Estimation of Acute Infarct Volume with Reference Maps: A Simple Visual Tool for Decision Making in Thrombectomy Cases

Kim, Dong-Eog,Ryu, Wi-Sun,Schellingerhout, Dawid,Jeong, Han&#x2010,Gil,Kim, Paul,Jeong, Sang-Wuk,Park, Man-Seok,Choi, Kang-Ho,Kim, Joon-Tae,Kim, Beom Joon,Han, Moon-Ku,Lee, Jun,Cha, Jae-Kwan,Kim, Dae- Korean Stroke Society 2019 Journal of stroke Vol.21 No.1

<P><B>Background and Purpose</B></P><P>Thrombectomy within 24 hours can improve outcomes in selected patients with a clinical-infarct mismatch. We devised an easy-to-use visual estimation tool that allows infarct volume estimation in centers with limited resources. </P><P><B>Methods</B></P><P>We identified 1,031 patients with cardioembolic or large-artery atherosclerosis infarction on diffusion-weighted images (DWIs) obtained before recanalization therapy and within 24 hours of onset, and occlusion of the internal carotid or middle cerebral artery. Acute DWIs were mapped onto a standard template and used to create visual reference maps with known lesion volumes, which were then used in a validation study (with 130 cases) against software estimates of infarct volume. </P><P><B>Results</B></P><P>The DWI reference map chart comprises 144 maps corresponding to 12 different infarct volumes (0.5, 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, and 19 mL) in each of 12 template slices (Montreal Neurological Institute z-axis –15 to 51 mm). Infarct volume in a patient is estimated by selecting a slice with a similar infarct size at the corresponding z-axis level on the reference maps and then adding up over all slices. The method yielded good correlations to software volumetrics and was easily learned by both experienced and junior physicians, with approximately 1 to 2 minutes spent per case. The sensitivity, specificity, and accuracy for detecting threshold infarct volumes (<21, <31, and <51 mL) were very high (all about >90%). </P><P><B>Conclusions</B></P><P>We developed easy-to-use reference maps that allow prompt and reliable visual estimation of infarct volumes for triaging patients to thrombectomy in acute stroke.</P>

Experience of surgical treatment in a granular cell tumor in the qscending colon: a case report

Kim In-Kyeong,Ju Young-Tae,Kim Han-Gil,Lee Jin-Kwon,Kim Dong-Chul,Kim Jae-Myung,Cho Jin Kyu,Park Ji-Ho,Kim Ju-Yeon,Jeong Chi-Young,Hong Soon-Chan,Kwag Seung-Jin 대한대장항문학회 2023 Annals of Coloproctolgy Vol.39 No.3

We report a case about successful surgical treatment of a granular cell tumor in the ascending colon. A 36-year-old man underwent screening colonoscopy. An endoscopic examination revealed a 10-mm yellowish and hemispheric mass in the ascending colon, and lower endoscopic ultrasonography revealed a hypoechoic-to-isoechoic mass invaded the submucosal layer. The mass was suspected to be a colonic carcinoid tumor. Based on the preoperative evaluation, endoscopic complete resection was considered difficult. Therefore, the lesion was removed via laparoscopic right hemicolectomy. Histological examination revealed that the tumor consisted of nests of polygonal cells with abundant granular eosinophilic cytoplasm. Immunohistochemical staining revealed diffuse positivity for S100 and CD68. Therefore, the tumor was diagnosed as a granular cell tumor. We suggest that surgical resection should be considered if it is located in the thin-walled ascending colon prone to perforation, difficult to rule out malignant tumor due to submucosal invasion, or to remove endoscopically.

In-hospital mortality of atrial fibrillation-associated acute ischemic stroke in the intensive care unit

Kim Do Yeon,Kang Jihoon,Jeong Han-Gil,Park Chan-Young,Kim Jun Yup,Kim Beom Joon,Han Moon-Ku,Bae Hee-Joon,Kim Baik Kyun 대한신경집중치료학회 2022 대한신경집중치료학회지 Vol.15 No.2

Background: Although atrial fibrillation (AF)-associated acute ischemic stroke (AIS) is on the rise, is devastating, and life-threatening, there is limited data on the clinical course and in-hospital mortality of patients treated in the intensive care unit (ICU). This study aimed to describe the clinical course and factors associated with in-hospital mortality in AF-associated AIS patients admitted to the ICU.Methods: This study was a retrospective analysis of a prospective nationwide multicenter cohort including non-valvular AF-AIS patients receiving ICU care admitted to 14 stroke centers in South Korea from 2017 to 2020. In-hospital outcomes, including in-hospital mortality and neurological deterioration (ND) have been described.Result: Amongst 2,487 AF-associated AIS patients, 259 (10.4%) were treated in the ICU. In-hospital mortality and ND occurred in 8.5% and 17.0% of the patients, respectively. Higher rates of initial National Institute for Health Stroke Scale scores, symptomatic steno-occlusive lesions, and CHA₂DS₂-VASc (Congestive Heart Failure, Hypertension, Age ≥75 [Doubled], Diabetes Mellitus, Prior Stroke or Transient Ischemic Attack [Doubled], Vascular Disease, Age 65–74, Female) scores were found in those with in-hospital mortality. The CHA₂DS₂-VASc score after admission increased the risk of in-hospital mortality (odds ratio [OR], 1.48; 95% confidence interval [CI], 1.00–2.18) were associated with in-hospital mortality. Antithrombotic use within 48 hours was related to decreased in-hospital mortality (OR, 0.26; 95% CI, 0.10–0.67).Conclusion: ICU care in AF-associated AIS is common, and the establishment of optimal treatment strategies in the ICU may be needed.

Nocturnal Desaturation is Associated With Neurological Deterioration Following Ischemic Stroke: A Retrospective Observational Study

Kim, Tae Jung,Ko, Sang-Bae,Jeong, Han-Gil,Kim, Chi Kyung,Kim, Yerim,Nam, Kiwoong,Mo, Heejung,An, Sang Joon,Choi, H. Alex,Yoon, Byung-Woo American Academy of Sleep Medicine 2017 Journal of clinical sleep medicine Vol.13 No.11

<P>Conclusions: NOD in the SU was associated with END, especially during nighttime, after ischemic stroke. This suggests that treatment of sleep-disordered breathing could be a modifiable factor to possibly reduce the risk of neurological worsening among acute stroke patients.</P>

Mechanical Behavior of Free-Standing Fuel Cell Electrodes on Water Surface

Kim, Sanwi,Kim, Jae-Han,Oh, Jong-Gil,Jang, Kyung-Lim,Jeong, Byeong-Heon,Hong, Bo Ki,Kim, Taek-Soo American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.24

<P>Fundamental understanding of the mechanical behavior of polymer electrolyte fuel cell electrodes as free-standing materials is essential to develop mechanically robust fuel cells. However, this has been a significant challenge due to critical difficulties, such as separating the pristine electrode from the substrate without damage and precisely measuring the mechanical properties of the very fragile and thin electrodes. We report the mechanical behavior of free-standing fuel cell electrodes on the water surface through adopting an innovative ice-assisted separation method to separate the electrode from decal transfer film. It is found that doubling the ionomer content in electrodes increases not only the tensile stress at the break and the Young's modulus (E) of the electrodes by approximately 2.1-3.5 and 1.7-2.4 times, respectively, but also the elongation at the break by approximately 1.5-1.7 times, which indicates that stronger, stiffer, and tougher electrodes are attained with increasing ionomer content, which have been of significant interest in materials research fields. The scaling law relationship between Young's modulus and density (rho) has been unveiled as E similar to rho(1.6), and it is compared with other materials. These findings can be used to develop mechanically robust electrodes for fuel cell applications.</P>

Proteome analysis of the anti-inflammatory response of flavonoids isolated from Korean Citrus aurantium L. in lipopolysaccharide-induced L6 rat skeletal muscle cells.

Kim, Jin-A,Park, Hyeon-Soo,Park, Kwang-Il,Hong, Gyeong-Eun,Nagappan, Arulkumar,Zhang, Jue,Han, Dae-Yong,Shin, Sung-Chul,Won, Chung-Gil,Kim, Eun-Hee,Kim, Gon-Sup Institute for Advanced Research in Asian Science a 2013 The American journal of Chinese medicine Vol.41 No.4

<P>Skeletal muscle is an important organ in our body and a dynamic composite of proteins. Citrus aurantium L. has been widely used in oriental medicine in Eastern Asia for a long time. It contains over 100 bioactive compounds and flavonoids that regulate the inflammatory response and tumorigenesis, through various mechanisms. In the present study, we investigated changes in the protein pattern using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization time of flight mass spectroscopy (MALDI-TOF/MS) to assess the anti-inflammatory effect of flavonoids isolated from Korean C. aurantium L. in lipopolysaccharide (LPS)-induced L6 cells. L6 skeletal muscle cells were pretreated with flavonoids for 1 h and stimulated with LPS for 24 h. Proteins from the L6 cells of the control, LPS treated and flavonoid treated groups were extracted and resolved by 2-DE using pH 4-7 IPG strips loaded with 150 μg of protein. Forty-one differentially expressed protein spots were identified (more than two-fold was considered significant, p < 0.05), and 18 were detected by MALDI-TOF/MS. These results suggest that proteomics can be used to identify changes in the expression of marker proteins and the anti-inflammatory effect of flavonoids isolated from Korean C. aurantium L.</P>

ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light

Kim, Woe-Yeon,Fujiwara, Sumire,Suh, Sung-Suk,Kim, Jeongsik,Kim, Yumi,Han, Linqu,David, Karine,Putterill, Joanna,Nam, Hong Gil,Somers, David E. Nature Publishing Group 2007 Nature Vol.449 No.7160

The circadian clock is essential for coordinating the proper phasing of many important cellular processes. Robust cycling of key clock elements is required to maintain strong circadian oscillations of these clock-controlled outputs. Rhythmic expression of the Arabidopsis thaliana F-box protein ZEITLUPE (ZTL) is necessary to sustain a normal circadian period by controlling the proteasome-dependent degradation of a central clock protein, TIMING OF CAB EXPRESSION 1 (TOC1). ZTL messenger RNA is constitutively expressed, but ZTL protein levels oscillate with a threefold change in amplitude through an unknown mechanism. Here we show that GIGANTEA (GI) is essential to establish and sustain oscillations of ZTL by a direct protein–protein interaction. GI, a large plant-specific protein with a previously undefined molecular role, stabilizes ZTL in vivo. Furthermore, the ZTL–GI interaction is strongly and specifically enhanced by blue light, through the amino-terminal flavin-binding LIGHT, OXYGEN OR VOLTAGE (LOV) domain of ZTL. Mutations within this domain greatly diminish ZTL–GI interactions, leading to strongly reduced ZTL levels. Notably, a C82A mutation in the LOV domain, implicated in the flavin-dependent photochemistry, eliminates blue-light-enhanced binding of GI to ZTL. These data establish ZTL as a blue-light photoreceptor, which facilitates its own stability through a blue-light-enhanced GI interaction. Because the regulation of GI transcription is clock-controlled, consequent GI protein cycling confers a post-translational rhythm on ZTL protein. This mechanism of establishing and sustaining robust oscillations of ZTL results in the high-amplitude TOC1 rhythms necessary for proper clock function.

Highly photoluminescent N-isopropylacrylamide (NIPAAM) passivated carbon dots for multicolor bioimaging applications

Kim, Min Cheol,Yu, Kwang Sik,Han, Seung Yun,Kim, Jwa-Jin,Lee, Jae Won,Lee, Nam Seob,Jeong, Yeong Gil,Kim, Do Kyung Elsevier 2018 European polymer journal Vol.98 No.-

<P><B>Abstract</B></P> <P>We report a highly luminescent Carbon Dots passivated by nitrogen rich molecules, i.e. N-isopropylacrylamide (NIPAAM), with a QY as high as ca. 94%, which is the highest yield until now. NIPAMM@C-Dots are synthesized directly from the precursors composed of CA, EDA and NIPAAM by hydrothermal reaction. The optical properties of in situ synthesized NIPAAM@C-Dots showed EX-dependent EM at high concentration and EX-independent EM at low concentration. In most cations and anions except Fe<SUP>3+</SUP> and Bi<SUP>3+</SUP> added to the aqueous solution, NIPAAM@C-Dots exhibit an extremely stable inert behavior. In addition, in order to verify its usefulness as a fluorescent marker, C6 glioblastoma cells and NIPAAM@C-Dots were cultured together and then applied by both confocal bioimaging and flow cytometry analysis. The role of NIPAAM present on or around the C-Dots surface can improve QY values and stability, resulting in reduced cytotoxicity and can be a strong candidate as an alternative to organic dyes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Carbon Dots passivated by N-isopropylacrylamide (NIPAAM) with a QY of 94% are synthesized. </LI> <LI> NIPAAM@C-Dots showed EX-independent EM at low concentration. </LI> <LI> In most cations and anions except Fe<SUP>3+</SUP> and Bi<SUP>3+</SUP> added to the aqueous solution, NIPAAM@C-Dots exhibit an extremely stable inert behavior. </LI> <LI> C6 glioblastoma cells and NIPAAM@C-Dots were cultured together and then applied by both confocal bioimaging and flow cytometry analysis. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Mating behavior of Pine Sawyer, Monochamus saltuarius Gebler (Coleoptera: Cerambycidae)

Kim, Min-Ki,Kim, Ju-Sub,Han, Ju-Hwan,Kim, Young-Jae,Yoon, Chang-Mann,Kim, Gil-Hah Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.3

Mating behavior of the pine sawyer, Monochamus saltuarius Gebler (Cerambycidae: Lamiinae) was observed on pine trees in an outdoor net cage kept in one healthy pine tree and a cluster of pinelogs (cut over one month) to attract the beetles. When sawyer adult pairs released into net cage, they all moved to the pinelogs. And then the male was staying motionless with his antenna outstretched while the female was actively moving in his vicinity. The mating behavioral reactions were followed by the next steps: The first phase of courtship was initiated by the female approach toward the male. Second, male dashed, contacted antenna and mounted female's back. Third, female carried the male on her back and walked around and then male began to lick. Fourth, male inserted his penis into the female's genitalia when female stopped walking. While a long pair-bond, the mating pair repeated copulations. lastly, when mating is over, male and female stayed separately. The mating began on sunset and resting of both male and female began at the temperature below about $20^{\circ}C$. Substantially straightforward mate searching by female indicates the presence of a male sex pheromone.

Phenolic Glycosides from the Twigs of <i>Salix glandulosa</i>

Kim, Chung Sub,Kwon, Oh Wook,Kim, Sun Yeou,Choi, Sang Un,Kim, Jae Yoon,Han, Ji Young,Choi, Soo Im,Choi, Jong Gil,Kim, Ki Hyun,Lee, Kang Ro American Chemical Society and American Society of 2014 Journal of natural products Vol.77 No.8

<P>As a part of an ongoing search for bioactive constituents from Korean medicinal plants, the phytochemical investigations of the twigs of <I>Salix glandulosa</I> afforded 12 new phenolic glycosides (<B>1</B>–<B>12</B>) and a known analogue (<B>13</B>). The structures of <B>1</B>–<B>13</B> were characterized by a combination of NMR methods (<SUP>1</SUP>H and <SUP>13</SUP>C NMR, <SUP>1</SUP>H–<SUP>1</SUP>H COSY, HMQC, and HMBC), chemical hydrolysis, and GC/MS. The absolute configuration of <B>13</B> [(1<I>R</I>,2<I>S</I>)-2-hydroxycyclohexyl-2′-<I>O</I>-<I>trans</I>-<I>p</I>-coumaroyl-β-<SMALL>d</SMALL>-glucopyranoside] was determined for the first time. Compounds <B>1</B>–<B>3</B>, <B>6</B>, and <B>7</B> exhibited inhibitory effects on nitric oxide production in lipopolysaccharide-activated murine microglial cells (IC<SUB>50</SUB> values in the range 6.6–20.5 μM).</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2014/jnprdf.2014.77.issue-8/np500488v/production/images/medium/np-2014-00488v_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np500488v'>ACS Electronic Supporting Info</A></P>