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RISS 인기검색어
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- 저자 : Gui-Bing Hu (검색결과 5 건)
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Zou, Duo-Bing,Wei, Xiao,Hu, Ruo-Lei,Yang, Xiao-Ping,Zuo, Li,Zhang, Su-Mei,Zhu, Hua-Qing,Zhou, Qing,Gui, Shu-Yu,Wang, Yuan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14
Background: Melatonin, which is mainly produced by the pineal gland, has a good inhibitory effect on cell growth of multiple cancer types. However, the underlying molecular mechanisms of anti-tumor activity for colon cancer have not been fully elucidated. In this study, we investigated the effects of melatonin on migration in human colon cancer RKO cells and the potential molecular mechanisms. Materials and Methods: The viability of RKO cells was investigated by MTT assay after treatment with melatonin, SB203580 (p38 inhibitor) and phorbol 12-myristate 13-acetate (PMA, MAPK activator) alone or in combination for 48h. The effects of melatonin, and ML-7, a selective inhibitor of myosin light chain kinase (MLCK), and SB203580, and PMA on the migration of RKO cells were analyzed by in vitro scratch-wound assay. The relative mRNA levels of MLCK was assessed by real-time quantitative RT-PCR. Western blotting analysis was performed to examine the expression of MLCK, phosphorylation of myosin light chain (pMLC) and p38 (pp38). Results: The proliferation and migration of human colon cancer RKO cells were inhibited significantly after treatment with melatonin. The expression levels of MLCK and phosphorylation of MLC of RKO cells were reduced, and real-time quantitative RT-PCR showed that melatonin had significant effects on suppressing the expression of MLCK. Furthermore, the phosphorylation level of p38, which showed the same trend, was also reduced when cells were treated by melatonin. In addition, ML-7 (25umol/l) could down-regulate the phosphorylation of p38. Conclusions: Melatonin could inhibit the proliferation and migration of RKO cells, and further experiments confirmed that p38 MAPK plays an important role in regulating melatonin-induced migration inhibition through down-regulating the expression and activity of MLCK.
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Liu, Guo-Bing,Qu, Yan-Juan,Liao, Mei-Yan,Hu, Hui-Juan,Yang, Gui-Fang,Zhou, Su-Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Objective: To explore the relationship between computed tomography (CT) manifestations of thymoma and its WHO pathological classification. Methods: One hundred and five histopathologically confirmed cases were collected for their pathological and CT characteristics and results were statistically compared between different pathological types of thymoma. Results: Tumor size, shape, necrosis or cystic change, capsule integrity, invasion to the adjacent tissue, lymphadenopathy, and the presence of pleural effusion were significantly different between different pathological types of thymomas (P<0.05). Type B2, B3 tumors and thymic carcinomas were greater in size than other types. More than 50% of type B3 tumors and thymic carcinomas had a tumor size greater than 10 cm. The shape of types A, AB, and B1 tumors were mostly round or oval, whereas 75% of type B3 tumors and 85% of thymic carcinomas were irregular in shape. Necrosis or cystic change occurred in 67% of type B3 thymomas and 57% of thymic carcinomas, respectively. The respective figures for capsule destruction were 83% and 100%. Increases in the degree of malignancy were associated with increases in the incidence of surrounding tissue invasion: 33%, 75%, and 81% in type B2, type B3, and thymic carcinomas, respectively. Pleural effusion occurred in 48% of thymic carcinomas, while calcification was observed mostly in type B thymomas. Conclusions: Different pathological types of thymic epithelial tumors have different CT manifestations. Distinctive CT features of thymomas may reflect their pathological types.
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( Ji Lin Qing ),( Hai Bing Xiao ),( Lin Zhao ),( Gui Fang Qin ),( Li Hua Hu ),( Zhi Zhong Chen ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4
TIM-1 (also known as KIM-1 and HAVcr-1) is a type I transmembrane glycoprotein member of the TIM family that may play important roles in innate and adaptive immune responses. The overexpression of proteins associated with membrane proteins is a major obstacle to overcome in studies of membrane protein structures and functions. In this study, we successfully coupled the overexpression of the TIM-1 protein with a C-terminal enhanced green fluorescent protein (GFP) tag in Escherichia coli. To the best of our knowledge, this report is the first to describe the overexpression of human TIM-1 in E. coli. The purified TIM-1-EGFP fusion protein recognized and bound directly to apoptotic cells and did not to bind to viable cells. Furthermore, we confirmed that the interactions of TIM-1-EGFP with apoptotic cells were blocked by TIM-1-Fc fusion proteins. This fusion protein represents a readily obtainable source of biologically active TIM-1 that may prove useful in future studies of human TIM-1.
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The mechanism of enhanced photocatalytic activity of SnO2 through fullerene modification
Shuang-Shuang Ding,Wei-Qing Huang,Bing-Xin Zhou,Ping Peng,Wangyu Hu,Meng-Qiu Long,Gui-Fang Huang 한국물리학회 2017 Current Applied Physics Vol.17 No.11
Carbon nanomaterials are prominent building blocks in the synthetic van der Waals (vdW) heterostructures with desired properties. Scientific understanding of their interfacial interactions is the premise to design this kind of vdW heterostructures with optimal performance.We here study the mechanism of enhanced photocatalytic activity of SnO2 by fullerene modification at electronic level, to explore the interfacial interaction and its correlation with photocatalytic activity. The results show that the interfacial interaction increases with the number of C atom of fullerene, and leads to some of C atoms be positively/ negatively charged, making the fullerene a highly active co-catalyst in heterostructures. Compared to pristine SnO2, the band gap of the heterostructures is much smaller, leading to their absorption wavelength extending the entire visible region. Interestingly, a staggered type-II band alignment in the C20 (C60)/SnO2 (101) heterostructures results into the robust separation of photoexcited charge carriers between the two constituents, indicating that the fullerene is an effective sensitizer, and thus enhanced photocatalytic activity. These findings can rationalize the available experiment and will be of broad interest in developing the highly efficient semiconductor photocatalysts via fullerene modification.
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