Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium

Huy, N.D.,Nguyen, C.L.,Park, H.S.,Loc, N.H.,Choi, M.S.,Kim, D.H.,Seo, J.W.,Park, S.M. Society for Bioscience and Bioengineering, Japan ; 2016 Journal of bioscience and bioengineering Vol.121 No.2

<P>The cDNA encoding a putative glycoside hydrolase family 5, which has been predicted to be an endoglucanase (PcEg5A), was cloned from Phanerochaete chrysosporium and expressed in Pichia pastoris. PcEg5A contains a carbohydrate-binding domain and two important amino acids, E209 and E319, playing as proton donor and nucleophile in substrate catalytic domain. SDS-PAGE analysis indicated that the recombinant endoglucanase 5A (rPcEg5A) has a molecular size of 43 kDa which corresponds with the theoretical calculation. Optimum pH and temperature were found to be 4.5-6.0, and 50 degrees C-60 degrees C, respectively. Moreover, rPcEg5A exhibited maximal activity in the pH range of 3.0-8.0, whereas over 50% of activity still remained at 20 degrees C and 80 degrees C. rPcEg5A was stable at 60 degrees C for 12 h incubation, indicating that rPcEg5A is a thermostable enzyme. Manganese ion enhanced the enzyme activity by 77%, indicating that rPcEg5A is a metal dependent enzyme. The addition of rPcEg5A to cellobiase (cellobiohydrolase and beta-glucosidase) resulted in a 53% increasing saccharification of NaOH-pretreated barley straw, whereas the glucose release was 47% higher than that cellobiase treatment alone. Our study suggested that rPcEg5A is an enzyme with great potential for biomass saccharification. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.</P>

Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a β-xylosidase that cleaves xylans in synergistic action with endo-xylanase

Huy, N.D.,Nguyen, C.L.,Seo, J.W.,Kim, D.H.,Park, S.M. Society for Bioscience and Bioengineering, Japan ; 2015 Journal of bioscience and bioengineering Vol.119 No.4

A predicted endoglucanase gene (PcGH5) was cloned from Phanerochaete chysosporium, and expressed in Pichia pastoris. Although PcGH5 showed similarity with the conserved domains of a cellulase superfamily GH5, a β-glucosidase/6-phospho-β-glucosidase/β-galactosidase superfamily, and an endoglucanase, recombinant PcGH5 exhibited a β-xylosidase activity, rather than endoglucanase activity. Therefore, the predicted gene was named as PcXyl5. Further characterization of recombinant PcXyl5 showed not only catalysis of the hydrolysis of xylo-oligomers to xylose, but also displayed transglycosylation activity using alcohol as a receptor. Optimum pH of rPcXyl5 was found to be 5.5, whereas optimum temperature was 50<SUP>o</SUP>C. rPcXyl5 increased reducing sugar release of birchwood xylan, beechwood xylan, and arabinoxylan by 6.4%, 13%, 15.8%, respectively, in synergistic action with endo-xylanase. Interestingly, the late addition of rPcXyl5 into reaction with endo-xylanase resulted in a larger increase of reducing sugar release from pretreated barley straw that addition at the start or by treatment with endo-xylanases alone. The increases observed were 6.3% and 13.8%, respectively, showing a great potential application for hemicellulose saccharification.

Magnetic and magnetocaloric properties of Ni-Ag-Mn-Sn ribbons and their composites

Thanh, Tran Dang,Duc, Nguyen Huu,Dan, Nguyen Huy,Mai, N.T.,Phan, T.L.,Oh, S.K.,Yu, Seong-Cho Elsevier 2017 Journal of Alloys and Compounds Vol.696 No.-

<P><B>Abstract</B></P> <P>In this work, we have investigated the influence of Ag-doping on the magnetic and magnetocaloric effect (MCE) of Ni<SUB>50-x</SUB>Ag<SUB>x</SUB>Mn<SUB>37</SUB>Sn<SUB>13</SUB> ribbons with <I>x</I> = 1, 2, and 4, which were prepared by a melt-spinning method. With increasing Ag concentration, a systematic decrease in the antiferromagnetic interaction and in the magnetic phase transition temperatures was observed. Analyses of the critical behavior based on the Banerjee criterion and scaling hypothesis for <I>M</I>(<I>H</I>, <I>T</I>) data near the ferromagnetic-paramagnetic transformation prove an increase of Ag favors establishing long-range ferromagnetic interactions in the austenitic phase. The temperature and magnetic field dependences of magnetic entropy change, Δ<I>S</I> <SUB>m</SUB>(<I>T</I>, <I>H</I>) were investigated via isothermal magnetization measurements. Interestingly, these samples exhibit a MCE at room-temperature with the Δ<I>S</I> <SUB>m</SUB>(<I>T</I>) curves distributed over a quite wide temperature range. To enhance the relative cooling power (RCP) value and to extend the magnetic phase transition region, we have prepared the composites in the form of the layered material samples based on ribbons obtained above. Under Δ<I>H</I> = 10 kOe, the maximum value of Δ<I>S</I> <SUB>m</SUB> (denoted as |Δ<I>S</I> <SUB>max</SUB>|) at around room-temperature is 1.08 J kg<SUP>−1</SUP> K<SUP>−1</SUP>, corresponding to RCP = 51.8 J kg<SUP>−1</SUP>, which is about 10% higher than that obtained from a separate sample. Additionally, we also pointed out that the dependences of |Δ<I>S</I> <SUB>max</SUB>| on Δ<I>H</I> at around room-temperature for samples obey a power law, |Δ<I>S</I> <SUB>max</SUB>| = <I>a</I> × Δ<I>H</I> <SUP>n</SUP>, and all the Δ<I>S</I> <SUB>m</SUB>(<I>T</I>, <I>H</I>) data obey completely a universal master curve.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An increase of Ag in Ni<SUB>1-x</SUB>Ag<SUB>x</SUB>Mn<SUB>37</SUB>Sn<SUB>13</SUB> ribbons favors establishing long-range FM order in the austenitic phase. </LI> <LI> High magnetic entropy change, wide operative temperature range, and high RCP value around room-temperature. </LI> <LI> The composite of Ni<SUB>1-x</SUB>Ag<SUB>x</SUB>Mn<SUB>37</SUB>Sn<SUB>13</SUB> ribbons exhibits higher RCP value and extends the magnetic phase transition region. </LI> <LI> Field dependence of |Δ<I>S</I> <SUB>max</SUB>| can be expressed by the power law. </LI> <LI> All the Δ<I>S</I> <SUB>m</SUB>(<I>T, H</I>) data around room temperature are followed a universal master curve. </LI> </UL> </P>

NOX-VOC-O3 Sensitivity in Urban Environments of Sri Lanka

Perera G.B.S.,Manthilake M.M.I.D.,Sugathapala A.G.T.,Huy L.N.,Lee S.C. 한국대기환경학회 2019 Asian Journal of Atmospheric Environment (AJAE) Vol.13 No.1

Physical phenomenon of the relation among the ground level O3, NOX and VOC governed by complex nonlinear photochemistry in urban environments is explained in detail using the ambient pollutant concentration data of eleven cities in Sri Lanka. The time-series analysis was conducted using the 24-hour average ambient concentrations of PM10, NO2, CO, O3 and SO2 air pollutants obtained from fixed air pollution monitoring station located in Colombo since 2008. Further analysis was carried out from the mobile air pollution monitoring station for eleven cities. The hourly averaged ambient real time air quality data i.e. VOC, NO2, NO, O3 pollutants and the corresponding meteorological parameters were analyzed and presented in weekly results for the base year 2013, 2014 and 2015. It was identified that there exist two regimes of NOX-VOC-O3 sensitivity among these cities. Colombo, Kurunegala, Jaffna, Matara, Badulla, Pollonnaruwa, and Gampaha are the NOX-sensitive regime. While Rathnapura, Anuradhapura, Kandy and Nuwaraelliya are the VOC-sensitive regime. In the NOX-sensitive regime (with relatively low NOX and high VOC), O3 increases with the increasing NOX and slightly changes in response to the increasing VOC levels. In the NOX-saturated or VOC-sensitive regime, O3 decreases with increasing NOX level and increases with increasing VOC levels. In the immediate vicinity of very large emissions of NO, O3 concentrations are depressed through the process of NOX titration. Mathematical relationships were developed to calculate the steady state ozone concentration (O3ss) that gives the values for both NOX-sensitive regime and the VOC-sensitive regime. Establishment of these relationships are essential for Sri Lanka to develop the appropriate interventions for controlling O3 pollution in each city.