Physiological and psychological responses while wearing firefighters’ protective clothing under various ambient conditions

He, Jiazhen,Park, Eunjin,Li, Jun,Kim, Eunae SAGE Publications 2017 Textile Research Journal Vol.87 No.8

ANXA3, associated with YAP1 regulation, participates in the proliferation and chemoresistance of cervical cancer cells

Huang Jiazhen,Wei Wei,Kang Fuli,Tan Shuang,Li Yibing,Lu Xiaohang,Wang Ning 한국유전학회 2023 Genes & Genomics Vol.45 No.12

Background Cervical cancer, as one of the most common cancers in women, remains a major health threat worldwide. Annexin A3 (ANXA3), a component of the annexin family, is upregulated in numerous cancers, with no explicit role in cervical cancer. Objective This study aims to investigate the function of ANXA3 in cervical cancer. Methods Differential expression genes between the cervical cancer tissues of patients and the controls were analyzed in The Cancer Genome Atlas (TCGA) and Gene Expression Profiling Interactive Analysis (GEPIA) database. Using transfection approaches to either upregulate or downregulate ANXA3, its role in cell proliferation and chemosensitivity of human cervical cancer cell lines (HeLa and C33A) was evaluated. Furthermore, the binding activity between YAP1 and ANXA3 was also explored. Results Genomics analysis indicated that differential genes were mostly associated with cell cycle progression and DNA replication. ANXA3 was highly expressed in the cervical cancer tissues and closely linked to malignancy degree. Knockdown of ANXA3 in cervical cancer cells inhibited cell cycle progression. A similar result was observed in the reduction of cyclin D, CDK4, cyclin E, and CDK2 in cervical cancer cells with ANXA3 silencing. Cervical cancer cells obtained high sensitivity to cisplatin (DDP) when ANXA3 was downregulated. Conversely, these capabilities were the opposite in cervical cancer cells overexpressing ANXA3. Furthermore, the expression levels of ANXA3 and YAP1 were positively correlated. YAP1 upregulation was positively connected with malignant behaviors, which were reversed by ANXA3 downregulation. Conclusion In light of our findings, targeting ANXA3 expressed in cervical cancer might contribute to more potential therapeutic strategies.

A transcriptional atlas of the silk gland in Antheraea pernyi revealed by IsoSeq

Duan Jianping,Li Shanshan,Zhang Zhengtian,Yao Lunguang,Yang Xinfeng,Ma Sanyuan,Duan Nini,Wang Jiazhen,Zhu Xuwei,Zhao Ping 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Silk fibers spun by the silk gland of Antheraea pernyi have many unique properties and are of great value in genetic improvement and non-traditional applications. However, the complete transcriptional landscape and accurate genic annotation of the silk gland are yet to be conducted, which limits related studies on this organ. In this study, isoform sequencing revealed the full-length transcriptome of the A. pernyi silk gland, producing 12,572 high-confidence isoforms from 7,658 genes, among which more than 40 % of genes have not yet been annotated in the reference genome. Moreover, approximately 9 % of isoforms are computationally identified as long non-coding RNAs (lncRNAs). Up to 1,492 alternative splicing (AS) and 3,068 alternative polyadenylation (APA) events were revealed within this transcriptome. In addition, 2,569 putative transcription factors (TFs) belonging to 68 different families were first identified in A. pernyi genome, including 871 TFs in silk gland, and some TF families have undergone expansion or contraction. This study significantly improve our knowledge of the genes expressed in the silk gland of A. pernyi and provide a valuable resource for the in-depth study of silk protein synthesis and spinning, genetic improvement, and non-traditional applications in A. pernyi.

BAIAP2L2 facilitates the malignancy of prostate cancer (PCa) via VEGF and apoptosis signaling pathways

Yuanzi Song,Guishan Zhuang,Jiazhen Li,Mingqing Zhang 한국유전학회 2021 Genes & Genomics Vol.43 No.4

Background Prostate cancer (PCa) is the second most common type of male cancer in western. Despite key roles of brainspecifc angiogenesis inhibitor 1-associated protein like 2 (BAIAP2L2) in several cancers, the function of BAIAP2L2 in PCa is never reported. Objective We aimed to investigate the role of BAIAP2L2 in the progression of PCa and decipher the underlying mechanisms. Methods RNA sequencing data from TCGA database were used to evaluate the expression of BAIAP2L2 in PCa. Survival analysis and Cox regression model analysis were conducted to evaluate the prognostic value of BAIAP2L2. BAIAP2L2- associated pathways were preliminary analyzed by Gene Set Enrichment Analysis (GSEA) method and confrmed by western blot assays. Cell proliferation and transwell assays were performed to determine biological behaviors in BAIAP2L2 knockeddown or overexpressed PCa cell lines including LNCaP and PC-3 cells. Results In our study, BAIAP2L2 was signifcantly up-regulated in PCa tissues and cell lines and independently associated with the poor prognosis of PCa patients. Knockdown of BAIAP2L2 notably repressed proliferation, migration and invasion of PCa cells. And overexpression of BAIAP2L2 obtained the contrary results. Mechanically, GSEA method and western blot results of key molecules in signaling pathways implicated that the depletion of BAIAP2L2 inactivated the vascular endothelial growth factors (VEGFs) and induced apoptosis signaling pathways in PCa cells. Conclusions Overall, these fndings revealed that BAIAP2L2 may support tumorigenesis and malignant development of prostate cancer cells via VEGF and apoptosis signaling pathways, and it could be considered as a promising biomarker and independent prognostic predictor of prostate cancer.

Tissue-associated profiling of gene expression in the fifth-instar larvae of Chinese Oak Silkworm, Antheraea pernyi

Duan Jianping,Liang Shimei,Zhu Zhenni,Yang Xinfeng,Li Ying,Xu Xin,Wang Jiazhen,Zhu Xuwei,Yao Lunguang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Antheraea pernyi is an edible insect with important economic value in both traditional and non-traditional ap plications. Elucidating the details of gene expression variation among different A. pernyi larval tissues will greatly improve our knowledge about A. pernyi biology and promote its protection and breeding. Here, tissue-based transcriptome and gene co-expression network analysis were performed for the fifth-instar larval tissues of testis, midgut, Malpighian tubule, head, trachea, silk gland, integument, hemocyte, ovary, and fat body. A total of 1,991 tissue-associated genes, including 62 transcription factor-encoding genes, were correlated with the development and functions of these tissues. Moreover, 10 gene co-expression modules that were highly corre lated with the tissues were also determined. The most important 30 genes with top connectivity in each module were identified as hub genes to form the core co-expression network with important biological functions in the corresponding tissues. Based on these resources, further in-depth studies should be conducted to determine the roles of candidate genes and transcription factors governing tissue development and trait formation in A. pernyi.

Morphine-6-Glucuronide Isomers-Synthesis and Biological Evaluation

Jixia Yang,Guanyi Lu,Gongzheng Zhang,Xiaodi Wang,Hongliang Wen,Cipan Huang,Jiazhen Yin,Jin Li 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.11

Morphine-6?-D-glucuronide (M6?G), an active metabolite of morphine, and its isomer morphine-6α-D-glucuronide (M6αG) were synthesized from 3-O-protected morphinethrough glycosylation and alkaline hydrolysis. All structures were determined by spectroscopic analysis, and especially it is the first time to report the single crystal of compound M6αG. In vitro binding assay showed that M6?G bound to mu opioid receptor (MOR), kappa opioid receptor (KOR), and delta opioid receptor (DOR) with nanomolar affinity (Ki = 28.03, 116.88, and 375.13?nM) and M6αG bound to them with similar affinity (Ki = 1070.13, 20?637.93, and 677.36?nM). The selectivity of M6αG toward KOR is much higher. Hot-plate test showed that the analgesic effect of M6?G is better than that of M6αG, that is maybe because the mechanism of M6αG is different.