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      • KCI등재

        Knock out of the annexin gene OsAnn3 via CRISPR/Cas9-mediated genome editing decreased cold tolerance in rice

        Chunxiu Shen,Zhiqun Que,Yumei Xia,Ning Tang,Ding Li,Ronghua He,Mengliang Cao 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6

        Plant annexins are Ca2+-dependent phospholipidbindingproteins and exist as multigene families in plants. They are implicated in the regulation of plant developmentas well as protection from environmental stresses. In thisstudy, the rice annexin gene OsAnn3 knockout was performedvia the CRISPR/Cas9 (clustered regularly interspaced shortpalindromic repeats/CRISPR associated proteins) mediatedgenome editing. Thus, mutant plantlets were successfullyobtained. We identified cold tolerance phenotype of T1mutant lines from T0 biallelic mutants using the 4~6°C for 3days cold treatment. The results showed that REC (the relativeelectrical conductivity) of T1 mutant lines was increased, andthe survival ratio of T1 mutant lines was decreased dramaticallycompared with the wild type after the exposure to coldtreatment. It was suggested that OsAnn3 was involved in coldtolerance of rice.

      • Convenient and Robust Route to Photoswitchable Hierarchical Liquid Crystal Polymer Stripes via Flow-Enabled Self-Assembly

        Li, Xiao,Li, Bo,He, Ming,Wang, Wei,Wang, Tianjie,Wang, Aurelia,Yu, Jiwoo,Wang, Zhonglin,Hong, Suck Won,Byun, Myunghwan,Lin, Shaoliang,Yu, Haifeng,Lin, Zhiqun American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.5

        <P>Hierarchically arranged stripes of photoswitchable liquid crystal polymers (LCPs) containing azobenzene moieties were conveniently crafted via a flow-enabled self-assembly (FESA). Interestingly, by subjecting a drop of LCP solution to dry in a restricted geometry comprising two nearly parallel plates with a stationary upper plate and a movable lower plate that programmably traveled in a 'stop -and-move' manner during the FESA process, photoswitchable LCP stripes were yielded, displaying two modes of deposition, namely, periodic primary stripes of large dimensions and regularly spaced secondary stripes of small dimensions situated between adjacent, primary stripes (i.e., forming hierarchical LCP stripes). Notably, these hierarchical azobenzene moieties-containing stripes demonstrated sequential photoinduced reversible phase transition (i.e., photoswitching) due to the thickness difference between primary and secondary stripes. A UV light-induced expansion effect. was observed on the LCP stripes. Clearly, such rapid creation of hierarchical stripes by FESA represents a robust means of organizing polymers, nanoparticles, colloids, DNA, etc. into complex yet ordered patterns over a large area in a simple and controllable manner for potential use in surface relief grating, photoactuators, photoswitchable devices, antifake labels, etc.</P>

      • KCI등재

        Investigation on the Stratigraphic Response and Plugging Effect Induced by Press-In Open Caisson in Mucky Soil

        Yongzhi Wang,Mengbo Liu,Shaoming Liao,Qiong Yi,Junzuo He,Linqi Liu,Zhiqun Gong,Kaiying Li 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.5

        Press-in open caisson method, which sinks into soil with the aid of mechanical pressure without excavation, is becoming an important method of construction in extremely soft foundations. However, many engineering problems are prone to occur in the construction of caissons, such as sinking and large deformation. In this study, the stratigraphic response model, such as soil migration during the press-in process of open caisson, is focused on. Through model tests of soil and caissons, the migration patterns of each part of the soil were carried out, and the mechanism underlying the stratigraphic response pattern was investigated. The three primary conclusions are as follows: 1) The bottom edge foot of caisson produces lateral extrusion on surrounding soil in the range of 10 cm (20%H) below, resulting in lateral movement of soil. 2) two kinds of plug, i.e., “wedged” and “unwedged” plugs exist, and the driven forces for the soil migration can be divided into three stages, i.e., caused by soil plug with active arch, by soil plug with passive arch and caused by soil squeezing effect. 3) there is a significant linear correlation between the soil plug changes, the horizontal movement of the deep soil body, and the displacement of the surface uplift. The soil plugging effect and squeezing effect are interacted and enhanced with each other. This study provides a new understanding of the stratigraphic response in the construction of press-in open caissons, andguides safety control of open caissons construction.

      • KCI등재

        Bright all-solution-processed CsPbBr3 perovskite light emitting diodes optimized by quaternary ammonium salt

        Liu Ning,You Fangtian,Ji Chao,Gong Hongkang,Song Qi,Li Jiasen,Liang Chunjun,He Zhiqun 한국물리학회 2021 Current Applied Physics Vol.31 No.-

        Perovskite light-emitting diodes (PeLEDs) prepared by the all-solution-process are gradually coming into view due to their low cost and flexible production process. However, the performance of CsPbBr3 device is limited by the high non-radiative recombination losses due to incomplete surface coverage and grain defects. Here a quaternary ammonium salt, tetrabutylammonium hexafluorophosphate (TBA-PF6) was simultaneously introduced into perovskite emission layers (CsPbBr3) and electron transport layer (TPBi (1,3,5-Tris(1-phenyl-1Hbenzimidazol- 2-yl) benzene) dissolved in ethyl acetate). As a result, the morphology and luminescence of CsPbBr3 films were improved, and the energy level of TPBi was more conducive to charge transport. Consequently, the maximum luminance and current efficiency of the modified green-emitting PeLEDs are improved. Furthermore, the optimized device had an operating life of more than 20 min at an initial luminance of 1230 cd/ m2. This work provides a simple and easy method to be scaled up for the development of low-cost all-solutionprocessed PeLEDs.

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