True Digestibility of Phosphorus in Different Resources of Feed Ingredients in Growing Pigs

Wu, X.,Ruan, Z.,Zhang, Y.G.,Hou, Y.Q.,Yin, Y.L.,Li, T.J.,Huang, R.L.,Chu, W.Y.,Kong, X.F.,Gao, B.,Chen, L.X. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.1

To determine the true digestible phosphorus (TDP) requirement of growing pigs, two experiments were designed with the experimental diets containing five true digestible P levels (0.16%, 0.20%, 0.23%, 0.26% and 0.39%) and the ratio of total calcium to true digestible P (TDP) kept at 2:1. In Experiment 1, five barrows (Duroc${\times}$Landrace${\times}$Yorkshire) with an average initial body weight of 27.9 kg were used in a $5{\times}5$ Latin-square design to evaluate the effect of different dietary P levels on the digestibility and output of P and nitrogen. In Experiment 2, sixty healthy growing pigs (Duroc${\times}$Landrace${\times}$Yorkshire) with an average body weight (BW) of 21.4 kg were assigned randomly to one of the five dietary treatments (12 pigs/diet), and were used to determine the true digestible phosphorus (TDP) requirement of growing pigs on the basis of growth performance and serum biochemical indices. The results indicated that the true digestibility of P increased (p<0.05) linearly with increasing dietary TDP level below 0.26%. The true P digestibility was highest (56.6%) when dietary TDP was 0.34%. Expressed as g/kg dry matter intake (DMI), fecal P output increased (p<0.05) linearly with increasing P input. On the basis of g/kg fecal dry matter (DM), fecal P output was lowest for Diet 4 and highest (p<0.05) for Diet 5. The apparent digestibility of crude protein (CP) did not differ (p>0.05) among the five diets, with the average nitrogen output of 12.14 g/d and nitrogen retention of 66% to 74% (p>0.05), which suggested that there was no interaction between dietary P and CP protein levels. During the 28-d experimental period of Experiment 2, the average daily gain (ADG) of pigs was affected by dietary TDP levels as described by Eq. (1): $y=-809,532x^4+788,079x^3-276,250x^2+42,114x-1,759$; ($R^2=0.99$; p<0.01; y = ADG, g/d; x = dietary TDP, %), F/G for pigs by Eq. (2): $y=3,651.1x^4-3,480.4x^3+1,183.8x^2-172.5x+10.9$ ($R^2=0.99$; p<0.01; y = F/G; x = dietary TDP, %), and Total P concentrations in serum by Eq. (3): $y=-3,311.7x^4+3,342.7x^3-1,224.6x^2+195.6x-8.7$ (R2 = 0.99; p<0.01; y = total serum P concentration and x = dietary TDP, %). The highest ADG (782 g/d), the lowest F/G (1.07) and the highest total serum P concentration (3.1 mmol/L) were obtained when dietary TDP level was 0.34%. Collectively, these results indicate that the optimal TDP requirement of growing pigs is 0.34% of the diet at a total Ca to TDP ratio of 2:1.

Redox-Dependent Spatially Resolved Electrochemistry at Graphene and Graphite Step Edges

Gu&#x308,ell, Aleix G.,Cuharuc, Anatolii S.,Kim, Yang-Rae,Zhang, Guohui,Tan, Sze-yin,Ebejer, Neil,Unwin, Patrick R. American Chemical Society 2015 ACS NANO Vol.9 No.4

<P>The electrochemical (EC) behavior of mechanically exfoliated graphene and highly oriented pyrolytic graphite (HOPG) is studied at high spatial resolution in aqueous solutions using Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+/2+</SUP> as a redox probe whose standard potential sits close to the intrinsic Fermi level of graphene and graphite. When scanning electrochemical cell microscopy (SECCM) data are coupled with that from complementary techniques (AFM, micro-Raman) applied to the same sample area, different time-dependent EC activity between the basal planes and step edges is revealed. In contrast, other redox couples (ferrocene derivatives) whose potential is further removed from the intrinsic Fermi level of graphene and graphite show uniform and high activity (close to diffusion-control). Macroscopic voltammetric measurements in different environments reveal that the time-dependent behavior after HOPG cleavage, peculiar to Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+/2+</SUP>, is not associated particularly with any surface contaminants but is reasonably attributed to the spontaneous delamination of the HOPG with time to create partially coupled graphene layers, further supported by conductive AFM measurements. This process has a major impact on the density of states of graphene and graphite edges, particularly at the intrinsic Fermi level to which Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+/2+</SUP> is most sensitive. Through the use of an improved voltammetric mode of SECCM, we produce movies of potential-resolved and spatially resolved HOPG activity, revealing how enhanced activity at step edges is a subtle effect for Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+/2+</SUP>. These latter studies allow us to propose a microscopic model to interpret the EC response of graphene (basal plane and edges) and aged HOPG considering the nontrivial electronic band structure.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2015/ancac3.2015.9.issue-4/acsnano.5b00550/production/images/medium/nn-2015-00550c_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn5b00550'>ACS Electronic Supporting Info</A></P>

Multisetting Greenberger-Horne-Zeilinger theorem

Ryu, Junghee,Lee, Changhyoup,Yin, Zhi,Rahaman, Ramij,Angelakis, Dimitris G.,Lee, Jinhyoung,&#x17b,ukowski, Marek American Physical Society 2014 Physical review. A. Atomic, molecular, and optical Vol.89 No.2

We present a generalized Greenberger-Horne-Zeilinger (GHZ) theorem, which involves more than two local measurement settings for some parties, and cannot be reduced to one with less settings. Our results hold for an odd number of parties. We use a set of observables, which are incompatible but share a common eigenstate, here a GHZ state. Such observables are called concurrent. The idea is illustrated with an example of a three-qutrit system and then generalized to systems of higher dimensions, and more parties. The GHZ paradoxes can lead to, e.g., secret sharing protocols.

Effect of the Transformed Lactobacillus with Phytase Gene on Pig Production Performance, Nutrient Digestibility, Gut Microbes and Serum Biochemical Indexes

Yin, Q.Q.,Chang, J.,Zuo, R.Y.,Chen, L.Y.,Chen, Q.X.,Wei, X.Y.,Guan, Q.F.,Sun, J.W.,Zheng, Q.H.,Yang, X.,Ren, G.Z. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2

In order to improve the availability of phytase and probiotics together, a phytase gene from Aspergillus ficuum has been expressed in Lactobacillus. In this study, the transformed Lactobacillus with phytase gene was fed to pigs to determine its effect on pig production, feed conversion and gut microbes. Forty eight, 60-day-old, castrated pigs (Duroc${\times}$Landrace${\times}$Pietrain) were assigned to 6 groups, 8 pigs for each group. Group 1 was the control, group 2 was added with chlortetracycline (500 mg/kg), group 3 was added with the transformed Lactobacillus (500 mg/kg) with 20% (w/w) of calcium monohydrogen phosphate (CMP, $CaHPO_{4}$) removed, group 4 was added with the natural Lactobacillus (500 mg/kg) with 20% (w/w) of CMP removed, group 5 was added with the transformed Lactobacillus (500 mg/kg) with 40% (w/w) of CMP removed, group 6 was added with phytase (500 mg/kg) with 40% (w/w) of CMP removed. The results showed: i) the average daily gain (ADG) was improved in groups 2, 3 and 4 (p<0.05); ii) the diarrhea rates in the groups added with Lactobacillus were lower than in the other groups (p<0.05), in which the transformed Lactobacillus had more effect on reducing digestive disease; iii) the transformed Lactobacillus was most effective in improving the digestibilities of crude protein (CP), calcium (Ca), phosphorus (P), compared with the other groups (p<0.05); iv) Lactobacillus could increase lactic acid bacterium number and ammonia concentrations, and decrease pH values and E. coli number in pig feces (p<0.05); v) the phytase activity in the feces of pigs fed with the transformed Lactobacillus was 133.32 U/g, which was higher than in group 4 (9.58 U/g, p<0.05), and was almost the same as group 6 (135.94 U/g); vi) the transformed Lactobacillus could increase serum concentrations of IgA, triglyceride, and glutamic oxaloacetic transaminase activity (p<0.05), and had no significant effect on other serum indexes (p>0.05).

Thermodynamic Analysis of Intergranular Additives in Sintered Nd-Fe-B Magnet

X. G. Cui,X. H. Wang,C. Y. Cui,G. C. Yin,C. D. Xia,X. N. Cheng,X. J. Xu 한국자기학회 2017 Journal of Magnetics Vol.22 No.2

To get deeper insight into the effect of intergranular additives in sintered Nd-Fe-B magnet and consequently improve the properties better, the interaction between additives (oxide, nitride, and carbide) and Nd-rich phase in the temperature range of 298.15-1400 K was analyzed thermodynamically. It can be found that the oxide additives became less stable than nitrides and carbides. Except for calcium oxide, almost all oxides could react with Nd from Nd-rich phase. To be different from oxide additives, the mechanism of nitrides and carbides was defined with various elements, either reaction with Nd from Nd-rich phase or not. The two different mechanisms would show different effects on the microstructure and hence properties of magnet. The thermodynamic analysis had a better agreement with the experimental information.

Expression Analysis of the Mx Gene and Its Genome Structure in Chickens

Yin, C.G.,Du, L.X.,Li, S.G.,Zhao, G.P.,Zhang, J.,Wei, C.H.,Xu, L.Y.,Liu, T.,Li, H.B. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.7

Among the known interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful pathways. The Mx protein has direct antiviral activity and inhibits a wide range of viruses by blocking an early stage of the viral replication cycle. Cloning, characterization, and expression of Mx in vivo and in vitro have been conducted. The chicken Mx gene spans 21 kb and is made up of 14 exons and 13 introns, of which the promoter region was analyzed. The real-time PCR results showed that Mx expression was increased in chicken embryo fibroblasts (CEF) after 12- and 24-h induction with polyI: C. Induction of Mx expression by poly I: C in vivo revealed tissue-specific patterns among the chicken tissues tested. A trace expression of Mx was detected in healthy chicken liver tissues from adult chickens without inducement; the expression levels in the liver, heart, and gizzard were higher than in the muscle and kidney. This is the first report to demonstrate the expression of a glutathione-S-transferase-tagged-Mx fusion protein of 75 KDa, as well as the biological activity tested by SDS-PAGE and western blotting.

Enhanced ferroelectric, piezoelectric and dielectric properties of (1-x)CaBi2Nb2O9-xBaZr0.2Ti0.8O3 high-temperature piezoelectric composite ceramics

Pan C.B.,Zhao G.C.,Li S.M.,Shu M.F.,Wu J.,Wang J.M.Z.,Yin L.H.,Song W.H.,Zhu X.B,Yang J.,Sun Y.P. 한국물리학회 2022 Current Applied Physics Vol.34 No.-

Among Aurivillius layer-structured materials, CaBi2Nb2O9 is a best potential candidate for ultrahigh-temperature applications because of its highest Curie temperature of about 940 ◦C. In this paper, (1-x)CaBi2Nb2O9- xBaZr0.2Ti0.8O3 composite ceramics were prepared by conventional solid-state sintering method. The dielectric results show that the introduction of BaZr0.2Ti0.8O3 not only increases the permittivity of the material, but also reduces its dielectric loss. The optimum electrical properties were obtained in the x = 0.01 sample with piezoelectric coefficient (d33) of 15.1 pC/N and high ferroelectric remnant polarization (Pr) of 9.9 μC/cm2. Furthermore, the composite samples show good thermal depoling performance, the d33 of the x = 0.01 sample is 13.8 pC/N, which is about 91% of the initial value after depoling at 800 ◦C. Therefore, (1-x)CaBi2Nb2O9- xBaZr0.2Ti0.8O3 is one of the candidates for high temperature piezoelectric materials.

Effect of Follicle Stimulation Hormone and Luteinizing Hormone on Cumulus Cell Expansion and <i>In Vitro</i> Nuclear Maturation of Canine Oocytes

Lee, H-S,Seo, Y-I,Yin, X-J,Cho, S-G,Lee, S-S,Kim, N-H,Cho, S-K,Kong, I-K KOCH NEFF UND OETINGER 2007 REPRODUCTION IN DOMESTIC ANIMALS Vol.42 No.6

<P>Contents</P><P>In general, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in the regulation of cumulus cell expansion and oocyte maturation. We investigated the effects of supplementation of FSH or LH in <I>in vitro</I> maturation (IVM) medium on the incidence of cumulus cell expansion and nuclear maturation in canine oocytes. Cumulus–oocyte complexes (COCs) were cultured in TCM-199 supplemented with 10% foetal bovine serum (FBS), 1 mg/ml cysteine, 0.2 m<SMALL>M</SMALL> pyruvic acid and different concentrations of FSH or LH (control, 0.5, 5 or 50 <I>μ</I>g/ml) at 38.5°C, 5% CO<SUB>2</SUB> in air for 72 h. The cumulus cell expansion was measured by microscopic visualization, and nuclear maturation of denuded oocytes was determined by staining with 10 <I>μ</I>g/ml Hoechst33342 for 30 min. The cumulus cell expansion in the 5 <I>μ</I>g/ml FSH group (397.2 ± 64.3 <I>μ</I>m) was significantly higher than those in the control, 0.5, and 50 <I>μ</I>g/ml FSH groups (168.3 ± 19.1, 286.0 ± 69.7 and 300.0 ± 84.3 <I>μ</I>m, respectively; p < 0.05). However, there was no difference in cumulus cell expansion among the control, 0.5, 5 and 50 <I>μ</I>g/ml LH groups (165.6 ± 20.2, 160 ± 26.5, 172 ± 20.5 and 168 ± 23.1 <I>μ</I>m, respectively; p > 0.05). After 72 h of IVM, the proportion of nuclear development to the MI–MII stage in the 0.5 <I>μ</I>g/ml FSH group (15.1%) was higher than those in the control, 0.5 and 50 <I>μ</I>g/ml FSH groups (0.9%, 6.5% and 8.0%, respectively; p < 0.05). However, there was no significant difference in nuclear maturation to the MI-MII stage among control, 0.5, 5 and 50 <I>μ</I>g/ml LH groups (4.6%, 2.3%, 5.4% and 8.6%, respectively; p > 0.05). This study indicated that a FSH supplement in IVM medium can increase cumulus cell expansion and nuclear maturation, while the nuclear maturation rate remained low. Further studies are required to improve the nuclear development to the MI–MII stages in canine oocytes.</P>

ROBUST CONTROL FOR 4WS VEHICLES CONSIDERING A VARYING TIRE-ROAD FRICTION COEFFICIENT

G.-D. YIN,N. CHEN,J.-X. WANG,J.-S. CHEN 한국자동차공학회 2010 International journal of automotive technology Vol.11 No.1

A μ-synthesis for four-wheel steering (4WS) problems is proposed. Applying this method, model uncertainties can be taken into consideration, and a μ-synthesis robust controller is designed with optimized weighting functions to attenuate the external disturbances. In addition, an optimal controller is designed using the well-known optimal control theory. Two different versions of control laws are considered here. In evaluations of vehicle performance with the robust controller, the proposed controller performs adequately with different maneuvers (i.e., J-turn and Fishhook) and on different road conditions (i.e., icy, wet, and dry). The numerical simulation shows that the designed μ-synthesis robust controller can improve the performance of a closed-loop 4WS vehicle, and this controller has good maneuverability, sufficiently robust stability, and good performance robustness against serious disturbances.

Effect of Galacto-mannan-oligosaccharides or Chitosan Supplementation on Cytoimmunity and Humoral Immunity in Early-weaned Piglets

Yin, Y.-L.,Tang, Z.R.,Sun, Z.H.,Liu, Z.Q.,Li, T.J.,Huang, R.L.,Ruan, Z.,Deng, Z.Y.,Gao, B.,Chen, L.X.,Wu, G.Y.,Kim, S.W. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.5

Immunomodulatory feed additives might offer alternatives to antimicrobial growth promoters in pig production. This experiment was designed to determine the effects of dietary galacto-mannan-oligosaccharide (GMOS) and chitosan oligosaccharide (COS) supplementation on the immune response in early-weaned piglets. Forty 15-day-old piglets (Duroc$\times$Landrace$\times$Yorkshire) with an average live body weight of $5.6{\pm}0.51kg$ were weaned and randomly assigned to 4 treatment groups that were fed maize-soybean meal diets containing either basal, 110 mg/kg of lincomycin, 250 mg/kg of COS or 0.2% GMOS, respectively, over a 2-week period. Another six piglets of the same age were sacrificed on the same day at the beginning of the study for sampling, in order to obtain baseline values. Interleukin (IL)-1${\beta}$gene expression in peripheral blood monocytes, jejunal mucosa and lymph nodes, as well as serum levels of IL-1${\beta}$ IL-2 and IL-6, IgA, IgG, and IgM, were evaluated for 5 pigs from each group at 15 and 28 days of age. The results indicate that weaning stress resulted in decreases in serum antibody and cytokine levels. Dietary supplementation with GMOS or COS enhanced (p<0.05) IL-1${\beta}$gene expression in jejunal mucosa and lymph nodes, as well as serum levels of IL-1${\beta}$ IL-2, IL-6, IgA, IgG and IgM compared to supplementation with lincomycin. These findings suggest that GMOS or COS may enhance the cell-mediated immune response in early-weaned piglets by modulating the production of cytokines and antibodies, which shows that GMOS or COS have different effects than the antibiotic on animal growth and health.