Phenol 분해균주의 분리 및 페놀함유 폐수의 생물학적 처리

이현돈(Hyun Don Lee),이명은(Myoung Eun Lee),김형갑(Hyung Gab Kim),서현효(Hyun-Hyo Suh) 한국생명과학회 2013 생명과학회지 Vol.23 No.10

방향족화합물들로 오염되어있는 토양 및 산업폐수를 포함한 각종 시료로부터 phenol에 분해활성이 높은 56균주를 순수분리 하였으며, 이들 분리 균주 중 균체생육과 phenol 분해활성이 가장 높은 균주인 GN13을 선별하였다. 분리균주 GN13은 형태학적, 생리학적 및 생화학적 특성을 조사한 결과 Neisseria 속 세균과 유사한 것으로 판명되어 최종적으로 Neisseria sp. GN13으로 명명하였다. 분리균주 Neisseria sp. GN13의 균체생육 및 phenol 분해를 위한 최적온도와 최적 pH는 각각 32℃와 7.0였다. 유일 탄소원으로 phenol 1,000 mg/l를 포함하여 최적화된 배지를 사용한 jar-fermentor 배지에서 배양 30시간에 균체생육이 최대에 이르렀으며 배양 27시간째 거의 모든 phenol이 분해되었으며, catechol deoxygenase 활성측정에 의하여 Neisseria sp. GN13은 meta-와 ortho-pathway를 통하여 catechol 분해가 일어났다. Neisseria sp. GN13은 phenol 함유 인공폐수에서의 phenol 분해율은 배양 30시간 만에 97%의 phenol이 분해되는 것으로 나타났으며, 인공폐수에 대한 Neisseria sp. GN13과 활성슬러지 처리구에서의 TOC 제거효율은 각각 83%와 78%였다. 석유화학폐수에 대한 Neisseria sp. GN13의 COD 제거율은 활성슬러지만을 포함한 대조구보다 약 1.3배 높은 효율을 나타내었다. 이러한 결과로 미루어 분리균주 Neisseria sp. GN13은 phenol을 함유하고 있는 여러 폐수에 효과적으로 적용될 수 있을 것으로 생각된다. Aromatic hydrocarbons, such as phenol, have been detected frequently in wastewater, soil, and groundwater because of the extensive use of oil products. Bacterial strains (56 isolates) that degraded phenol were isolated from soil and industrial wastewater contaminated with hydrocarbons. GN13, which showed the best cell growth and phenol degradation, was selected for further analysis. The GN13 isolate was identified as Neisseria sp. based on the results of morphological, physiological, and biochemical taxonomic analyses and designated as Neisseria sp. GN13. The optimum temperature and pH for phenol removal of Neisseria sp. GN13 was 32°C and 7.0, respectively. The highest cell growth occurred after cultivation for 30 hours in a jar fermentor using optimized medium containing 1,000 mg/l of phenol as the sole carbon source. Phenol was not detected after 27 hours of cultivation. Based on the analysis of catechol dioxygenase, it seemed that catechol was degraded through the meta- and ortho-cleavage pathway. Analysis of the biodegradation of phenol by Neisseria sp. GN13 in artificial wastewater containing phenol showed that the removal rate of phenol was 97% during incubation of 30 hours. The removal rate of total organic carbon (TOC) by Neisseria sp. GN13 and activated sludge was 83% and 78%, respectively. The COD removal rate by Neisseria sp. GN13 from petrochemical wastewater was about 1.3 times higher than that of a control containing only activated sludge.

Flocculating Properties of Bioflocculant Biopol32 from Pseudomonas sp. GP32

Hyun Don Lee(이현돈),Oh Nara(오나라),Muyeong Eun Lee(이명은),Hyun Hyo Suh(서현효) 한국생명과학회 2017 생명과학회지 Vol.27 No.8

Pseudomonas sp. GP32가 생산하는 생물고분자응집제 Biopol32의 실제 산업폐수에서의 적용을 위하여 생물고분자 Biopol32의 응집특성을 조사하였다. Biopol32의 응집물질은 polysaccharide로 확인되었다. 음이온성 응집제들은 응집효율을 높이기 위하여 보조응집제로 counter ion을 사용하고 있다. Biopol32의 응집활성은 보조응집제로 Ca<SUP>2+</SUP>, Al<SUP>3+</SUP>와 같은 양이온을 첨가 하였을 때 크게 증가하였으며, 보조응집제로서 7.0 mM CaCl₂ · 2H₂O을 첨가하였을 때 Biopol32의 응집활성이 가장 높게 나타났다. Kaolin 현탁액에 Biopol32를 1.5 mg/l의 농도로 첨가하였을 때 가장 높은 응집활성을 보였다. Biopol32의 pH와 온도에 따른 응집활성은 현재 폐수처리 현장에서 상업적으로 이용되고 있는 음이온성 유기합성고분자응집제 polyacrylamide와 Zoogloea ramigera로부터 생산된 생물고분자응집제 zooglan과 응집활성을 비교하였다. Biopol32의 응집활성은 pH 5.0에서 8.0의 넓은 범위의 pH에서 높은 응집활성을 보였으며, 또한 온도의 영향에서는 60℃에서 가장 높은 응집활성을 나타내었으나, 70℃ 이상의 온도에서는 응집활성이 급격히 감소하는 것으로 나타났다. Jar fermentor를 이용한 batch culture를 통하여 Biopol32의 응집활성과 전하밀도와의 관계를 조사하였다. 음이온성 전하밀도와 겉보기 점도가 높을수록 Biopol32의 응집활성이 높아져, Biopol32의 응집활성과 겉보기점도는 Biopol32의 전하밀도와 밀접한 관계가 있는 것으로 나타났다. The flocculating properties of bioflocculant Biopol32 produced by Pseudomonas sp. GP32 were investigated for application in industrial wastewater treatment. The major flocculating substance of bioflocculant Biopol32 was identified as polysaccharide. Many anionic flocculants need a counter ion to induce higher flocculating activity. The flocculating activity of bioflocculant Biopol32 was markedly increased by the addition of cationic ions (Ca<SUP>2+</SUP>, Al<SUP>3+</SUP>). The flocculating activity of bioflocculant Biopol32 was the most effective when 7.0 mM CaCl₂ · 2H₂O as coflocculant was added. The flocculating activity on the effect of pH and the temperature of the bioflocculant Biopol32 was compared with anionic commercial flocculant (polyacrylamide) and bioflocculant (zooglan from Zoogloea ramigera). In kaolin suspension, the highest flocculating activity was obtained at the bioflocculant Biopol32 concentration of 1.5 mg/l. A high flocculating activity was observed in the pH range of 5.0 to 8.0. The flocculating activity of bioflocculant Biopol32 was sustained up to 60℃, but decreased rapidly at over 70℃. In the batch culture, the charge density of bioflocculant Biopol32 was compared with flocculating activity. The larger the anionic charge density and apparent viscosity of bioflocculant Biopol32, the higher the flocculating activity. Therefore, we confirmed that the flocculating activity and apparent viscosity of bioflocculant Biopol32 was closely related to the charge density of bioflocculant Biopol32.

펄스 코로나 방전에 의한 액체상 페놀 전환 특성

이현돈(Hyun Don Lee),정재우(Jae Woo Chung),조무현(Moo Hyun Cho) 大韓環境工學會 2007 대한환경공학회지 Vol.29 No.1

유기염소계 난분해성 산업폐수의 처리를 위한 미생물제제의 개발

이현돈(Hyun Don Lee),임성원(Seong Won Im),서현효(Hyun-Hyo Suh) 한국생명과학회 2014 생명과학회지 Vol.24 No.8

유기염소계 난분해성 산업폐수처리에 효과적인 미생물제제 개발을 위하여 PCP (pentachlorophenol)와 TCE (trichloroethylene) 등과 같은 유기계 염소화합물이 오염되어있는 토양 및 산업폐수로부터 PCP 분해활성이 높은 GP5, GP19와 TCE 분해활성이 높은 GA6, GA15를 분리하였다. 이들 분리균주 GP5, GP19, GA6, GA15등은 Acetobactor sp., Pseudomonas sp., Arthrobacer sp., Xanthomonas sp.과 유사한 것으로 나타나 최종적으로 Acetobacter sp. GP5, Pseudomonas sp. GP19, Arthrobacer sp. GA6, Xanthomoas sp. GA15로 명명하였다. 유기염소계 산업폐수의 처리를 위한 복합미생물제제 OC17은 PCP와 TCE를 분해하는 4개의 분리 분리균주와 방향족화합물 분해균주인 Acinetobacter sp. KN11, Neisseria sp. GN13의 배양액을 혼합하여 제조하였다. 복합미생물제제 OC17은 2.8×10<SUP>9</SUP> CFU/g의 균체수를 갖고 있으며, 밀도는 0.299 g/㎤, 수분함량은 26.8%를 나타내었다. 복합미생물 제제 OC17은 PCP 500 mg/l가 포함되어있는 인공폐수를 이용한 실험에서 배양 65시간에 87%의 분해효율을 나타내었고, TCE (300 uM)의 분해효율은 배양 50시간에 90%의 분해효율을 나타내었다. 복합미생물제제 OC17을 이용한 유기 염소계 산업폐수의 처리효율 시험을 위한 연속배양 실험 에서 10일간 처리 하였을 때 91%의 COD 제거효율을 나타내었다. The screening of the microorganisms degrading chlorinated organic compounds such as PCP (pentachlorophenol) and TCE (trichloroethylene) was conducted with soil and industrial wastewater contaminated with various chlorinated organic compounds. Isolates (GP5, GP19) capable of degrading PCP and isolates (GA6, GA15) capable of degrading TCE were identified as Acetobactor sp., Pseudomonas sp., Arthrobacer sp., Xanthomonas sp. and named Acetobacter sp. GP5, Pseudomonas sp. GP19, Arthrobacer sp. GA6 and Xanthomoas sp. GA15, respectively. The microbial augmentation, OC17 formulated with the mixture of bacteria including isolates (4 strains) degrading chlorinated organic compounds and isolates (Acinetobacter sp. KN11, Neisseria sp. GN13) degrading aromatic hydrocarbons. Characteristics of microbial augmentation OC-17 showed cell mass of 2.8×10<SUP>9</SUP> CFU/g, bulk density of 0.299 g/㎤ and water content of 26.8%. In the experiment with an artificial wastewater containing PCP (500 mg/l), degradation efficiency of the microbial augmentation OC17 was 87% during incubation of 65 hours. The degradation efficiency of TCE (300 uM) by microbial augmentation OC17 was 90% during incubation of 50 hours. In a continuous culture experiment, analysis of the biodegradation of organic compounds by microbial augmentation OC17 in industry wastewater containing chlorinated hydrocarbons showed that the removal rate of COD was 91% during incubation of 10 days. These results indicate that it is possible to apply the microbial augmentation OC17 to industrial wastewaters containing chlorinated organic compounds.

Production and Characterization of Extracellular Polysaccharide Produced by Pseudomonas sp. GP32

Myoung Eun Lee(이명은),Hyun Don Lee(이현돈),Hyun-Hyo Suh(서현효) 한국생명과학회 2015 생명과학회지 Vol.25 No.9

미생물유래 다당류 생산균주를 분리하기 위해 전국 각지의 토양시료로부터 가장 높은 점성과 다당류 생산성을 나타내는 균주 GP32를 분리하였으며, 분리균주 GP32의 동정을 분리균주의 형태학적, 생리학적 특성을 조사한 결과, Pseudomonas 속 세균으로 확인되었으며 최종적으로 Pseudomonas sp. GP32로 명명하였다. 플라스크 수준에서 Pseudomonas sp. GP32의 다당류 생산을 위한 가장 적합한 탄소원과 질소원은galactose와 (NH4)2SO4를 이용하였을 때 가장 많은 다당류를 생산하는 것으로 확인되었으며, 다당류 생산을 위한 최적의 C/N ratio는 50이었다. 다당류 생산을 위한 최적 pH와 온도는 각각 7.5와 32℃였다. 최적화된 배지를 이용한 fermentor 배양에서 다당류 생산은 배양 70시간에 최고치를 나타내었으며, 이때 다당류 생산량은 15.7 g/l이었다. Pseudomonas sp. GP32로부터 생산된 다당류는 ethanol 침전, cetylpyridimium 침전과 gel permeation chromatography를 통하여 정제하였으며, 정제된 다당류는 Biopol32로 명명하였다. Biopol 32의 분자량은 3×107 datons이었으며, Biopol32가 함유하고 있는 구성당은 galactose : glucose : gulcouronic acid : galactouronic acid 등이 1.85 : 3.24 : 1.00 : 1.42의 몰비로 함유되어있다. Biopol32 용액은 의가소성 성질을 갖는 고분자 화합물로서 Zoogloea ramigera가 생산하는 생물고분자인 zooglan보다 모든 농도에서 높은 점성을 나타내었다. Biopol32의 실제 폐수처리현장에서 응집제로의 사용 가능성을 검토하기 위하여 식품폐수, 섬유폐수와 제지폐수를 대상으로 응집효율을 조사한 결과, 높은 COD 감소율(58.4~67.3%)과 SS제거율(82.6~91.3%)를 나타내어 실제 산업폐수에서 뛰어난 응집효율을 나타내었다. A strain GP32 which produces a highly viscous extracellular polysaccharide was conducted with soil samples and identified as Pseudomonas species. The culture flask conditions for the production of extracellular polysaccharide by Pseudomonas sp. GP32 were investigated. The most suitable carbon and nitrogen source for extracellular polysaccharide production were galactose and (NH4)2SO4. The optimum carbon/nitrogen ratio for the production of extracellular polysaccharide was around 50. The optimum pH and temperature for extracellular polysaccharide production was 7.5 and 32℃, respectively. In batch fermentation using a jar fermentor, the highest extracellular polysaccharide content (15.7 g/l) was obtained after 70 hr of cultivation. The extracellular polysaccharide produced by Pseudomonas sp. GP32 (designated Biopol32) was purified by ethanol precipitation, cetylpyridinium chloride (CPC) precipitation, and gel permeation chromatography. Biopol32, which has an estimated molecular weight of over 3×107 datons, is a novel polysaccharide derived from sugar components consisting of galactose, glucose, gulcouronic acid and galactouronic acid in an approximate molar ratio of 1.85 : 3.24 : 1.00 : 1.42. The solution of Biopol32 showed non-Newtonian characteristics. The viscosity of Biopol32 exhibited appeared to be higher at all concentration compared to that of zooglan from Zoogloea ramigera. An analysis of the flocculating efficiency of Biopol32 in industry wastewater (food, textile, and paper wastewater) revealed chemical oxygen demand (COD) reduction rates 58.4-67.3% and suspended solid (SS) removal rates 82.6-91.3%. Based on these results, Biopol32 is a possible candidate for industrial applications such as wastewater treatment.

화력발전소 배출먼지의 전기 집진 특성에 관한 파일럿 규모 실험

정재우(Jae Woo Chung),고경보(Kyung Bo Ko),이현돈(Hyun Don Lee),이용환(Yong Hwan Lee),김용진(Yong Jin Kim),홍원석(Won Seok Hong),박경렬(Kyung Lyoul Park),조무현(Moo Hyun Cho) 大韓環境工學會 2004 대한환경공학회지 Vol.26 No.11

이중 계층 패렐랙스 배리어 기반의 무안경식 3D 디스플레이

이현(Hyun Lee),이응돈(Eung-Don Lee),엄기문(Gi-Mun Um),정원식(Won-Sik Cheong),이성중(Sung Jung Lee) 大韓電子工學會 2011 電子工學會論文誌-SP (Signal processing) Vol.48 No.6

본 논문에서는 기존 무안경식 3D 디스플레이의 제한된 시야각으로 인한 3D 시청에서의 불편함을 해소하기 위해 새로운 패렐랙스 배리어 방식을 제안한다. 제안된 방식은 기존 패렐랙스 배리어의 TN-LCD 구조에서 LC를 공유하는 이중 계층의 ITO쌍을 추가하였으며 시선 추적 장치에 의해 시청자의 위치를 감지하여 자동으로 3D 시야각을 시청자의 위치에 맞도록 변화 시킬 수 있는 이중 계층의 패렐랙스 구조를 갖는다. 제안한 이중 계층 패렐랙스 배리어 방식을 시제품으로 구현하고 실험한 결과 제안한 방식의 유효성을 확인할 수 있었다. We introduce a parallax barrier type autostereoscopic display in order to overcome the limited viewing angle of the conventional parallax barrier type displays. The proposed method adopts a special structure of double pairs of ITO that have a common TN-LC at the core of the conventional parallax barrier. Compared with the conventional parallax barrier type displays the proposed display uses moving parallax barriers and eye tracking system to make stereoscopic view images adapted to the movement of viewer. We implemented a prototype of the proposed dual layer parallax barrier system and verified that the proposed autostereoscopic display maintains seamless 3D views even when a viewer's head is moving.

시분할 방식의 무안경 다시점 3D 디스플레이의 시역 분석

김양수(Yang-Su KIM),이현(Hyun LEE),이응돈(Eung-Don LEE),이광순(Gwangsoon LEE) 한국통신학회 2016 한국통신학회 학술대회논문집 Vol.2016 No.1

超高壓線路의 靜電誘導電壓 低減에 관한 硏究

李鉉敦,韓萬春 연세대학교 산업기술연구소 1982 논문집 Vol.14 No.1

This paper presents the effective method for the reduction of electrostatic induced voltages using shielding wires. Overhead Extra High Voltage(EHV) transmission lines generate an electrostatic field, which imposes additional design consideration in order to avoid excessive induced voltages and the other undesirable effects. A 345 KV EHV transmission line was selected as a model system in order to compute electrostatic induced voltages using electronic computers. The electrostatic induced voltages, after setting up shielding wires,showed more considerable reduction than before.

미생물 유래 β-glucan 분리 · 정제 및 이를 이용한 오염물질 정화용 복합 미생물제재 개발

서현효,이현돈,김형갑 진주산업대학교 산업과학기술연구소 2007 산업과학기술연구소보 Vol.- No.14

β-glucan occuring mainly in mushrooms and fungi are attractive candidates for numerous industrial application due to their pharmacological activity such as antitumor activity via immunomodulating behavior and excellent rheological properties such as emulsification, viscosity, jellation, etc. Screening of β-glucan producing microorganism was performed by congo red dye binding assay of ethanol-precipitates from each culture filtrate. The β-glucan produced form the selected strains was purified mainly by directly diluting the culture broth with the same volume of water, removal of cells by centrifugation at 4,000×g for 20 mim, and precipitation with 3 volumes of ethanol. The monosaccharide composition was analyzed by either TLC or HPLC afer trifuoroacetic acid hydrolysis. The molecular mass of the β-glucan of HH-71 strain was estimated to be about 4,500,000 dalton on a GFC-HPLC chromatogram. A Bacillus protease coated with β-glucan showed 3-times more increased thermal stability comparing to the uncoated control when stored at 30℃ for 1 week. In a similar manner coating of wastewater (containing chlorinated organic compounds) treatment bacteria with β-glucan resulted in significant increase of thermal stability. Although more detailed experimental data need to be accumulated, these preliminary data clearly suggest that β-glucan is applicable as a coating material to stabilize activity or viability of industrial enzymes and microorganisms for treatment of wastewater. Based on these results, β-glucans obtained from HH-71 and HH-87 strains are currently investigated for coating ablility of microbial consortia for the treatment of wastewater.