Taxonomic Research of the Gobioid Fishes(Perciformes: Gobioidei) in China

Han Lin Wu,Jun Sheng Zhong,I Shiung Chen 韓國魚類學會 2009 韓國魚類學會誌 Vol.21 No.S

Taxonomic Research of the Gobioid Fishes (Perciformes: Gobioidei) in China

Wu, Han-Lin,Zhong, Jun-Sheng,Chen, I-Shiung The Ichthyological Society of Korea 2009 韓國魚類學會誌 Vol.21 No.1

The taxonomic research based on extensive investigations and specimen collections throughout all varieties of freshwater and marine habitats of Chinese waters, including mainland China, Hong Kong and Taiwan, which involved accounting the vast number of collected specimens, data and literature (both within and outside China) were carried out over the last 40 years. There are totally 361 recorded species of gobioid fishes belonging to 113 genera, 5 subfamilies, and 9 families. This gobioid fauna of China comprises 16.2% of 2211 known living gobioid species of the world. This report represents a summary of previous researches on the suborder Gobioidei. A recently diagnosed subfamily, Polyspondylogobiinae, were assigned from the type genus and type species: Polyspondylogobius sinensis Kimura & Wu, 1994 which collected around the Pearl River Delta with high extremity of vertebral count up to 52-54. The undated comprehensive checklist of gobioid fishes in China will be provided in this paper.

Systematic Review on Epstein-Barr Virus (EBV) DNA in Diagnosis of Nasopharyngeal Carcinoma in Asian Populations

Han, Bao-Lin,Xu, Xiang-Ying,Zhang, Chun-Zhi,Wu, Jian-Juan,Han, Chun-Feng,Wang, Hui,Wang, Xuan,Wang, Guang-Shun,Yang, Shu-Juan,Xie, Yao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Objective: To conduct a meta-analysis to investigate the value of EBV DNA in diagnosis of nasopharyngeal cancer (NPC) in Asian populations, and provide important evidence for screening. Methods: Prospective or respective case-control or cohort studies regarding the detection role of EBV DNA for NPC were included in our study. We conducted a comprehensive literature search in PubMed, EMBASE, and the Chinese Biomedical Database (CBM database between January 1980 and March 2012. Results: A total of 18 studies with 1492 NPC cases and 2641 health controls were included. Almost of the included studies were conducted in China, and only one other conducted in Thailand. The overall results demonstrated that the pooled sensitivity, specificity, positive likelihood (+LR) and negative likelihood (-LR) were 0.73 (0.71-0.75), 0.89 (0.88-0.90), 8.84 (5.65-13.84) and 0.19(0.11-0.32), respectively. The overall EBV DNA detection showed the largest area of 0.932 under the summary receiver operator curve (SROC). The accuracy of detection by plasma for NPC (0.86) was higher than in serum (0.81), with largest areas under the SROC of 0.97 and 0.91, respectively. Conclusion: Our results demonstrated the EBV DNA detection in plasma or serum has high sensitivity and specificity in diagnosis of NPC, especially in Chinese populations with a high risk of cancer.

Fault-tolerant converter and fault-tolerant methods for switched reluctance generators

Han, Guoqiang,Liu, Wanli,Lu, Zhe,Wu, Menglin,Lin, Hang The Korean Institute of Power Electronics 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.10

Aiming at improving the fault-tolerant performance of switched reluctance generators, a new power converter with fault-tolerance capability is proposed in this paper. The structure of the converter is simple and there is only one transistor in each of the working modes, which makes fault diagnosis easier. To obtain more fault information, an improved current measurement method is proposed. The phase current is obtained by solving current equations obtained by current sensors and rotor positions. Open-circuit and short-circuit faults can be determined by comparing the consistency of the current slope and gate signal. Fault locations can be determined by comparing the relationships among the magnitude of the bus current, the phase current, and the corresponding thresholds. Open-circuit transistors can be replaced by fault-tolerant strategy, which returns the power converter to normal and maintains the stable operation of the system. Simulations and experiments show that the open-circuit and short-circuit faults of transistors can be diagnosed by the proposed strategy.

Suppression of GHS-R in AgRP Neurons Mitigates Diet-Induced Obesity by Activating Thermogenesis

Wu, Chia-Shan,Bongmba, Odelia Y. N.,Yue, Jing,Lee, Jong Han,Lin, Ligen,Saito, Kenji,Pradhan, Geetali,Li, De-Pei,Pan, Hui-Lin,Xu, Allison,Guo, Shaodong,Xu, Yong,Sun, Yuxiang MDPI AG 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.4

<P>Ghrelin, an orexigenic hormone released primarily from the gut, signals the hypothalamus to stimulate growth hormone release, enhance appetite and promote weight gain. The ghrelin receptor, aka Growth Hormone Secretagogue Receptor (GHS-R), is highly expressed in the brain, with highest expression in Agouti-Related Peptide (AgRP) neurons of the hypothalamus. We recently reported that neuron-specific deletion of GHS-R completely prevents diet-induced obesity (DIO) in mice by activating non-shivering thermogenesis. To further decipher the specific neuronal circuits mediating the metabolic effects of GHS-R, we generated AgRP neuron-specific GHS-R knockout mice (<I>AgRP-Cre</I>;<I>Ghsr<SUP>f/f</SUP></I>). Our data showed that GHS-R in AgRP neurons is required for ghrelin’s stimulatory effects on growth hormone secretion, acute food intake and adiposity, but not for long-term total food intake. Importantly, deletion of GHS-R in AgRP neurons attenuated diet-induced obesity (DIO) and enhanced cold-resistance in mice fed high fat diet (HFD). The HFD-fed knockout mice showed increased energy expenditure, and exhibited enhanced thermogenic activation in both brown and subcutaneous fat; this implies that GHS-R suppression in AgRP neurons enhances sympathetic outflow. In summary, our results suggest that AgRP neurons are key site for GHS-R mediated thermogenesis, and demonstrate that GHS-R in AgRP neurons plays crucial roles in governing energy utilization and pathogenesis of DIO.</P>

A unique cytoplasmic localization of retinoic acid receptor-gamma and its regulations.

Han, Young-Hoon,Zhou, Hu,Kim, Jin-Hee,Yan, Ting-dong,Lee, Kee-Ho,Wu, Hua,Lin, Feng,Lu, Na,Liu, Jie,Zeng, Jin-zhang,Zhang, Xiao-kun American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.27

<P>Recent evidence suggests that extranuclear action of retinoid receptors is involved in mediating the pleiotropic effects of retinoids. However, whether they reside in the cytoplasm remains elusive. Here, we showed that retinoic acid receptor-gamma (RARgamma) was cytoplasmic in confluent cells, or when cells were released from serum depletion or treated with growth factors. In studying the regulation of RARgamma subcellular localization, we observed that ectopically overexpressed RARgamma was mainly cytoplasmic irrespective of serum concentration and cell density. The cytoplasmic retention of RARgamma was inhibited by ligand retinoic acid (RA). In addition, coexpression of retinoid X receptor-alpha (RXRalpha) resulted in nuclear localization of RARgamma through their heterodimerization. Mutagenesis studies revealed that a C-terminal fragment of RXRalpha potently prevents RA-induced RARgamma nuclear localization and transcriptional function. Furthermore, our results showed that the cytoplasmic retention of RARgamma was due to the presence of its unique N-terminal A/B domain, which was subject to regulation by p38 MAPK-mediated phosphorylation. Deletion or mutation of the N-terminal A/B domain largely impaired its cytoplasmic localization. Together, our data demonstrate that the subcellular localization of RARgamma is regulated by complex interactions among ligand binding, receptor phosphorylation, and receptor dimerizations.</P>

Nonlinear dynamic performance of long-span cable-stayed bridge under traffic and wind

Han, Wanshui,Ma, Lin,Cai, C.S.,Chen, Suren,Wu, Jun Techno-Press 2015 Wind and Structures, An International Journal (WAS Vol.20 No.2

Long-span cable-stayed bridges exhibit some features which are more critical than typical long span bridges such as geometric and aerodynamic nonlinearities, higher probability of the presence of multiple vehicles on the bridge, and more significant influence of wind loads acting on the ultra high pylon and super long cables. A three-dimensional nonlinear fully-coupled analytical model is developed in this study to improve the dynamic performance prediction of long cable-stayed bridges under combined traffic and wind loads. The modified spectral representation method is introduced to simulate the fluctuating wind field of all the components of the whole bridge simultaneously with high accuracy and efficiency. Then, the aerostatic and aerodynamic wind forces acting on the whole bridge including the bridge deck, pylon, cables and even piers are all derived. The cellular automation method is applied to simulate the stochastic traffic flow which can reflect the real traffic properties on the long span bridge such as lane changing, acceleration, or deceleration. The dynamic interaction between vehicles and the bridge depends on both the geometrical and mechanical relationships between the wheels of vehicles and the contact points on the bridge deck. Nonlinear properties such as geometric nonlinearity and aerodynamic nonlinearity are fully considered. The equations of motion of the coupled wind-traffic-bridge system are derived and solved with a nonlinear separate iteration method which can considerably improve the calculation efficiency. A long cable-stayed bridge, Sutong Bridge across the Yangze River in China, is selected as a numerical example to demonstrate the dynamic interaction of the coupled system. The influences of the whole bridge wind field as well as the geometric and aerodynamic nonlinearities on the responses of the wind-traffic-bridge system are discussed.

Modeling and assessment of VWNN for signal processing of structural systems

Lin, Jeng-Wen,Wu, Tzung-Han Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.45 No.1

This study aimed to develop a model to accurately predict the acceleration of structural systems during an earthquake. The acceleration and applied force of a structure were measured at current time step and the velocity and displacement were estimated through linear integration. These data were used as input to predict the structural acceleration at next time step. The computation tool used was the Volterra/Wiener neural network (VWNN) which contained the mathematical model to predict the acceleration. For alleviating problems of relatively large-dimensional and nonlinear systems, the VWNN model was utilized as the signal processing tool, including the Taylor series components in the input nodes of the neural network. The number of the intermediate layer nodes in the neural network model, containing the training and simulation stage, was evaluated and optimized. Discussions on the influences of the gradient descent with adaptive learning rate algorithm and the Levenberg-Marquardt algorithm, both for determining the network weights, on prediction errors were provided. During the simulation stage, different earthquake excitations were tested with the optimized settings acquired from the training stage to find out which of the algorithms would result in the smallest error, to determine a proper simulation model.

Transcriptome profile of one-month-old lambs' granulosa cells after superstimulation

Wu, Yangsheng,Lin, Jiapeng,Li, Xiaolin,Han, Bing,Wang, Liqin,Liu, Mingjun,Huang, Juncheng Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.1

Objective: Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte maturation and competence are acquired during follicular development, in which granulosa cells play an essential role. Methods: In this study, we applied RNA sequencing to analyze and compare gene expression between prepubertal and adult superstimulated follicle granulosa cells in sheep. Results: There were more than 300 genes that significantly differed in expression. Among these differently expressed genes, many extracellular matrix genes (EGF containing Fibulin Like Extracellular Matrix Protein 1, pentraxin 3, adrenomedullin, and osteopontin) were significantly down-regulated in the superstimulated follicles. Ingenuity pathway and gene ontology analyses revealed that processes of axonal guidance, cell proliferation and DNA replication were expressed at higher levels in the prepubertal follicles. Epidermal growth factor, T-Box protein 2 and beta-estradiol upstream regulator were predicted to be active in prepubertal follicles. By comparison, tumor protein P53 and let-7 were most active in adult follicles. Conclusion: These results may contribute to a better understanding of the mechanisms governing the development of granulosa cells in the growing follicle in prepubertal sheep.

Lack of Effects of HER-2/neu on Prognosis in Colorectal Cancer: a Meta-analysis

Han, Jun,Meng, Qing-Yang,Liu, Xiao,Xi, Qiu-Lei,Zhuang, Qiu-Lin,Wu, Guo-Hao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: The prognostic value of human epidermal growth factor receptor-2 (HER-2/neu) for survival of patients with colorectal cancer (CRC) is still ambiguous. We therefore performed a meta-analysis to evaluate its prognostic significance. Materials and Methods: We searched the MEDLINE and EMBASE databases for published literature investigating associations between HER-2/neu status and overall survival of patients with CRC. A meta-analysis was performed using a DerSimonian-Laird model and publication bias was investigated by Begg's and Egger's tests. Subgroup analysis was also conducted according to the study design type, study quality score, cut-off value for HER-2/neu overexpression, publication region, patient number and publication year. Results: A total of 17 eligible studies involving 2,347 patients were identified for this meta-analysis. The combined hazard ratio (HR) was 1.31 (95% confidence interval (CI): 0.96-1.79), suggesting that HER-2/neu overexpression was not significantly associated with overall survival of patients with CRC. However, subgroup analysis revealed that HER-2/neu overexpression had an unfavorable impact on survival when the analysis was restricted to subgroups of study quality score ${\leq}5 $(HR=1.56, 95%CI: 1.17-2.10), Asian patients (HR=1.74, 95%CI: 1.22-2.49), patient number ${\leq}106$ (HR=1.57, 95%CI: 1.01-2.44), publication year before 2003 (HR=1.59, 95%CI: 1.02-2.49), and prospectively designed study (HR=3.62, 95%CI: 1.42-9.24). The effect disappeared in subgroups of study quality scores > 5 (HR=0.69, 95%CI: 0.33-1.44), non Asian patients (HR=1.14, 95%CI: 0.77-1.70), patients' number > 106 (HR=1.07, 95%CI: 0.67-1.72), publication year after 2003 (HR=1.13, 95%CI: 0.76-1.69), and retrospectively designed study (HR=1.22, 95%CI: 0.89-1.67). Conclusions: Our meta-analysis suggests that HER-2/neu overexpression might not be a significantly prognostic indicator for patients with CRC. Further studies are required to confirm these results.