덖은 쑥의 열수 추출물에 함유된 휘발성 성분

정하열 안성산업대학교 1996 論文集 Vol.28 No.-

Volatile flavor compounds from hot water extracts of roasted mugworts were analyzed for investigating the principal components which were responsible for characteristic mugwort notes. Nitrogen purge and trap technique was used as a trapping method. Flavor components trapped in tube were thermally desorbed and then purged with helium gas to GC or GC-MS Among the 32 peaks identified were 8 caused by aldehydes, 4 by ketones, 3 by hydrocarbons, 6 by alcohols, 10 by terpenes and 1 by furan. Analysis of the date obtained suggested that monoterpenes were over 50% of flavor components detected and especially the amounts of camphor, borneol and 1,8-cineole were large among them. However, it seemed that the addition of essential oil such as 1,8-cineole and linalool is necessary to enhance the sensory properties of hot water extracts of mugworts which could be used as a base of soft drink.

솔싹 추출물의 향미성분 및 특성

정하열 안성산업대학교 1997 論文集 Vol.29 No.1

음료나 제과와 같은 가공식품의 원료로 사용하기 위해서 열수로 추출하여 제조한 솔싹추출물의 향미성분을 조사하기 위하여 실제 이들을 음용하여 감지하는 경우와 유사한 조건에서 분석하기 위한 NPT 방법을 사용하여 분석하였다. 본 연구에서 휘발성 성분을 Tenax-TA에 흡착시키고 GC-MS에 의하여 분석하여 본 결과, 동정된 전체 화합물은 대부분 carbonyl 화합물과 방향족 화합물 및 알코올류 그리고 monoterpenes류로서 솔잎의 휘발성 성분으로 보고된 화합물들과 유사하였으나 제조공정상 첨가한 수증기 증류 성분에 의하여 Bronyl acetate가 다량 검출되었다. Bronyl acetate는 솔잎에 미량 존재하기는 하지만 본 연구에서와 같이 많이 존재하지는 않는 것으로 본 실험에서 사용한 소재의 기호적 및 생리적특성에 중요한 영향을 미치는 것으로 파악되었다. 이에 의하여 솔싹 추출물은 솔잎 추출물에 비하여 더욱 푸릇하고 상쾌한 관능적 특성을 지니며 또한 이들은 진해, 거담작용이 있는 것으로 알려져 가공식품에 적절하게 사용될 경우에 바람직한 효과를 나타낼것으로 기대된다. 이외에도 솔싹 추출물은 솔잎 추출물에 비하여 아미노산 이나 미량원소의 함량이 많은 것으로 나타났다. Volatile flavor compounds from hot water extracts of pine bud extracts were analyzed for investigating the principal components which were responsible for characteristic pine bud notes. Nitrogen purge and trap technique was used as a trapping method. Flavor components trapped in tube were thermally desorbed and then purged with helium gas to GC or GC-MS. Among the 28 peaks identified were 5 caused by aldehydes, 5 by ketones, 1 by hydrocarbons, 3 by alcohols, and 11 by terpenes. Analysis of the data obtained suggested that monoterpenes were the major flavors components detected and especially the amounts of bornyl acetate were large among them. It seems that the flavors of pine bud extracts are more greeny and coooler than that of pine needle exracts due to the characteristic notes of bornyl acetate contained in pine bud extracts. Besides, pine bud extracts contain larger amounts of total amino acids and minerals than pine needle extracts.

Xanthine Oxidase에 의해 생성된 Superoxide Anion 소거능을 갖는 허브추출물의 검색

정하열 안성산업대학교 1997 論文集 Vol.29 No.2

몇가지 허브류의 식물들에 대하여 xanthine oxidase에 의하여 생성된 활성산소의 소거능을 검색하였다. 검색된 13종류의 허브증에서 mate, eucalyptus, sage, thyme, peppermint 등이 강력한 SOD 유사활성을 나타내었다. 또한 이들 허브들에 함유된 폴리페놀 화합물과 유기산의 존재를 조사하여 허브류의 활성이 이들 화합물에 기인하는 것인지를 예측하고져 하였다. 그 결과, 강한 SOD 유사활성을 나타내는 모든 허브류에서 폴리페놀 화합물과 유기산의 존재가 확인되었으며 따라서 SOD 유사활성이 이들 화합물에 의한 것임을 예측할수 있었다. 이러한 허브류는 훌륭한 향미를 갖고 있을 뿐 아니라 본 연구에서 조사한 바와 같은 생리활성도 보유하고 있으므로 각종 건강식 종의 제조에 적절한 부재료 혹은 첨가물로서 사용될 수 있다고 하겠다. 또한 이들 혀브류에 대해서 암세포에 대한 세포독성 효과나 면역증강효과등을 검토하게 되면 보다 상세한 생리적 활성을 파악할 수 있을 가능성이 있다고 하겠다. Superoxide dismutase (SOD)- like activities of some herbs were examined through the test of eliminating activity of superoxide anion produced by xanthine oxidase. Among the 13 different kinds of herb extracts, mate, eucalyptus, sage, thyme, and peppermint showed the efficacious SOD-like activities. The presence of polyphenol compounds and organic acids in these herbs were tested to find out the active compound groups which exhibited SOD-like activities of these herbs. All of the herbs which showed the efficacious SOD-like activities seemed to contain polyphenolic compounds as well as organic acids. The polyphenolic compounds and/or organic acids contained in these herbs seemed to be the active substances exhibiting the SOD-like activities of these herbs. Therfore, it is expected that these herbs belonging to the so called "medicinal herbs" might be used as an effective ingredients to the physiologically functional foods. In addition, the cytotoxicity to the cancer cell lines or the enhancing activity of immune system of these herbs should be studied in the near future.

포도종실 추출물이 Methylcellulose 필름 성질에 미치는 영향

유승모,박준서,정하열,심재용,김병호,박장우 한국산업식품공학회 2003 산업 식품공학 Vol.7 No.4

The film with methylcellulose(MC) and grape seed extract(GSE) were prepared by casting their aqueous solution at 80℃ and drying at 23℃ for 48 hrs. Effect of grape seed extract(GSE) on the barrier permeabilities and the thermomechanical property of methylcellulose(MC) film was studied. The barrier permeabilities and the thermomechanical property of MC film containing various contents of GSE were evaluated in terms of water vapor, oxygen permeabilities, and dynamic mechanical analysis(DMA). The rate of water vapor permeability of MC film decreased with GSE contnets in the MC films. Also, the rate of oxygen permeability also decreased with GSE contents indicating higher oxygen barrier property of MC film. The results showed that GSE had a good miscibility with MC and acted as a plasticizer for MC film resuling in lowering the glass transition temperature of the film.

Isolation and Characterization of a Water-Soluble Polysaccharide from the Mycelia of Solid Cultured Agaricus blazei Murill

Ha-Yull Chung,Young-Jun Cho,Taewook Kim 한국식품과학회 2005 Food Science and Biotechnology Vol.14 No.2

Isolation and Characterization of a Water-Soluble Polysaccharide from the Mycelia of Solid Cultured Phellinus linteus

Ha-Yull Chung,Taewook Kim 한국식품과학회 2005 Food Science and Biotechnology Vol.14 No.6

Characteristics of Water-soluble Polysaccharide, Showing Inhibiting Activity on α-Glucosidase, in Cordyceps militaris

Ha-Yull Chung,Mi-Kyong Yoo,Hirokazu Kawagishi 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3

Extract of water-soluble polysaccharide (CFWx), showing inhibiting activity on α-glucosidase, was prepared from the fruiting bodies of Cordyceps militaris by hot-water extraction, and ethanol precipitation. Chemical characteristics of CFWx were as follows: carbohydrate content 30% including 16% of uronic acid; 51% protein content; monosaccharide composition, Man:Glu:Gal (30:43:27); molecular weight 3-5×10⁴. CFWx was further purified by ion-exchange, gel-permeation, and affinity chromatography and CFWx-AH-α fraction was isolated. Fundamental structure of CFWx-AH-α was deduced as α-(1→4)-Dglucan with α-(1→3)- and/or α-(1→6)-D-glycosidic side chains based on methylation analysis.

Amino Acid Profile Associated with Lipid Oxidation in Freeze-dried Beef

Ha-Yull Chung,C. W. Seo 한국식품과학회 1993 Food Science and Biotechnology Vol.2 No.1

유지 대체물질로서 Glucitol Fatty Acid Polyesters의 이화학적 특성

정하열(Ha-Yull Chung),윤희남(Hee-Nam Yoon),공운영(Un-Young Kong),김정한(Jung-Han Kim) 한국식품과학회 1993 한국식품과학회지 Vol.25 No.5

포도종실 에탄올 추출물의 항균 활성

정하열(Ha-Yull Chung),박동규(Dong-Kyu Park) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.1

포도종실 에탄올 추출물의 각 검정 균주에 대한 생육 억제 효과를 페이퍼디스크법 및 액체배양법에 의해 조사한 결과, B. subtilis ATCC 9372 혹은 S. aureus ATCC 6538에 대하여는 농도 의존적인 증식억제 효과를 나타냈으며 P. aeruginosa IFO 3080 및 Sal. enteritidis IFO3313에 대해서는 제한적인 증식억제 효과를 나타내기도 하였으나 E. coli ATCC 25922에 대해서는 뚜렷한 항균 효과를 확인하기 어려웠다. 또한 포도종실 에탄올 추출물의 순차 용매 분획물 중에서 에틸아세테이트 분획과 부탄올 분획물만이 각각 35.7 mg/g과 20.2mg/g의 카테킨을 함유하고 있었는데 에틸아세테이트 분획에 비하여 부탄올 분획물이 각 검정 균주에 대해 뚜렷한 항균활성을 나타내었다. 부탄올 분획물은 에틸아세테이트 분획물에 비하여 카테킨의 절대 함량은 적었지만 C18 카트리지로 소분획하였을 때 고분자체 중심의 폴리페놀 화합물로 구성되어 있음을 알 수 있었다. 따라서 포도종실 에탄올 추출물의 항균 활성은 카테킨과 같은 플라반-3-올 화합물의 절대 함량과 더불어 플라반-3-올 화합물의 중합도가 관계하는 것으로 예측되었다. Antimicrobial activities of grape seed ethanol extract and its serial solvent fractions were investigated against various food poisoning microorganisms. The grape seed ethanol extract showed dose dependant antimicrobial activity against Bacillus subtilis ATCC 9372 or Staphylococcus aureus ATCC 6538, whereas had limited effect on Pseudomonas aeruginosa IFO 3080, Salmonella enteritidis IFO3313 and Escherichia coli ATCC 25922. Ethylacetate and butanol fractions among the serial solvent fractions of grape seed ethanol extract contained the catechin at the levels of 35.7 mg/g and 20.2 mg/g, respectively. Nevertheless, the butanol fraction of grape seed ethanol extract showed intense antimicrobial activity compared with the ethylacetate fraction on all microorganisms tested. It was found that the butanol fraction was mainly composed of oligomeric or polymeric polyphenols such as condensed tannins by the fractionation on C18 cartridge according to the difference in the degree of polymerization. Therefore, it seems that the antimicrobial activity of grape seed ethanol extract is related to the degree of polymerization of proanthocyanidin as well as the total content of flavan-3-ol composing the proanthocyanidin.