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Choi, Eunji,Cho, Eun-Bum,Jaroniec, Mietek The Royal Society of Chemistry 2017 Journal of Materials Chemistry A Vol.5 No.40
<P>Periodic mesoporous organosilicas were prepared using 1,2-bis(triethoxysilyl)ethane (BTEE) in the presence of a poly(ethylene oxide)-<I>b</I>-poly(d,l-lactic acid-<I>co</I>-glycolic acid)-<I>b</I>-poly(ethylene oxide) triblock copolymer (LGE538) template under strongly and weakly acidic conditions. White solid powders were obtained even under weakly acidic conditions using iron chloride hexahydrate. Small angle X-ray scattering (SAXS) and nitrogen adsorption isotherms showed a highly ordered hexagonal (<I>p</I>6<I>mm</I>) mesostructure and a high specific surface area of around 1000 m<SUP>2</SUP>g<SUP>−1</SUP>. The hydrothermal stability of these materials was also investigated because it is a crucial factor in their usage in the chemical industry. The powders were placed in sealed vials inside a convection oven at 373 K for 4 weeks. The hydrothermally treated samples were analyzed every week over the 4 weeks using SAXS and nitrogen adsorption. Solid-state<SUP>29</SUP>Si CP-MAS NMR analysis was also performed to observe the stability of siloxane bonds over the 4 weeks of hydrothermal treatment. The results indicate that the ethane-silica samples show quite good stability, which makes them attractive as catalysts or supporting materials for up to one month.</P>
Choi, Eunji,Oh, Jungju,Lee, Dahee,Lee, Jaewon,Tan, Xiaonan,Kim, Minkyung,Kim, Gyeungyun,Piao, Chunxian,Lee, Minhyung Elsevier 2018 Journal of controlled release Vol.279 No.-
<P><B>Abstract</B></P> <P>The receptor for advanced glycation end-products (RAGE) is involved in tumor angiogenesis. Inhibition of RAGE might be an effective anti-angiogenic therapy for cancer. In this study, a cationic RAGE-binding peptide (RBP) was produced as an antagonist of RAGE, and a ternary-complex consisting of RBP, polyethylenimine (2 kDa, PEI2k), and a suicide gene (pHSVtk) was developed as a gene delivery system with dual functions: the anti-tumor effect of pHSVtk and anti-angiogenic effect of RBP. As an antagonist of RAGE, RBP decreased the secretion of vascular-endothelial growth factor (VEGF) in activated macrophages and reduced the tube-formation of endothelial cells <I>in vitro</I>. In <I>in vitro</I> transfection assays, the RBP/PEI2k/plasmid DNA (pDNA) ternary-complex had higher transfection efficiency than the PEI2k/pDNA binary-complex. In an intracranial glioblastoma animal model, the RBP/PEI2k/pHSVtk ternary-complex reduced α-smooth muscle actin expression, suggesting that the complex has an anti-angiogenic effect. In addition, the ternary-complex had higher pHSVtk delivery efficiency than the PEI2k/pHSVtk and PEI25k/pHSVtk binary-complexes in an animal model. As a result, the ternary-complex induced apoptosis and reduced tumor volume more effectively than the PEI2k/pHSVtk and PEI25k/pHSVtk binary-complexes. In conclusion, due to its dual anti-tumor and anti-angiogenesis effects, the RBP/PEI2k/pHSVtk ternary-complex might be an efficient gene delivery system for the treatment of glioblastoma.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Fabrication of dihydroxyflavone-conjugated hyaluronic acid nanogels for targeted antitumoral effect
Choi, Yu Ri,Kim, Hyun-Jong,Ahn, Guk Young,Lee, Min Jeong,Park, Ju Ri,Jun, Dae-Ryong,Ryu, Tae-Kyoung,Park, Joo Woong,Shin, Eunji,Choi, Sung-Wook Elsevier 2018 Colloids and surfaces Biointerfaces Vol.171 No.-
<P><B>Abstract</B></P> <P>We prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60% for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hyaluronic acid molecules are conjugated among themselves in nanogel form. </LI> <LI> Dihydroxyflavones are conjugated to hyaluronic acid nanogels. </LI> <LI> A low concentration of a conjugation agent leads to the nanogel size reduction. </LI> <LI> The nanogel specifically reduced the viability of tumor cells to approximately 60%. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>