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Jinlong Zhang,Jingkun Lu,Bing Liu,Qiuyue Liu,Fan Jin,Miaojun Zhang,Yerong Liu,Yujun Song,Chenhui Dong,Wanyi Zhang,Ningxu Han,Xu Deng,Feng Xing 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.S1
Quantification of viable spores is a time taking task due to the lack of rapid, efficient and accurate methods. This studypresented a simple spectrophotometric method for the detection of viable spores based on spore’s property of losing refractivityduring the germination process. By comparison of the results obtained by both spectrophotometric method and colonycounting method, a good linear correlation (R2 = 0.99) was achieved between viable spore concentration and OD loss underappropriate conditions. To avoid interference from ungerminable spores and vegetative cells, a turbidity complementationstrategy of keeping the initial concentration of spore suspensions at the same and relatively lower level was required. Thecalibration equation developed could be used to predict the viable spore yield produced in a series of fermentation experiments. The experimental results proved that this novel spectrophotometric method was sensitive, rapid, and easy to performcompared to conventional colony counting method.
Improving the Electrical Conductivity of PEDOT:PSS Films by Binary Secondary Doping
Zhengyou Zhu,Congcong Liu,Jingkun Xu,Qinglin Jiang,Hui Shi,Endou Liu 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.1
In this work, the electrical conductivity of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) films was effectively enhanced by binarysecondary doping. Initially, doping with 5 vol.% dimethyl sulfoxide (DMSO)improved the electrical conductivity from 0.3 S cm−1 to 437 S cm−1 and a furtherincrease to 950 S cm−1 was achieved by adding LiClO4. The conductivity value wereport here is one of the highest reported for pretreated PEDOT:PSS films. Theobtained maximum electrical conductivity is almost 3000 times higher than thatshown by pristine PEDOT:PSS films. The increase in the electrical conductivity isascribed to the synergistic effect of the two dopants. Fourier transform infraredspectra indicated the absence of any changes to the chemical structure ofPEDOT:PSS. Atomic force microscopy images demonstrate an increased surfaceroughness and suggest the occurrence of conformational changes of PEDOT chainsfrom the coiled to coil-extended one, which is the key reason for the electricalconductivity enhancement. The pretreatments we propose here are rapid, simple andeffective for the large-scale preparation of high-conductivity PEDOT:PSS films.
Hongfei Zhu,Congcong Liu,Haijun Song,Jingkun Xu,Fangfang Kong,Jianmin Wang 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.2
Poly(3-hexylthiophene) (P3HT) films doped with iodine vapor have been prepared by casting a P3HT solution on glass substrates and their thermoelectric (TE) performances has been investigated. The maximum Seebeck coefficient and electrical conductivity of iodine-doped P3HT films were 386 μV·K−1 (at room temperature) and 4.7 × 10−1 S·cm−1, which is about five orders of magnitude higher than that of pristine P3HT films. The power factor of these iodine-doped P3HT films was estimated to be 7.0 μW·m−1·K−2 at room temperature, which is a relative high value for organic TE materials. The UV-vis spectra of iodine-doped P3HT films showed a slight red shift of the iodine-doped P3HT compared to those of pristine P3HT films. Atomic force microscopy images indicated the conformational changes in P3HT chains after treatment with iodine vapor. During this treatment, the P3HT chains self-organized into a more ordered structure, this organization improved the charge carrier transport capability and the TE performance of P3HT the films.
New dammarane-type triterpenoid saponins from Panax notoginseng saponins
Qian Li,Mingrui Yuan,Xiaohui Li,Jinyu Li,Ming Xu,Di Wei,Desong Wu,Jinfu Wan,Shuangxi Mei,Tao Cui,Jingkun Wang,Zhaoyun Zhu 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.5
Background: Panax notoginseng saponin (PNS) is the extraction from the roots and rhizomes of Panax notoginseng (Burk.) F. H. Chen. PNS is the main bioactive component of Xuesaitong, Xueshuantong, and other Chinese patent medicines, which are all bestselling prescriptions in China to treat cardiocerebrovascular diseases. Notoginsenoside R₁ and ginsenoside Rg₁, Rd, Re, and Rb₁ are the principal effective constituents of PNS, but a systematic research on the rare saponin compositions has not been conducted. Objective: The objective of this study was to conduct a systematic chemical study on PNS and establish the HPLC fingerprint of PNS to provide scientific evidence in quality control. In addition, the cytotoxicity of the new compounds was tested. Methods: Pure saponins from PNS were isolated by means of many chromatographic methods, and their structures were determined by extensive analyses of NMR and HR-ESI-MS studies. The fingerprint was established by HPLC-UV method. The cytotoxicity of the compounds was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5 -diphenyltetrazolium bromide assay. Results and Conclusion: Three new triterpenoid saponins (1e3) together with 25 known rare saponins (4 e28) were isolated from PNS, except for the five main compounds (notoginsenoside R₁ and ginsenoside Rg₁, Rd, Re, and Rb₁). In addition, the HPLC fingerprint of PNS was established, and the peaks of the isolated compounds were marked. The study of chemical constituents and fingerprint was useful for the quality control of PNS. The study on antitumor activities showed that new Compound 2 exhibited significant inhibitory activity against the tested cell lines.