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        Comparative study of the sublethal effects of indoxacarb on juvenile hormone titer and juvenile hormone esterase mRNA expression of Nilaparvata lugens and Chilo suppressalis in rice

        Jing-Lan Liu,Zhenghui Li,Hong-Mei Zhang,Haitao Du,Xu Ding,Yaodong Zhou,Jin-Cai Wu,AndrewWong 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

        Both the rice stem borer, Chilo suppressalis, and the brown planthopper, Nilaparvata lugens are important rice pests. The study is a comparative analysis on the effects of indoxacarb on the pest's juvenile hormone (JH) titer and juvenile hormone esterase (JHE) mRNA expression. RT-PCR and RACE were used to amplify the full length Csjhe (JHE of C. suppressalis) cDNA fromC. suppressalis. The full length cDNA is 1841 bp in length and contains a 1734 bp open reading encoding 577 amino acid residues. An amino acid sequence alignment shows that Csjhe shares high identity with other three other lepidopterans homologues including Manduca sexta, Bombyx mori, and Choristoneura fumiferana. JH III titer in 4th instar larvae of C. suppressalis was significantly reduced with 0.1, 0.2, 0.4 and 0.8 g/L indoxacarb, which were decreased by 11.0%, 22.3%, 32.0% and 37.7% respectively, the expression levels of Csjhe mRNA were 1.38, 1.92 and 5.25 times the control levels in 4th instar larvae with 0.2, 0.4 and 0.8 g/L indoxacarb. Similarly, all of the tested concentrations of indoxacarb caused a significant decrease in JH III titer of N. lugens adult females after indoxacarb treatments, Nljhe (JHE of N. lugens)mRNAaverage expression levels were 4.8, 5.0 and 4.4 times before mating and 6.5, 7.6 and 5.3 times after mating compared to the control at 1, 3 and 5 days, respectively. The comparative studies of the effects of indoxacarb on JH III titer and JHE mRNA further have a significance to a guided integrated pest management.

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        Research and Optimization of Lateral Compressive Performance of the 3-D Printed Beetle Elytron Plate

        Caiqi Zhao,Tengteng Zheng,Lijie Shang,Haitao Lan,Shuo Yang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8

        In this paper, two types of beetle elytron plates (end-trabecular beetle elytron plate (EBEP) and middle-trabecular beetle elytron plate (MBEP)) and honeycomb plate (HP) were manufactured by 3-D printed and applied to large-span spatial structure. The lateral compressive performance of the 3-D printed beetle elytron plate was investigated by lateral compression bearing capacity test and numerical analysis. The influence of number of cylinders, ratio of the radius of the cylinder to the side length of the hexagonal honeycomb core (ratio of radius-length), thickness of core layer and configuration of beetle elytron plate on the lateral compressive performance of the beetle elytron plates were studied and the optimization method for lateral compressive performance of the beetle elytron plates was proposed. The result shows that the lateral compression bearing capacity of EBEP is greater than that of MBEP, and both are greater than of HP. The lateral compression bearing capacity of the beetle elytron plate with six cylinders is about 25% higher than that of the HP without cylinders. The lateral compression bearing capacity of beetle elytron plate can be improved by increasing the thickness of plates. The results of the study will promote the application of beetle elytron plates in large-span spatial structures.

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