical HSDI diesel engine equipped with a visualization system were employed. The numerical validation was conducted with the experimental results from a DME-fueled compression ignition engine. In addition, measurement of the flame temperature from images captured during the combustion processes was performed using AVL-ThermoVision software.</P> <P>This investigation showed that the combustion pressure and heat release rate attained their peak value at the lowest engine speed condition for DME and ULSD fuels. The characteristics of the flame temperature value and distribution due to the differences in engine speed conditions and fuel properties were clearly revealed. When the engine speed increased, an inhomogeneous and low combustion temperature was observed. Furthermore, the nitrogen oxides (NO<I> <SUB>x</SUB> </I>) emissions, which are related to the combustion temperature, decreased as the engine speed increased.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Experimental and numerical approaches were used for DME combustion. </LI> <LI> DME fuel properties strongly affected temperature distribution. </LI> <LI> The differences of temperature distribution were showed under various conditions. </LI> <LI> DME combustion and emissions characteristics in comparison with ULSD fuel. </LI> </UL> </P>
The Association Between Long Working Hours and Infertility
Ahn Joonho,Lee Sang Ha,Park Min Young,Oh Soo Hyun,Lee Wanhyung 한국산업안전보건공단 산업안전보건연구원 2021 Safety and health at work Vol.12 No.4
Background: This study aimed to investigate whether working long hours was related to infertility among female Korean workers, while taking age into consideration. Methods: We used data from the 2018 National Survey on Fertility and Family Health and Welfare in Korea that is a cross-sectional, nationally representative, and population-based survey. Infertility was defined as women who were not pregnant after regular unprotected intercourse for a year. Working long hours was classified as ≥52 hours, and subgroups as per age were classified on the basis of being younger or older than 40 years of age. Differences in infertility risk between the long working hour group and none were estimated in crude and fully adjusted logistic regression models with age-group stratification. Results: Of 5,909 Korean female workers, the crude and adjusted odds ratios (95% confidence intervals) of infertility for working long hours were 1.295 (0.948-1.737) and 1.303 (0.921-1.809), respectively. In the subgroup of patients below 40 years of age, the crude and adjusted odds ratios (95% confidence interval) were 1.957 (1.216-3.039) and 1.921 (1.144-3.120), whereas those aged 40 years or older had 0.994 (0.647-1.471) and 0.939 (0.560-1.501), respectively. The weighted prevalence of infertility increased as weekly working hours increased only for the younger than 40-year subgroup. Conclusions: Infertility is associated with working long hours, especially in young-aged workers. Thus, the working schedule must be structured to better suit young female workers.