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Ick Hyun Jo,Young Chang Kim,Dong Hwi Kim,Kee Hong Kim,Tae Kyung Hyun,Hojin Ryu,Kyong Hwan Bang 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.4
The development of molecular markers is one of the most useful methods for molecular breeding and marker-based molecular associated selections. Even though there is less information on the reference genome, molecular markers are indispensable tools for determination of genetic variation and identification of species with high levels of accuracy and reproducibility. The demand for molecular approaches for marker-based breeding and genetic discriminations in Panax species has greatly increased in recent times and has been successfully applied for various purposes. However, owing to the existence of diverse molecular techniques and differences in their principles and applications, there should be careful consideration while selecting appropriate marker types. In this review, we outline the recent status of different molecular marker applications in ginseng research and industrial fields. In addition, we discuss the basic principles, requirements, and advantages and disadvantages of the most widely used molecular markers, including restriction fragment length polymorphism, random amplified polymorphic DNA, sequence tag sites, simple sequence repeats, and single nucleotide polymorphisms.
인삼 염류저항성 계통 G07006의 엽록체 유전자 해독 및 변이분석
Ick Hyun Jo,Chi Eun Hong,Young Chang Kim,Jang Uk Kim,Jung Woo Lee,Dong Hwi Kim,Dong Yun Hyun 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : The P. ginseng breeding line G07006, was selected for salt tolerance through salinity screening of mature leaves at the NIHHS of the RDA in 2014-2016. However, it is difficult to maintain a genetically stable breeding line of cross-pollinating crop in the field. Therefore molecular marker required to identify and maintain breeding line G07006. Methods and Results : DNA was extracted following the CTAB DNA extraction protocol (Doyle and Doyle, 1987) with modifications. A pair-end (PE) library was constructed and sequenced using an Illumina MiSeq platform by Lab Genomics, Inc. (Seongnam, Korea). Approximately 4.0 Gb of sequencing data were obtained, and de novo assembled by a CLC genome assembler(v. beta 4.6, CLC Inc., Rarhus, Denmark). The complete chloroplast(CP) genome size is 156,356 bp, including two inverted repeats (IRs) of 52,060 bp, separated by the large single-copy (LSC 86,174 bp) and small single-copy (SSC 18,122 bp) regions. This CP genome encodes 114 unigenes (80 protein-coding genes, four rRNA genes, and 30 tRNA genes), in which 18 are duplicated in the IR regions. Conclusion : This complete chloroplast DNA sequence will provide conducive to discriminate line G070006 (salt-tolerant) and further enhancing genetic improvement program of this important medical plant.
Rapid Identification of Ginseng Cultivars (Panax ginseng Meyer) Using Novel SNP-Based Probes
Ick-Hyun Jo,Kyong Hwan Bang,Young-Chang Kim,Jei-Wan Lee,A-Yeon Seo,Bong-Jae Seong,Hyun-Ho Kim,Dong-Hwi Kim,Seon-Woo Cha,Yong-Gu Cho,Hong-Sig Kim 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.4
In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP II 0, and PGP 130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as "ATA", "GCC", "GTA", "GCA", and" ACC", respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.
Evaluating Cold Resistance of Ginseng Germplasm using Chlorophyll Fluorescence
Ick Hyun Jo(조익현),Woo Jong Jang(장우종),Jung Woo Lee(이정우),Sung Min Jung(정성민),Na Young Kwon(권나영),Young Chang Kim(김영창),Chi Eun Hong(홍지은),Kyong Hwan Bang(방경환),Jang Uk Kim(김장욱),Dong Hwi Kim(김동휘) 한국약용작물학회 2022 한국약용작물학회 학술대회논문집 Vol.2022 No.1
Rapid Identification of Ginseng Cultivars (Panax ginseng Meyer) Using Novel SNP-Based Probes
Jo, Ick-Hyun,Bang, Kyong-Hwan,Kim, Young-Chang,Lee, Jei-Wan,Seo, A-Yeon,Seong, Bong-Jae,Kim, Hyun-Ho,Kim, Dong-Hwi,Cha, Seon-Woo,Cho, Yong-Gu,Kim, Hong-Sig The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.4
In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P. ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP110, and PGP130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as 'ATA', 'GCC', 'GTA', 'GCA', and 'ACC', respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.
Jo, Ick-Hyun,Bang, Kyong-Hwan,Hong, Chi Eun,Kim, Jang-Uk,Lee, Jung-Woo,Kim, Dong-Hwi,Hyun, Dong-Yun,Ryu, Hojin,Kim, Young-Chang The Korean Society of Plant Biotechnology 2016 식물생명공학회지 Vol.43 No.4
The complete chloroplast genome sequence of Panax ginseng breeding line 'G07006', showing higher salt tolerance, was confirmed by de novo assembly using whole genome next-generation sequences. The complete chloroplast (CP) genome size is 156,356 bp, including two inverted repeats (IRs) of 52,060 bp, separated by the large single-copy (LSC 86,174 bp) and the small single-copy (SSC 18,122 bp) regions. One hundred fourteen genes were annotated, including 80 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Among them, 18 sites were duplicated in the inverted repeat regions. By comparative analyses of the previously identified CP genome sequences of nine cultivars of P. ginseng and that of G07006, five useful SNPs were defined in this study. Since three of the five SNPs were cultivar-specific to Chunpoong and Sunhyang, they could be easily used for distinguishing from other ginseng accessions. However, on arranging SNPs according to their gene location, the G07006 genotype was 'GTGGA', which was distinct from other accessions. This complete chloroplast DNA sequence could be conducive to discrimination of the line G07006 (salt-tolerant) and further enhancement of the genetic improvement program for this important medicinal plant.
염류처리에 따른 인삼 세포막 수분채널 유전자의 발현양상 분석
Ick Hyun Jo,Young Chang Kim,Jang Uk Kim,Jung Woo Lee,Ji Young Lim,Dong Hwi Kim,Kee Hong Kim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.05
Background : Water uptake and flow across cellular membranes is a fundamental requirement for plant growth and development, and plant water status is important not only for plant growth under favorable conditions but also for ability of a plant to tolerate adverse environmental conditions. Thus identification of plasma membrane water channel genes (aquaporins) in ginseng provides extensive information for functional studies and the development of markers for salinity stress tolerance. Methods and Results : For salinity treatment, the plants were grown for 4 weeks in culture medium gelled with 0.8% Phytoagar, and the old media were replaced with the fresh medium containing NaCl at 0, 50, 100, 200 and 400 mM, respectively. The samples for stress treated and non-stressed plants were collected from 6h to 72h, and frozen immediately into liquid nitrogen. According to the sequence information from the assembled transcripts, four primer pairs were designed from the aquaporin gene regions. In order to determine the pattern of aquaporins expression in ginseng seedlings to salinity stress, we conducted semi-quantitative RT-PCR. Conclusion : A tonoplast intrinsic protein 1 (TIP1)-type aquaporin is not only believed to be essential for plant life, but also to be beneficial for growth under salinity stress. Therefore, a deeper understanding of aquaporin genes in ginseng will be essential for crop improvement, which could help us to understand the molecular genetic basis for the ginseng genetic improvement and also provide the functional genetic resources for selective breeding and transgenic research.