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Eiichi Uchio,Hirotoshi Inoue,Kazuaki Kadonosono 대한안과학회 2013 Korean Journal of Ophthalmology Vol.27 No.3
Purpose: Antimicrobial peptides have an important role in self-protection of the ocular surface. Human cationic antimicrobial protein (hCAP)-18 is a linear, α-helical peptide that consists of a conserved pro-sequence called a cathelin-like domain and a C-terminal peptide named LL-37. We investigated the in vitro anti-adenoviral activity of hCAP-18/LL-37 in several adenovirus types, inducing keratoconjunctivitis. Methods: A549 cells were used for viral cell culture, and human adenovirus (HAdV) types 3 (HAdV3,species B), 4 (species E), 8, 19a, and 37 (species D) were used. The cytotoxicity of LL-37 was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay to obtain 50% cytotoxic concentration. After pretreatment of A549 cells with serial dilutions of LL-37 for 24hours, adenovirus was cultured for seven days, and adenoviral DNA was quantitatively measured by real-time polymerase chain reaction (PCR). Results: The 50% effective concentration of LL-37 obtained by real-time PCR ranged between 118 and 270 μM. LL-37 showed a significant inhibitory effect on adenoviral proliferation in all adenovirus types except HAdV4 in a dose-dependent manner. Conclusions: LL-37 has significant inhibitory activity against HAdV3, 8, and 19, which induce keratoconjunctivitis. These results indicate that hCAP-18/LL-37 may be a possible candidate for the treatment of HAdV keratoconjunctivitis.
RELATION BETWEEN BONE SCINTIGRAM PATTERNS AND LABORATORY DATA IN PATTENS ON MAINTENANCE HEMODIALYSIS
Ochi, Hironobu,Inoue, Yuichi,Onoyama, Yasuto,Sakamoto, Mariko,Okamura, Terue,Taniguchi, Shuji,Shibakiri, Ippei,Morii, Hirotoshi,Yasumoto, Ryouji,Yamagami, Seiji,Maekawa, Masanobu 대한핵의학회 1984 핵의학 분자영상 Vol.18 No.1