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John, Johnson V.,Chung, Chung-Wook,Johnson, Renjith P.,Jeong, Young-Il,Chung, Kyu-Don,Kang, Dae Hwan,Suh, Hongsuk,Chen, Hongyu,Kim, Il American Chemical Society 2016 Biomacromolecules Vol.17 No.1
<P>Smart delivery system of photosensitizer chlorin e6 (Ce6) has been developed for targeted photodynamic therapy (PDT). Simple self-assemblies of the mixtures comprising soybean lecithin derived phosphatidylcholine (PC), phosphatidylethanolamine-poly(L-histidine)(40) (PE-p-(His)(40)), and folic acid (FA) conjugated phosphatidylethanolamine-poly(N-isopropylacrylamide)(40) (PE-p(NIPAM)(40)-FA) in different ratios yield smart nanospheres characterized by (i) stable and uniform particle size (similar to 100 nm), (ii) positive surface charge, (iii) high hydrophobic drug (Ce6) loading efficiency up to 45%, (iv) covalently linked targeting moiety, (v) low cytotoxicity, and (vi) smartness showing p(His) block oriented pH and p(NIPAM) oriented temperature responsiveness. The Ce6-encapsulated vesicular nanospheres (Ce6@VNS) were used to confirm the efficiency of cellular uptake, intracellular distribution, and phototoxicity against KB tumor cells compared to free Ce6 at different temperature and pH conditions. The Ce6@VNS system showed significant photodynamic therapeutic efficiency on KB cells than free Ce6. A receptor-mediated inhibition study proved the site-specific delivery of Ce6 in targeted tumor cells.</P>
John, Johnson V.,Uthaman, Saji,Augustine, Rimesh,Chen, Hongyu,Park, In-Kyu,Kim, Il Royal Society of Chemistry 2017 Journal of Materials Chemistry B Vol. No.
<P>A series of dual stimuli-responsive poly(l-histidine)n-<I>S-S</I>-polyurethane-<I>S-S</I>-poly(l-histidine)n [p(His)n-<I>SS</I>-PU-<I>SS</I>-p(His)n; <I>n</I> = 25, 35, 50, and 75] triblock copolymers that bear two pH-responsive p(His)n end-blocks and PU middle-blocks tethered by a redox-responsive disulphide linker have been synthesized. The resulting triblock copolymers self-assemble to form micelles, nanodaisies (NDs), of uniform size (∼100 nm) and efficiently encapsulate the anticancer drug doxorubicin (Dox) with a high drug loading content (∼19%). The <I>in vitro</I> release profile shows an enhanced release of Dox in an acidic environment in the presence of 10 mM glutathione. The <I>in vitro</I> cell viability assays performed in various cell lines show that the NDs have no acute or intrinsic toxicity. Confocal microscopy images and flow cytometry results show the pH-responsive cellular uptake of Dox-loaded NDs, accelerated at pH ≤ 5.0. The tumour accumulation and <I>in vivo</I> bio-distribution studies of near-infrared dye (IR-820)-labeled NDs show higher tumour accumulation in CT26 tumour-bearing mice within 72 h. Furthermore, the Dox-loaded NDs effectively inhibit the CT26 tumours, suggesting that they are promising nanocarriers for cancer therapy.</P>
Johnson, Renjith P.,Jeong, Young−,Il,John, Johnson V.,Chung, Chung-Wook,Kang, Dae Hwan,Selvaraj, Manickam,Suh, Hongsuk,Kim, Il American Chemical Society 2013 Biomacromolecules Vol.14 No.5
<P>A series of dual stimuli responsive synthetic polymer bioconjugate chimeric materials, poly(<I>N</I>-isopropylacrylamide)<SUB>55</SUB>-<I>block</I>-poly(<SMALL>l</SMALL>-histidine)<SUB><I>n</I></SUB> [p(NIPAM)<SUB>55</SUB>-<I>b</I>-p(His)<SUB><I>n</I></SUB>] (<I>n</I> = 50, 75, 100, 125), have been synthesized by employing reversible addition–fragmentation chain transfer polymerization of NIPAM, followed by ring–opening polymerization of α-amino acid <I>N</I>-carboxyanhydrides. The dual stimuli responsive properties of the resulting biocompatiable and membrenolytic p(NIPAM)<SUB>55</SUB>-<I>b</I>-p(His)<SUB><I>n</I></SUB> polymers are investigated for their use as a stimuli responsive drug carrier for tumor targeting. Highly uniform self-assembled micelles (∼55 nm) fabricated by p(NIPAM)<SUB>55</SUB>-<I>b</I>-p(His)<SUB><I>n</I></SUB> polymers display sharp thermal and pH responses in aqueous media. An anticancer drug, doxorubicin (Dox), is effectively encapsulated in the micelles and the controlled Dox release is investigated in different temperature and pH conditions. Antitumor effect of the released Dox is also assessed using the HepG2 human hepatocellular carcinoma cell lines. Dox molecules released from the [p(NIPAM)<SUB>55</SUB>-<I>b</I>-p(His)<SUB><I>n</I></SUB>] micelles remain biologically active and have stimuli responsive capability to kill cancer cells. The self-assembling ability of these hybrid materials into uniform micelles and their efficiency to encapsulate Dox makes them a promising drug carrier to cancer cells. The new chimeric materials thus display tunable properties that can make them useful for a molecular switching device and controlled drug delivery applications needing responses to temperature and pH for the improvement of cancer chemotherapy.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bomaf6/2013/bomaf6.2013.14.issue-5/bm400089m/production/images/medium/bm-2013-00089m_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bm400089m'>ACS Electronic Supporting Info</A></P>
V. Vijayakumar,Pradeep Johnson,Ruban Whenish,A. John Rajan,Vincent H Wilson 한양대학교 세라믹연구소 2021 Journal of Ceramic Processing Research Vol.22 No.4
The characteristics of Al 6061/SiCp Metal Matrix Composite (MMC) have been established for checking the suitability oflightweight spur gear using Powder Metallurgy (P/M) technique. Aluminium powder reinforced with SiCp particle withvarious weight.% (0, 5, 10 & 15) and particle sizes (35 μm and 65 μm). The smaller sized spur gears of 4 mm module, 48 mmpitch circle diameter and 22 mm face width were chosen. The complex gear profiled compaction die assembly was manufacturedfor making net shaped gear without any machining operation. The H frame hydraulic press was used to compact gear sampleswith compaction pressures of 132 MPa and 165 MPa. Then gear samples were sintered using muffle furnace at 570 ºC. Theprepared gear samples were used for density measurement, bending strength test, hardness measurement and microstructureanalysis. The research work leads to the observation of Al 6061 with 10 wt.% & 35 μm size of (SiCp) P/M gear exhibiting agreater enhancement of mechanical properties than the unreinforced Al alloy. Thus the effect of adding ceramic particles SiCpwith Al 6061 enhanced the mechanical properties and enabling Al 6061/SiCp composite spur gear suitable for lightweightapplications.
Johnson, Renjith P,Uthaman, Saji,John, Johnson V,Lee, Hye Ri,Lee, Sang Joon,Park, Huiju,Park, In-Kyu,Suh, Hongsuk,Kim, Il American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.39
<P>A series of poly(ethylene glycol) methyl ether acrylate-block-poly(l-lysine)-block-poly(l-histidine) [p(PEGA)30-b-p(Lys)25-b-p(His)n] (n = 25, 50, 75, 100) triblock copolypeptides were designed and synthesized for tumoral pH-responsive intracellular release of anticancer drug doxorubicin hydrochloride (Dox). The tumoral acidic pH-responsive hybrid vesicles fabricated were stable at physiological pH 7.4 and could gradually destabilize in acidic pH as a result of pH-induced swelling of the p(His) block. The blank vesicles were nontoxic over a wide concentration range (0.01-100 관g/mL) in normal cell lines. The tumor acidic pH responsiveness of these vesicles was exploited for intracellular delivery of Dox. Vesicles efficiently encapsulated Dox, and pH-induced destabilization resulted in the controlled and sustained release of Dox in CT26 murine cancer cells, and dose-dependent cytotoxicity. The tumor-specific controlled release Dox from vesicles demonstrates this system represents a promising theranostic agent for tumor-targeted delivery.</P>