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Exploring the Transcriptome Basis of Nitrogen Stress Signals of Apple Leaves using RNA-seq Workflow
Youngsuk Lee,Giap Do Van,Hunjoong Kweon,Seonae Kim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
Ntrogen (N) is one of the main macnonutrients that regulates plant growth and development. In fruit crops such as apple, the imbalanced N nutrition can affect abnormal growth, lack of immunity or alternate fruit bearing since N represents an indicator of tree vigour but little is known for underlying molecular mechanisms of specific metabolic signals derived from N stress responses in apple. To investigate this further, we conducted transcriptome analysis on apple leaves under different N stress conditions including starvation or over-fertilization. A total of 12 RNA-seq libraries were constructed using Illuminar Nextseq 500 platform. 2212 differentially expressed genes (DEGs) were obtained from four different N fertilization groups of apple leaves under the criteria of |fold change| above 1.5 and false discovery rate (FDR) below 0.05. DEGs were further categorized into five clusters of expression dynamics. Functional enrichment analysis revealed N starvation responsive genes were linked to the flavonoid and lipid metabolism while N excessive DEGs were associated with flower development and auxin transport. Our results provide the useful information about metabolic signals and the genes involved in N stress responses in apple. However, further research needs to be investigated.