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Chanin KHOMLAEM,Hajer ALOUI,Beom Soo KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
A cell retention culture of Paracoccus sp. LL1 was performed in a membrane fermenter equipped with an internal ceramic filter module to reach high cell density and thus enhance the co-production of polyhydroxyalkanoate (PHA) and astaxanthin as growth associated products. This study showed that PHA accumulation increased with increasing dry cell weight (DCW) giving rise to a maximum of 43.87 ± 0.91 g/L (38.76% of DCW) at 48 h. A significant increase in both intra and extracellular astaxanthin concentrations was also recorded during the fermentation process achieving a maximum production of respectively 8.51 ± 0.20 and 10.2 ± 0.24 ㎎/L at 48 h. Based on gas chromatography, Fourier transform infrared spectroscopy, and ¹H nuclear magnetic resonance spectroscopy, the extracted PHA was identified as a copolymer of 3HB/3HV with a 3-hydroxyvalerate (3HV) content of 3.78 ㏖%. The thermal stability of the produced PHA was comparable to that of standard poly-3-hydroxybutyrate (PHB) and higher than that of standard poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) with 12 ㏖% 3HV.
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( Jianning Zheng ),( Abhishek Negi ),( Chanin Khomlaem ),( Beom Soo Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.6
Bioethanol has attracted much attention in recent decades as a sustainable and environmentally friendly alternative energy source. In this study, we compared the production of bioethanol by Candida molischiana and Saccharomyces cerevisiae at different initial concentrations of cellobiose and glucose. The results showed that C. molischiana can utilize both glucose and cellobiose, whereas S. cerevisiae can only utilize glucose. The ethanol yields were 43-51% from different initial concentrations of carbon source. In addition, different concentrations of microcrystalline cellulose (Avicel) were directly converted to ethanol by a combination of Trichoderma reesei and two yeasts. Cellulose was first hydrolyzed by a fully enzymatic saccharification process using T. reesei cellulases, and the reducing sugars and glucose produced during the process were further used as carbon source for bioethanol production by C. molischiana or S. cerevisiae. Sequential culture of T. reesei and two yeasts revealed that C. molischiana was more efficient for bioconversion of sugars to ethanol than S. cerevisiae. When 20 g/l Avicel was used as a carbon source, the maximum reducing sugar, glucose, and ethanol yields were 42%, 26%, and 20%, respectively. The maximum concentrations of reducing sugar, glucose, and ethanol were 10.9, 8.57, and 5.95 g/l, respectively, at 120 h by the combination of T. reesei and C. molischiana from 50 g/l Avicel.
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