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Chanin KHOMLAEM,Hajer ALOUI,Beom Soo KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
A cell retention culture of Paracoccus sp. LL1 was performed in a membrane fermenter equipped with an internal ceramic filter module to reach high cell density and thus enhance the co-production of polyhydroxyalkanoate (PHA) and astaxanthin as growth associated products. This study showed that PHA accumulation increased with increasing dry cell weight (DCW) giving rise to a maximum of 43.87 ± 0.91 g/L (38.76% of DCW) at 48 h. A significant increase in both intra and extracellular astaxanthin concentrations was also recorded during the fermentation process achieving a maximum production of respectively 8.51 ± 0.20 and 10.2 ± 0.24 ㎎/L at 48 h. Based on gas chromatography, Fourier transform infrared spectroscopy, and ¹H nuclear magnetic resonance spectroscopy, the extracted PHA was identified as a copolymer of 3HB/3HV with a 3-hydroxyvalerate (3HV) content of 3.78 ㏖%. The thermal stability of the produced PHA was comparable to that of standard poly-3-hydroxybutyrate (PHB) and higher than that of standard poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) with 12 ㏖% 3HV.