http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hwanhee Jang,Inkyu Yoo,Jisoo Han,Soogil Chae,Soohyung Lee,Hakhyun Ka 한국발생생물학회 2017 한국발생생물학회 학술발표대회 Vol.2017 No.8
S100 protein family is small calcium-binding proteins with two EF-hand motifs and comprises more than 20 proteins in human. Although S100A proteins are known to play important roles in proinflammatory responses including damage-associated molecular pattern (DAMP) signaling and in the establishment of pregnancy, the expression of S100As have not been determined in the uterine endometrium during the estrous cycle in pigs. Thus, this study was performed to investigate expression and localization of S100A8, S100A9, and S100A12 in the uterine endometrial tissues during the estrous cycle in pigs. Real-time RT-PCR analysis showed that S100A8, S100A9, and S100A12 mRNAs were expressed in the uterine endometrium during the estrous cycle with higher levels on days 15 and 18 of the estrous cycle than the other days of cycle. Immunohistochemistry analysis showed that S100A9 and S100A12 proteins were mainly localized to the immune cells in the uterine endometrium. Especially, S100A9- and S100A12-positive immune cells were detected in the uterine blood vessels on day 15 of the estrous cycle, and also localized to stroma near to luminal epithelium on days 0 and 18 of the estrous cycle. These results showed that S100As were expressed in the uterine endometrium during the estrous cycle in a cyclic stage-specific manner, and these proteins were localized to the immune cells in the endometrium. These suggest that immune cells expressing S100A proteins may be recruited into the endometrium during the estrous cycle and play an important role in regulating endometrial function in pigs.
S100A8, S100A9, and S100A12: Expression and Regulation During the Estrous Cycle in Pigs
Hwanhee Jang,Inkyu Yu,Jisoo Han,Minjeong Kim,Soogil Chae,Suhyung Lee,Hakhyun Ka 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
S100As are calcium-binding proteins with two EF-hand calcium-binding motifs. In several studies, S100A proteins are described to play important roles in pro-inflammatory responses including damage-associated molecular pattern (DAMP) signaling and in the establishment of pregnancy. However, the role of S100As have not been determined in the uterine endometrium during the estrous cycle in pigs. Thus, this study was performed to investigate expression and regulation of S100A8, S100A9, and S100A12 in the uterine endometrial tissues during the estrous cycle in pigs. Real-time RT-PCR analysis showed that S100A8, S100A9, and S100A12 mRNAs were expressed in the uterine endometrium during the estrous cycle with higher levels on days 15 and 18 of the estrous cycle than other days of cycle. To investigate the effects of steroid hormones, estradiol (E2) and progesterone (P4), on expression of S100A8, S100A9, and S100A12 mRNAs, endometrial tissue explants from immature pigs were treated with steroid hormones. Levels of S100A8, S100A9, and S100A12 were increased by the treatment of P4, and the increased levels of S100A8, S100A9, and S100A12 by P4 were not inhibited by the treatment of progesterone receptor antagonist, RU486. However, levels of S100A8, S100A9, and S100A12 were decreased by treatment of MEK inhibitor, U0126. These results exhibited that S100As were expressed in the uterine endometrium during the estrous cycle in a cyclic stage-specific manner, and their expression was affected by P4. These suggest that S100As may play an important role in endometrial function during the proestrous period of the estrous cycle in pigs. [Supported by the Next Generation Biogreen 21 program (#PJ01119103), Rural Development Administration, and by Korea Research Foundation (#2015R1D1A1A01058356)]
Expression and Regulation of S100A15A in the Uterine Endometrium and Placenta in Pigs
Hwanhee Jang,Deokhan Ko,Inkyu Yoo,Jisoo Han,Soogil Chae,Soohyung Lee,Hakhyun Ka 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
S100 protein family is small calcium-binding proteins with EF-hand motif and comprises more than 20 proteins in human. S100 proteins are involved in intracellular and/ or extracellular signaling, such as proliferation, differentiation, apoptosis, calcium homeostasis, inflammation and migration. Previously, we have shown that the expression of S100A15A mRNA in the endometrium is increased on Day 12 of pregnancy compare to the estrous cycle. However, the expression and regulation of S100A15A have not been determined in the uterine endometrium during the estrous cycle and pregnancy in pigs. Therefore, we analyzed the expression and regulation of S100A15A mRNA in the uterine endometrium during the estrous cycle and pregnancy. Real-time RT-PCR analysis showed that S100A15A mRNA was expressed in the uterine endometrium during the estrous cycle and pregnancy with higher levels on Day 12 of pregnancy than the estrous cycle. To investigate the effects of steroid hormones and interleukin 1 beta (IL1B) on the expression of S100A15A mRNA, endometrial tissue explants from Day 12 of the estrous cycle were treated with steroid hormones, estradiol and progesterone, or IL1B. The levels of S100A15A mRNA were increased by estradiol and IL1B. These results showed that S100A15A was expressed in the uterine endometrium in a pregnancy stage-specific manner and the expression of S100A15A was regulated by estradiol and IL1B of conceptus origin, suggesting that S100A15A may play a key role in the establishment of pregnancy in pigs.
Dkk‐1 expression in chondrocytes inhibits experimental osteoarthritic cartilage destruction in mice
Oh, Hwanhee,Chun, Churl‐,Hong,Chun, Jang‐,Soo Wiley Subscription Services, Inc., A Wiley Company 2012 Vol.64 No.8
<P><B>Abstract</B></P><P><B>Objective</B></P><P>Dkk is a family of canonical Wnt antagonists with 4 members (Dkk‐1, Dkk‐2, Dkk‐3, and Dkk‐4). We undertook this study to explore the roles of Dkk‐1 and Dkk‐2 in osteoarthritic (OA) cartilage destruction in mice.</P><P><B>Methods</B></P><P>Expression of Dkk and other catabolic factors was determined at the messenger RNA and protein levels in human and mouse OA cartilage. Experimental OA in mice was induced by destabilization of the medial meniscus (DMM) or by intraarticular injection of <I>Epas1</I> adenovirus (AdEPAS‐1). The role of Dkk in OA pathogenesis was examined by intraarticular injection of AdDkk‐1 or by using chondrocyte‐specific <I>Dkk1</I> (<I>Col2a1‐Dkk1</I>)–transgenic mice and <I>Dkk2</I> (<I>Col2a1‐Dkk2</I>)–transgenic mice. Primary culture mouse chondrocytes were also treated with recombinant Dkk proteins.</P><P><B>Results</B></P><P>We found opposite patterns of <I>Dkk1</I> and <I>Dkk2</I> expression in human and mouse experimental OA cartilage: <I>Dkk1</I> was up‐regulated and <I>Dkk2</I> was down‐regulated. Overexpression of <I>Dkk1</I> by intraarticular injection of AdDkk‐1 significantly inhibited DMM‐induced experimental OA. DMM‐induced OA was also significantly inhibited in <I>Col2a1‐Dkk1</I>–transgenic mice compared with their wild‐type littermates. However, <I>Col2a1‐Dkk2</I>–transgenic mice showed no significant difference in OA pathogenesis. Wnt‐3a, which activates the canonical Wnt pathway, induced <I>Mmp13</I> and <I>Adamts4</I> expression in primary culture chondrocytes, an effect that was significantly inhibited by Dkk‐1 pretreatment or <I>Dkk1</I> overexpression.</P><P><B>Conclusion</B></P><P>Our findings indicate that expression of <I>Dkk1</I>, but not <I>Dkk2</I>, in chondrocytes inhibits OA cartilage destruction. The protective effect of Dkk‐1 appears to be associated with its capacity to inhibit Wnt‐mediated expression of catabolic factors, such as <I>Mmp13</I>, providing evidence that Dkk‐1 might serve as a therapeutic target for OA treatment.</P>
스프링프레임워크를 이용한 신뢰성 평가 커뮤니티 웹 사이트의 설계 및 구현
김환희(Hwanhee Kim),장재준(Jaejun jang),이성복(Songbokg Lee),신경섭(Kyungsub Sin),박은주(Eunju Park),임한규(Hankyu Lim) 한국정보기술학회 2016 Proceedings of KIIT Conference Vol.2016 No.6
현대인들은 스마트폰의 보급과 빠른 인터넷으로 인해 수많은 정보들을 손쉽게 구하고 얻을 수 있다. 하지만 제공되는 정보들이 모두 신뢰성을 가진 것은 아니다. 페이스북이나 인스타그램 등은 ‘좋아요’를 통해 게시글에 대한 관심도의 표현은 가능하지만, 글의 신뢰도를 표현하지는 못한다. 본 논문에서는 게시글의 신뢰도를 표현하기 위해 웹 사이트의 게시글을 평가하는 방법을 제안하였다. 게시글의 평가를 통해 작성자의 신뢰성 평가가 가능하고, 이를 통해 보다 신뢰성 높은 정보의 공유가 가능한 커뮤니티 웹 사이트를 설계하고 구현하였다. In the modern world, people can easily obtain a lot of information due to supply of smartphone and fast internet. But not all information is reliable. By clicking ‘Like’ button in Facebook or Instagram, it is possible to express the interest about the post but it cannot express reliability. This paper suggests the method to evaluate post in web-site which can show its reliability. By evaluating the post, it is possible to evaluate reliability of the writer, and through this, we have designed and developed a community web-site which can share information with high reliability.
차량 주행 시뮬레이터 기반 전방 충돌 회피 지원 시스템 평가 기술 개발
이환희(Hwanhee Lee),장민규(Mingyu Jang),한종철(Jongchul Han),구태윤(Taeyun Koo),문진동(Jindong Moon) 한국자동차공학회 2012 한국자동차공학회 학술대회 및 전시회 Vol.2012 No.11
EDS(Emergency Driving Support) system is important factor for active safety system of intelligent vehicle. For this reason, vehicle manufactures and system are actively developing EDS system. In this paper, a driving simulator with a 6-DOF motion platform is developed and we propose the verification process of EDS system using a driving simulator. And focus on testing method of EDS system. To evaluate performance of EDS system, we propose EDS system TDP(Test Development Procedure) using specification-based test approach and static test respectively.
Oh, Hwanhee,Ryu, Je-Hwang,Jeon, Jimin,Yang, Siyoung,Chun, Churl-Hong,Park, Hongryeol,Kim, Hyung Joon,Kim, Woo-Shin,Kim, Hong-Hee,Kwon, Young-Guen,Chun, Jang-Soo Mary Ann Liebert, Inc 2012 Journal of bone and mineral research Vol.27 No.6
<P>Developing cartilage serves as a template for long-bone development during endochondral ossification. Although the coupling of cartilage and bone development with angiogenesis is an important regulatory step for endochondral ossification, the molecular mechanisms are poorly understood. One possible mechanism involves the action of Dickkopf (DKK), which is a family of soluble canonical Wnt antagonists with four members (DKK1-4). We initially observed opposite expression patterns of Dkk1 and Dkk2 during angiogenesis and chondrocyte differentiation: downregulation of Dkk1 and upregulation of Dkk2. We examined the in vivo role of Dkk1 and Dkk2 in linking cartilage/bone development and angiogenesis by generating transgenic (TG) mice that specifically express Dkk1 or Dkk2 in chondrocytes, hypertrophic chondrocytes, or endothelial cells. Despite specific expression pattern during cartilage development, chondrocyte- and hypertrophic chondrocyte-specific Dkk1 and Dkk2 TG mice showed normal developmental phenotypes. However, Dkk1 misexpression in endothelial cells resulted in defects of endochondral ossification and reduced skeletal size. The defects are caused by the inhibition of angiogenesis in developing bone and subsequent inhibition of apoptosis of hypertrophic chondrocytes and cartilage resorption.</P>